##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630629.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1297759
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.10083305143713	33.0	31.0	34.0	30.0	34.0
2	32.25196357721272	33.0	31.0	34.0	30.0	34.0
3	32.348066936927424	34.0	31.0	34.0	30.0	34.0
4	35.91549432521755	37.0	35.0	37.0	35.0	37.0
5	35.879339692500686	37.0	35.0	37.0	35.0	37.0
6	35.960783936000446	37.0	35.0	37.0	35.0	37.0
7	35.90821870624669	37.0	35.0	37.0	35.0	37.0
8	35.9218637666932	37.0	35.0	37.0	35.0	37.0
9	37.62193596808036	39.0	37.0	39.0	35.0	39.0
10	37.54891085324779	39.0	37.0	39.0	35.0	39.0
11	37.641670757051195	39.0	37.0	39.0	35.0	39.0
12	37.595639097860236	39.0	37.0	39.0	35.0	39.0
13	37.63819630609381	39.0	37.0	39.0	35.0	39.0
14	38.932509811143674	40.0	38.0	41.0	36.0	41.0
15	38.93599427936928	40.0	38.0	41.0	36.0	41.0
16	38.88696591585957	40.0	38.0	41.0	35.0	41.0
17	38.90255278522437	40.0	38.0	41.0	35.0	41.0
18	38.901355336391426	40.0	38.0	41.0	35.0	41.0
19	38.960909537132856	40.0	38.0	41.0	35.0	41.0
20	38.93781665162792	40.0	38.0	41.0	35.0	41.0
21	38.89886334827961	40.0	38.0	41.0	35.0	41.0
22	38.8222166057026	40.0	38.0	41.0	35.0	41.0
23	38.77514315061579	40.0	38.0	41.0	35.0	41.0
24	38.70868936374165	40.0	38.0	41.0	35.0	41.0
25	38.66198731813842	40.0	38.0	41.0	34.0	41.0
26	38.50905907799522	40.0	38.0	41.0	34.0	41.0
27	38.36350817062336	40.0	38.0	41.0	34.0	41.0
28	38.254167376223165	40.0	38.0	41.0	34.0	41.0
29	38.18212472423616	40.0	38.0	41.0	34.0	41.0
30	38.08087248865159	40.0	37.0	41.0	33.0	41.0
31	38.0033580965341	40.0	37.0	41.0	33.0	41.0
32	37.89739389208628	40.0	37.0	41.0	33.0	41.0
33	37.787576892165646	40.0	37.0	41.0	33.0	41.0
34	37.70988527145641	40.0	37.0	41.0	33.0	41.0
35	37.61462567395025	40.0	37.0	41.0	33.0	41.0
36	37.55579348708042	40.0	37.0	41.0	33.0	41.0
37	37.38609711048045	40.0	36.0	41.0	32.0	41.0
38	37.20583405701675	40.0	36.0	41.0	32.0	41.0
39	37.1079368357299	40.0	36.0	41.0	31.0	41.0
40	36.88477521635373	40.0	35.0	41.0	31.0	41.0
41	36.784549365483116	40.0	35.0	41.0	31.0	41.0
42	36.6069378058638	39.0	35.0	41.0	30.0	41.0
43	35.83456635631115	38.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	0.0
10	3.0
11	4.0
12	1.0
13	2.0
14	1.0
15	3.0
16	4.0
17	16.0
18	27.0
19	69.0
20	135.0
21	314.0
22	603.0
23	1036.0
24	1811.0
25	2829.0
26	4607.0
27	6704.0
28	9855.0
29	13776.0
30	18560.0
31	24010.0
32	31005.0
33	40092.0
34	52804.0
35	70344.0
36	99582.0
37	158407.0
38	282763.0
39	478390.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.92467399571107	19.360990754061426	11.986971386829142	23.727363863398367
2	18.980180449528763	21.253175666668465	33.85428265186371	25.91236123193906
3	21.78794367829466	23.984961768710523	28.347636194393566	25.879458358601248
4	14.670982825008341	15.222163745348713	33.883795065185446	36.2230583644575
5	14.165033723518775	37.89917850694929	33.60739551796597	14.328392251565969
6	35.11799956694579	33.42800936075188	15.712239329490298	15.741751742812033
7	28.620337057959144	30.767962310413566	21.69532247512828	18.91637815649901
8	28.605542323343546	31.608719338490427	20.143262346861015	19.642475991305012
9	26.983207205652203	13.667098436612655	20.938633444268156	38.41106091346698
10	18.148207795129913	27.340746625529082	32.044778730103204	22.4662668492378
11	34.05871197965107	22.46565040196215	21.40566931148233	22.069968306904443
12	22.653281541488056	26.512010319327395	28.436404602087133	22.398303537097412
13	30.304316903215465	20.244513811886492	25.872831550388014	23.57833773451003
