FastQCFastQC Report
Fri 10 Feb 2017
ERR1630628.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630628.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1003819
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA14080.14026433052173748No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13510.13458601600487735No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC11420.11376552944305696No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAAT450.00382547620.5555551
ATACCGT1351.8189894E-1220.5555556
CCTATAG656.901015E-519.9230773
GGTATCA11100.019.1666661
TACCGTC1457.2759576E-1219.137937
CTTACAC1205.171387E-918.53
CTGTACG500.00703427818.4999984
TAGCGAG500.00703427818.49999837
CTAGCGG1800.017.47222129
GTATTAA1603.45608E-1117.343751
CGTCGTA1502.5102054E-1017.26666610
GTCCTAT1950.017.0769231
ATTCCGC1201.04078936E-716.95833228
ACCGTCG1554.0017767E-1016.7096778
GTATAGA904.4450542E-516.4444451
CTATGCT803.3820313E-416.18754
TCGATGC1606.2937033E-1016.187529
ATTTTCG803.3820313E-416.187515
CGCAATA1851.8189894E-1116.036
CTAGACT1059.340658E-615.8571444
Produced by FastQC (version 0.11.5)