##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630628.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1003819
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.965261665698698	33.0	31.0	34.0	30.0	34.0
2	32.13566489576308	33.0	31.0	34.0	30.0	34.0
3	32.24895822852526	34.0	31.0	34.0	30.0	34.0
4	35.83192189030094	37.0	35.0	37.0	35.0	37.0
5	35.80294455474543	37.0	35.0	37.0	35.0	37.0
6	35.837833314571654	37.0	35.0	37.0	35.0	37.0
7	35.80719233248225	37.0	35.0	37.0	35.0	37.0
8	35.79773843690944	37.0	35.0	37.0	35.0	37.0
9	37.52948290478662	39.0	37.0	39.0	35.0	39.0
10	37.42669246148957	39.0	37.0	39.0	34.0	39.0
11	37.54107463596525	39.0	37.0	39.0	35.0	39.0
12	37.45711926154018	39.0	37.0	39.0	35.0	39.0
13	37.51496534733852	39.0	37.0	39.0	35.0	39.0
14	38.77541767988053	40.0	38.0	41.0	35.0	41.0
15	38.75415886728584	40.0	38.0	41.0	35.0	41.0
16	38.73152231627415	40.0	38.0	41.0	35.0	41.0
17	38.70402931205725	40.0	38.0	41.0	35.0	41.0
18	38.68398187322615	40.0	38.0	41.0	34.0	41.0
19	38.69148422175711	40.0	38.0	41.0	34.0	41.0
20	38.68034874813089	40.0	38.0	41.0	34.0	41.0
21	38.603626749443876	40.0	38.0	41.0	34.0	41.0
22	38.54507336481975	40.0	38.0	41.0	34.0	41.0
23	38.515412639131156	40.0	38.0	41.0	34.0	41.0
24	38.46674549893955	40.0	38.0	41.0	34.0	41.0
25	38.423487700471895	40.0	38.0	41.0	34.0	41.0
26	38.240430794794676	40.0	38.0	41.0	34.0	41.0
27	38.12013221507065	40.0	38.0	41.0	33.0	41.0
28	38.01544202689927	40.0	38.0	41.0	33.0	41.0
29	37.91564813975428	40.0	37.0	41.0	33.0	41.0
30	37.85798136915121	40.0	37.0	41.0	33.0	41.0
31	37.83051127743149	40.0	37.0	41.0	33.0	41.0
32	37.73414928388485	40.0	37.0	41.0	32.0	41.0
33	37.69749227699416	40.0	37.0	41.0	32.0	41.0
34	37.64066828780886	40.0	37.0	41.0	32.0	41.0
35	37.597774100709394	40.0	37.0	41.0	32.0	41.0
36	37.56175366276191	40.0	37.0	41.0	32.0	41.0
37	37.39197803588097	40.0	36.0	41.0	31.0	41.0
38	37.27582163716766	40.0	36.0	41.0	31.0	41.0
39	37.224479711979946	39.0	36.0	41.0	31.0	41.0
40	37.12316164567517	39.0	36.0	41.0	31.0	41.0
41	37.02531731318096	39.0	35.0	41.0	31.0	41.0
42	36.94095648717548	39.0	35.0	41.0	31.0	41.0
43	36.02744319444043	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	4.0
17	17.0
18	25.0
19	59.0
20	125.0
21	275.0
22	478.0
23	905.0
24	1466.0
25	2414.0
26	3742.0
27	5506.0
28	7747.0
29	11026.0
30	14854.0
31	19433.0
32	25102.0
33	32872.0
34	43289.0
35	58924.0
36	82430.0
37	124371.0
38	228926.0
39	339827.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.51579418201887	17.753698624951312	11.480655377114799	26.249851815915022
2	20.908251387949424	19.46994428278405	31.93284845176272	27.688955877503812
3	21.889802842942803	20.945210242085476	27.84256922811782	29.322417686853907
4	15.470916569620618	14.17835287038799	32.59033750108336	37.76039305890803
5	16.249941473512656	35.02244926625218	33.046196575279005	15.681412684956154
6	38.27751815815401	32.03037599407861	14.297497855689123	15.394607992078251
7	31.444314164206894	28.4838202903113	19.590384322273238	20.48148122320857
8	29.530224074260396	30.333855007725496	18.811757896592912	21.324163021421192
9	27.612348441302665	12.823726189681606	18.55165124389955	41.012274125116186