14	22.892231916711808	21.594148066012256	26.50700168521274	29.006618332063194
15	26.069478231320296	27.58347274031619	23.574253771308847	22.77279525705466
16	24.21828706254397	26.42231724072035	25.700919816391178	23.6584758803445
17	23.14304890199182	26.835645139043535	26.373849073672385	23.64745688529226
18	22.461104103304237	24.96341770698566	28.763738105457175	23.811740084252932
19	23.68606189592983	25.647982406594753	28.83609360443657	21.829862093038845
20	24.467100594178117	24.629765619040207	28.14736788571684	22.755765901064837
21	24.371397154633488	25.0135040481322	27.989557383150494	22.625541414083816
22	24.24864709086972	25.294064614462314	28.09003828908141	22.367250005586552
23	23.72528335384305	25.34723319198711	27.57977405666229	23.34770939750755
24	22.90548553313828	26.060616801732834	28.31596621560706	22.71793144952183
25	23.274043948067398	26.041969271644426	27.62061368867409	23.063373091614082
26	23.17425654532159	26.32861725482158	27.80870716365673	22.6884190362001
27	23.399876248209413	25.490557183575685	27.871584785773013	23.237981782441885
28	22.752067217410936	26.127347219321923	27.90656816866614	23.214017394601
29	22.76871129385348	26.55177116860681	28.39163511869307	22.287882418846642
30	22.960118172942742	26.47440703551276	27.642266399231293	22.92320839231321
31	22.072357040097586	27.197268522121597	28.129259746994627	22.601114690786194
32	22.057099970025252	26.469629569126475	27.995953023635362	23.477317437212918
33	21.739475511246695	26.97172587514323	28.134114269290368	23.15468434431971
34	23.338000352916065	25.686048025866125	27.825890631465473	23.150060989752337
35	22.4303587954312	26.027636872485573	27.956731565722144	23.585272766361086
36	21.851668915414958	26.767912994631516	28.14220513978327	23.238212950170258
37	22.54124224913871	25.484084487181363	28.62935259936552	23.345320664314407
38	22.532689043189066	25.766109115791146	28.281830447717947	23.419371393301837
39	22.245193445007892	25.496336376784907	28.753952004956236	23.504518173250965
40	21.482339941391277	25.271641344810554	29.162733604621504	24.083285109176668
41	20.666857251616054	25.69745230046565	29.651422182392878	23.98426826552542
42	21.124029962419833	25.535249611060294	29.877041885280704	23.463678541239165
43	21.32491471837221	25.364647827524216	29.378027815642195	23.932409638461376
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	673.0
1	558.5
2	444.0
3	1055.5
4	1667.0
5	1667.0
6	2291.0
7	2915.0
8	2817.0
9	2719.0
10	4187.5
11	5656.0
12	5656.0
13	9624.5
14	13593.0
15	17115.5
16	20638.0
17	19988.0
18	19338.0
19	19338.0
20	21867.5
21	24397.0
22	21841.0
23	19285.0
24	20514.5
25	21744.0
26	21744.0
27	24405.5
28	27067.0
29	30029.0
30	32991.0
31	36419.0
32	39847.0
33	39847.0
34	43772.5
35	47698.0
36	51247.0
37	54796.0
38	58619.5
39	62443.0
40	62443.0
41	65017.5
42	67592.0
43	68179.0
44	68766.0
45	71037.0
46	73308.0
47	73308.0
48	78463.5
49	83619.0
50	83240.5
51	82862.0
52	81718.0
53	80574.0
54	80574.0
55	77956.5
56	75339.0
57	73777.5
58	72216.0
59	73015.0
60	73814.0
61	73814.0
62	67985.5
63	62157.0
64	57154.5
65	52152.0
66	45087.5
67	38023.0
68	38023.0
69	33772.0
70	29521.0
71	24844.5
72	20168.0
73	14805.5
74	9443.0
75	9443.0
76	6827.0
77	4211.0
78	3350.0
79	2489.0
80	2025.0
81	1561.0
82	1561.0
83	1211.5
84	862.0
85	718.0
86	574.0
87	479.5
88	385.0
89	385.0
90	283.5
91	182.0
92	101.0
93	20.0
94	13.5
95	7.0
96	7.0
97	5.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1297759.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.51831114357271
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.29112075468014	53.432295170464826
