##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630630.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	169171
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.132481335453477	31.0	31.0	33.0	28.0	34.0
2	31.264726223761755	31.0	31.0	34.0	28.0	34.0
3	31.246525704760273	31.0	31.0	34.0	28.0	34.0
4	35.07549757346117	37.0	35.0	37.0	32.0	37.0
5	34.90671569004144	35.0	35.0	37.0	32.0	37.0
6	35.03716949122486	36.0	35.0	37.0	32.0	37.0
7	34.973683432739655	36.0	35.0	37.0	32.0	37.0
8	35.019335465298425	36.0	35.0	37.0	32.0	37.0
9	36.48659049127806	38.0	35.0	39.0	32.0	39.0
10	36.26795963847231	38.0	35.0	39.0	32.0	39.0
11	36.48666733660024	38.0	35.0	39.0	32.0	39.0
12	36.39356036200058	38.0	35.0	39.0	32.0	39.0
13	36.46374378587347	38.0	35.0	39.0	32.0	39.0
14	37.36321237091464	39.0	36.0	40.0	32.0	41.0
15	37.291911734280696	39.0	36.0	40.0	32.0	41.0
16	37.16979269496545	39.0	36.0	40.0	32.0	41.0
17	37.244267634523645	39.0	36.0	40.0	32.0	41.0
18	37.269348765450346	39.0	36.0	40.0	32.0	41.0
19	37.39856121912148	39.0	36.0	40.0	32.0	41.0
20	37.38267197096429	39.0	36.0	40.0	32.0	41.0
21	37.32460646328271	39.0	36.0	40.0	32.0	41.0
22	37.227036548817466	39.0	36.0	40.0	32.0	41.0
23	37.091162196830425	39.0	36.0	40.0	31.0	41.0
24	36.96757127403633	39.0	36.0	40.0	31.0	41.0
25	36.86104001276814	38.0	36.0	40.0	31.0	41.0
26	36.62848242311034	38.0	35.0	40.0	31.0	41.0
27	36.39549922859119	38.0	35.0	40.0	30.0	41.0
28	36.15421673927565	38.0	35.0	40.0	30.0	41.0
29	36.00440382807928	38.0	35.0	40.0	30.0	41.0
30	35.781581949624936	38.0	35.0	40.0	29.0	41.0
31	35.653935958290724	38.0	34.0	40.0	29.0	41.0
32	35.40565463347737	38.0	34.0	40.0	27.0	41.0
33	35.22031553871526	38.0	34.0	40.0	27.0	41.0
34	35.06800219895845	38.0	34.0	40.0	26.0	41.0
35	34.86519557134497	37.0	33.0	40.0	26.0	41.0
36	34.73312801839559	37.0	33.0	40.0	25.0	41.0
37	34.45043181159892	37.0	33.0	40.0	24.0	41.0
38	34.128585868736366	37.0	33.0	40.0	23.0	41.0
39	33.88795360907011	37.0	33.0	40.0	22.0	41.0
40	33.52706433135703	37.0	33.0	40.0	20.0	41.0
41	33.30495179433827	36.0	32.0	40.0	19.0	41.0
42	32.94480732513256	36.0	31.0	39.0	17.0	41.0
43	32.04001276814584	35.0	30.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	0.0
14	2.0
15	1.0
16	5.0
17	16.0
18	20.0
19	68.0
20	106.0
21	184.0
22	311.0
23	531.0
24	793.0
25	1207.0
26	1722.0
27	2412.0
28	3309.0
29	4195.0
30	5325.0
31	6446.0
32	8088.0
33	9900.0
34	12572.0
35	16333.0
36	23199.0
37	30925.0
38	31107.0
39	10393.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.360191758634755	19.384528081054082	13.754130436067646	20.50114972424352
2	20.552576978323707	22.039238403745323	33.24742420391202	24.16076041401895
3	23.025814117076806	25.931749531539094	29.51865272416667	21.52378362721743
4	13.892452016007473	16.548935692287685	32.90634919696638	36.65226309473846
5	12.396923822640996	40.814323968055994	32.342422755673255	14.446329453629758
6	32.28744879441512	33.02220829811256	17.08448847615726	17.60585443131506
7	26.821381915340098	33.10969374183518	21.1762063237789	18.892718019045816
8	31.60411654479787	28.997286769008873	19.143351992953875	20.255244693239387
9	28.40616890601817	11.731916226776457	21.011284440004495	38.850630427200876
10	20.616417707526704	27.552003593996606	28.70882125186941	23.122757446607277
11	33.53116077814756	23.345608880954774	18.848384179321513	24.274846161576157
12	23.36748024188543	29.405749212335447	25.984359021345266	21.24241152443386
13	31.503035390226458	19.05350207777929	27.98411075184281	21.459351780151444
14	23.64412340176508	21.69934563252567	27.602248612350817	27.05428235335844
15	28.50547670700061	24.54853373214085	26.302380431634266	20.643609129224277
16	24.374153962558594	24.779069698707225	27.61347985174764	23.23329648698654
17	22.664641102789485	27.12166978973938	26.64049984926494	23.57318925820619
18	21.840031683917456	21.650873967760432	31.493577504418607	25.015516843903505
19	22.50976822268592	24.83404365996536	31.300873081083637	21.35531503626508
20	23.980469465806785	21.113547830301886	32.967234336854425	21.938748367036904
