FastQCFastQC Report
Fri 10 Feb 2017
ERR1630629.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630629.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1297759
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT85650.6599838644925599No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT70640.5443229443987674No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT64040.49346604415765943No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51760.3988413873454162No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC22960.17692036811149064No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT18360.14147464976162755No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA17790.13708246292262277No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA17730.13662012746588542No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15630.12043838648007836No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG14920.11496741690868643No Hit
GGGTACACTGGCTCCAGTGGGGCTCCCTGGGCACCCAGCAGTG14080.1084947205143636No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT13460.10371725412807772No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG855.182119E-821.76470629
TACACGG555.1435863E-420.1818185
CGGACCA1700.018.59
ACGGACC1653.6379788E-1217.9393948
CGAACTA1201.0414806E-716.95833229
GCGTTAT1201.0414806E-716.9583321
AAGACGG1757.2759576E-1216.9142865
TCTAGCG1107.8095036E-716.81818228
CGCAATA1107.8095036E-716.81818236
GACGGAC1708.54925E-1116.323537
CTAGACT1403.473724E-815.8571434
GGTATCA41500.015.7807231
CGTTATT957.060796E-515.5789482
CGGCGCA2150.015.48837233
GCAATAC1353.9730003E-715.07407337
GGTATAC750.00410478514.83
GTTATAC1001.0935366E-414.7999993
GCTTTAT6850.014.5839411
TATTGAG6950.014.3741015
GCGAACT1551.210301E-714.32258128
Produced by FastQC (version 0.11.5)