2	7.293724501280013	8.82807777527927
3	1.7009111318023837	3.088088073059492
4	0.7054964970382189	1.7078182607383825
5	0.40238169206851754	1.2175730219539904
6	0.2595230314069841	0.9423537338166633
7	0.18879865764502124	0.7998043134795182
8	0.13328051469501226	0.6452729326150615
9	0.10754216504195258	0.5857443184556101
>10	0.7354290645838911	8.761502698675567
>50	0.09042937455961522	3.8675910525039394
>100	0.08229025682450501	9.671840617106273
>500	0.006516764465660484	2.6082674030840556
>1k	0.0020444751264817205	1.7396955666394498
>5k	5.111187816204301E-4	2.1040750621279627
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	8565	0.6599838644925599	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7064	0.5443229443987674	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6404	0.49346604415765943	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5176	0.3988413873454162	No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC	2296	0.17692036811149064	No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT	1836	0.14147464976162755	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	1779	0.13708246292262277	No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA	1773	0.13662012746588542	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1563	0.12043838648007836	No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG	1492	0.11496741690868643	No Hit
GGGTACACTGGCTCCAGTGGGGCTCCCTGGGCACCCAGCAGTG	1408	0.1084947205143636	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1346	0.10371725412807772	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	7.705590945622416E-5	0.0
2	0.0	0.0	0.0	9.246709134746898E-4	0.0
3	0.0	0.0	0.0	0.001541118189124483	0.0
4	0.0	0.0	0.0	0.002928124559336518	0.0
5	0.0	0.0	0.0	0.003467515925530087	0.0
6	0.0	0.0	0.0	0.003467515925530087	0.0
7	0.0	0.0	0.0	0.003621627744442535	0.0
8	0.0	0.0	0.0	0.0037757395633549835	0.0
9	0.0	0.0	0.0	0.006549752303779053	0.0
10	0.0	0.0	0.0	0.009940212319852915	0.0
11	0.0	0.0	0.0	0.011481330508977398	0.0
12	0.0	0.0	0.0	0.013022448698101883	0.0
13	0.0	0.0	0.0	0.014255343249401468	0.0
14	0.0	0.0	0.0	0.01618174098580707	0.0
15	0.0	0.0	0.0	0.019032809635687365	0.0
16	0.0	0.0	0.0	0.024657891025991727	0.0
17	0.0	0.0	0.0	0.0319011465148768	0.0
18	0.0	0.0	0.0	0.03482927107421332	0.0
19	0.0	0.0	0.0	0.04130196746853614	0.0
20	0.0	0.0	0.0	0.045154762941347355	0.0
21	0.0	0.0	0.0	0.05440147207609425	0.0
22	0.0	0.0	0.0	0.06865681532549572	0.0
23	0.0	0.0	0.0	0.09185064407181918	0.0
24	0.0	0.0	0.0	0.13184266107959952	0.0
25	0.0	0.0	0.0	0.1438633829547705	0.0
26	0.0	0.0	0.0	0.1636667516850201	0.0
27	0.0	0.0	0.0	0.19125276727034835	0.0
28	0.0	0.0	0.0	0.2478888607206731	0.0
29	0.0	0.0	0.0	0.332419193394151	0.0
30	0.0	0.0	0.0	0.4418385848219893	0.0
31	0.0	0.0	0.0	0.7734101632121219	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGCGG	85	5.182119E-8	21.764706	29
TACACGG	55	5.1435863E-4	20.181818	5
CGGACCA	170	0.0	18.5	9
ACGGACC	165	3.6379788E-12	17.939394	8
CGAACTA	120	1.0414806E-7	16.958332	29
GCGTTAT	120	1.0414806E-7	16.958332	1
AAGACGG	175	7.2759576E-12	16.914286	5
TCTAGCG	110	7.8095036E-7	16.818182	28
CGCAATA	110	7.8095036E-7	16.818182	36
GACGGAC	170	8.54925E-11	16.32353	7
CTAGACT	140	3.473724E-8	15.857143	4
GGTATCA	4150	0.0	15.780723	1
CGTTATT	95	7.060796E-5	15.578948	2
CGGCGCA	215	0.0	15.488372	33
GCAATAC	135	3.9730003E-7	15.074073	37
GGTATAC	75	0.004104785	14.8	3
GTTATAC	100	1.0935366E-4	14.799999	3
GCTTTAT	685	0.0	14.583941	1
TATTGAG	695	0.0	14.374101	5
GCGAACT	155	1.210301E-7	14.322581	28
>>END_MODULE