21	25.68643561839795	22.954288855654926	30.40769399010469	20.951581535842433
22	26.380407989549038	22.80414491845529	29.03038936933635	21.78505772265932
23	23.534175479248805	23.003351638283156	30.773595947295927	22.688876935172104
24	21.646736142719497	25.206447913649505	30.28296812101365	22.863847822617352
25	22.379131174964975	23.904806379343977	30.983442788657626	22.732619657033414
26	23.001578284694187	24.75897169136554	30.213807331043736	22.025642692896536
27	23.30777733772337	24.205685371606243	30.2415898706043	22.244947420066087
28	21.66446967860922	24.039581252105858	31.90203994774518	22.393909121539743
29	21.560432934722854	24.704588847970395	32.11898020346277	21.61599801384398
30	19.868062492980478	26.253908766869028	32.418676959999054	21.459351780151444
31	22.02741604648551	26.24031305602024	29.785837998238467	21.946432899255782
32	20.567946042761466	25.829486141241702	30.906006348605846	22.696561467390982
33	20.184901667543492	25.30398236104297	31.30855761330252	23.202558358111027
34	21.756092947372778	25.697666857794776	30.54365109859255	22.0025890962399
35	20.492874074161644	26.866306872927392	30.662465789053677	21.97835326385728
36	20.52893226380408	27.43969120002837	29.44771858060779	22.583657955559758
37	21.384279811551625	26.192432509117992	30.217945156084667	22.20534252324571
38	20.56144374626857	25.18280319912987	30.37281803618824	23.88293501841332
39	21.377777515058728	26.297651488730338	30.29597271399944	22.02859828221149
40	20.439673466492483	25.993816907153118	29.93775528902708	23.628754337327322
41	19.93840551867637	26.550058816227367	30.280012531698695	23.23152313339757
42	20.103918520313766	26.927783130678424	30.851032387347715	22.117265961660095
43	19.57368579721111	25.552251863499066	29.908790513740534	24.965271825549294
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	8.0
1	14.5
2	21.0
3	115.0
4	209.0
5	209.0
6	354.0
7	499.0
8	617.0
9	735.0
10	985.0
11	1235.0
12	1235.0
13	2004.0
14	2773.0
15	4408.5
16	6044.0
17	5378.5
18	4713.0
19	4713.0
20	4715.5
21	4718.0
22	3341.5
23	1965.0
24	1805.5
25	1646.0
26	1646.0
27	1737.0
28	1828.0
29	2099.5
30	2371.0
31	2650.5
32	2930.0
33	2930.0
34	3492.5
35	4055.0
36	4602.5
37	5150.0
38	6277.5
39	7405.0
40	7405.0
41	8182.5
42	8960.0
43	9815.0
44	10670.0
45	11320.5
46	11971.0
47	11971.0
48	13091.0
49	14211.0
50	13412.0
51	12613.0
52	12274.0
53	11935.0
54	11935.0
55	10658.5
56	9382.0
57	8655.5
58	7929.0
59	7500.0
60	7071.0
61	7071.0
62	6896.5
63	6722.0
64	6159.5
65	5597.0
66	5389.5
67	5182.0
68	5182.0
69	4285.0
70	3388.0
71	2879.5
72	2371.0
73	1794.5
74	1218.0
75	1218.0
76	941.5
77	665.0
78	495.0
79	325.0
80	274.0
81	223.0
82	223.0
83	200.0
84	177.0
85	142.5
86	108.0
87	93.5
88	79.0
89	79.0
90	67.5
91	56.0
92	33.5
93	11.0
94	6.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	169171.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.76096966974245
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.29471847900285	38.01419865106904
2	8.728794276034687	8.16333768790159
3	3.1211286122418023	4.378410011172128
4	1.5738376355775796	2.9437669576936947
5	1.069451116223801	2.5004285604506684
6	0.6914772583621975	1.9400488263354831
7	0.5713852299446313	1.8702969185025804
8	0.34131418602887265	1.2768145840599159
9	0.30465451419614187	1.282134644826832
>10	1.9897352918868354	17.512457808962527
>50	0.19846787854271483	6.635889129933617
>100	0.10997901549819229	8.597809317199756
>500	0.0	0.0
>1k	0.0050565064596870025	4.884406901892168
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2780	1.6433076591141509	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2375	1.4039049246029165	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2068	1.2224317406647711	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1040	0.6147625775103298	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	498	0.2943766957693695	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	440	0.26009185971590876	No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC	403	0.23822049878525278	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	388	0.22935373084039226	No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA	322	0.19033995188300595	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	321	0.18974883402001524	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	294	0.1737886517192663	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	285	0.16846859095235	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	266	0.15723735155552665	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	262	0.15487288010356384	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	249	0.14718834788468474	No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGT	243	0.14364164070674051	No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC	236	0.1395038156658056	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	227	0.1341837548988893	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	221	0.1306370477209451	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	220	0.13004592985795438	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	220	0.13004592985795438	No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG	218	0.12886369413197296	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	207	0.12236139763907525	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	206	0.12177027977608457	No Hit
TCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT	198	0.11704133687215894	Illumina PCR Primer Index 11 (95% over 22bp)
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	193	0.11408574755720544	No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG	190	0.11231239396823332	No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT	187	0.11053904037926121	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	183	0.10817456892729842	No Hit
TAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGC	176	0.1040367438863635	No Hit
GACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTC	173	0.1022633902973914	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0070934143558884204	0.0
3	0.0	0.0	0.0	0.011231239396823333	0.0
4	0.0	0.0	0.0	0.01655130016373965	0.0
5	0.0	0.0	0.0	0.01714241802673035	0.0
6	0.0	0.0	0.0	0.017733535889721052	0.0
7	0.0	0.0	0.0	0.02009800734168386	0.0
8	0.0	0.0	0.0	0.021280243067665263	0.0
9	0.0	0.0	0.0	0.032511482464488595	0.0
10	0.0	0.0	0.0	0.047880546902246836	0.0
11	0.0	0.0	0.0	0.05142725408019105	0.0
12	0.0	0.0	0.0	0.05615619698411666	0.0
13	0.0	0.0	0.0	0.06324961134000508	0.0
14	0.0	0.0	0.0	0.06443184706598648	0.0
15	0.0	0.0	0.0	0.07448085073682842	0.0
16	0.0	0.0	0.0	0.09339662235253086	0.0
17	0.0	0.0	0.0	0.11881469046113105	0.0
18	0.0	0.0	0.0	0.1306370477209451	0.0
19	5.911178629907017E-4	0.0	0.0	0.14659723002169403	0.0
20	5.911178629907017E-4	0.0	0.0	0.16019294087048017	0.0
21	5.911178629907017E-4	0.0	0.0	0.2039356627317921	0.0
22	5.911178629907017E-4	0.0	0.0	0.2648208026198344	0.0
23	5.911178629907017E-4	0.0	0.0	0.3877733181219003	0.0
24	5.911178629907017E-4	0.0	0.0	0.5544685554852782	0.0
25	5.911178629907017E-4	0.0	0.0	0.5946645701686459	0.0
26	5.911178629907017E-4	0.0	0.0	0.6573230636456603	0.0
27	5.911178629907017E-4	0.0	0.0	0.703430256958935	0.0
28	5.911178629907017E-4	0.0	0.0	0.7524930395871633	0.0
29	5.911178629907017E-4	0.0	0.0	0.8287472439129638	0.0
30	5.911178629907017E-4	0.0	0.0	0.9688421774417602	0.0
31	5.911178629907017E-4	0.0	0.0	1.4086338675068422	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGACGAA	25	0.005483266	29.599998	15
ATCTTAC	25	0.005483266	29.599998	1
GTACTAT	25	0.005483266	29.599998	1
CGAAGAG	25	0.005483266	29.599998	18
CATAGGG	35	8.8380306E-4	26.42857	2
CCATAGG	45	1.3167057E-4	24.666666	1
GCCGTCT	70	1.9028266E-7	23.785713	36
GGTCTTC	40	0.0019244221	23.125002	7
CCGTCTT	75	3.701898E-7	22.2	37
AGGGTCT	50	2.688924E-4	22.199999	5
TGTACTG	60	3.70216E-5	21.583332	5
GCTCGGA	60	3.70216E-5	21.583332	11
GGGCAGT	155	0.0	21.483871	7
GCAGTCG	160	0.0	20.8125	9
CCCCGCC	45	0.0038125352	20.555555	7
GTGTCAT	45	0.0038125352	20.555555	24
TAGGGTC	45	0.0038125352	20.555555	4
CAGAGGA	45	0.0038125352	20.555555	4
CTGTGTC	45	0.0038125352	20.555555	22
TTCTCGT	45	0.0038125352	20.555555	11
>>END_MODULE