10	18.532325050631638	24.990361808254278	31.398389550307375	25.078923590806706
11	38.4001498278076	19.825187608523052	19.57852959547488	22.196132968194465
12	23.897136834429315	24.004028614720383	26.445305378758523	25.653529172091783
13	32.42516828232978	17.999758920681916	23.0381174295366	26.536955367451704
14	24.387065795726123	19.83843700906239	23.00813194410546	32.76636525110603
15	28.189842989622633	25.890524088505995	20.353270858591042	25.566362063280334
16	27.708580929430504	24.468753829126566	21.876852301062243	25.945812940380687
17	26.119250582027238	24.13253783799669	23.240544361085018	26.507667218891058
18	25.851971321523102	22.616427861995042	24.890543016221052	26.641057800260803
19	26.503881675879814	23.73744669108674	24.135924902796223	25.62274673023722
20	27.4213777583409	22.620113785453352	23.781279294374784	26.17722916183097
21	28.005148338495285	22.972069665945753	23.44536216190369	25.57741983365527
22	27.53763377660714	22.962107710653015	23.7598610904954	25.74039742224445
23	26.60150883774864	22.804609197474843	23.6222864879027	26.971595476873816
24	26.480471080941882	23.47225944119408	23.929712428236567	26.11755704962747
25	26.801246041368014	23.36686195419692	23.355106846951493	26.47678515748357
26	26.902260268036372	23.64161268117061	23.449745422232493	26.006381628560526
27	27.08336861525833	22.71186339369946	23.74651207040313	26.45825592063908
28	25.85695229916947	23.241440937061363	23.899328464593715	27.002278299175448
29	25.427193547840798	23.797118803290235	24.577936859134965	26.197750789734002
30	25.898493652740186	24.029730459375646	23.991675790157387	26.08010009772678
31	26.250549152785513	24.21850951217301	23.327811089449394	26.20313024559208
32	25.82925806345566	23.52565552156315	23.619795999079514	27.025290415901672
33	25.19896515208419	23.45183743284397	24.578634196005456	26.770563219066386
34	26.685587740419336	22.784386428230587	23.93140596063633	26.598619870713748
35	26.023416572111106	22.87782956887646	24.66600054392276	26.432753315089673
36	25.274974870967775	24.150070879311908	24.202271525045848	26.37268272467447
37	26.49880107868052	22.927240867128436	24.48668534865349	26.08727270553755
38	25.577021355443563	22.966590590534746	24.891140733538617	26.565247320483078
39	25.710611175919162	22.64402247815592	25.021443108767617	26.6239232371573
40	24.751772978993223	22.36976984894687	26.09603922619516	26.782417945864744
41	24.313048467901087	22.685364592620783	26.41950391455033	26.5820830249278
42	25.046049138340678	22.45195598011195	26.672736818091707	25.82925806345566
43	24.438569104589572	22.00506266567977	26.429665108948925	27.126703120781738
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	25.0
1	24.5
2	24.0
3	101.0
4	178.0
5	178.0
6	233.5
7	289.0
8	306.0
9	323.0
10	421.5
11	520.0
12	520.0
13	946.5
14	1373.0
15	1954.5
16	2536.0
17	2554.0
18	2572.0
19	2572.0
20	3346.5
21	4121.0
22	4268.0
23	4415.0
24	5563.0
25	6711.0
26	6711.0
27	8046.0
28	9381.0
29	11446.0
30	13511.0
31	15995.0
32	18479.0
33	18479.0
34	21891.5
35	25304.0
36	28815.0
37	32326.0
38	35730.5
39	39135.0
40	39135.0
41	42072.0
42	45009.0
43	47430.5
44	49852.0
45	53303.5
46	56755.0
47	56755.0
48	61766.0
49	66777.0
50	68069.5
51	69362.0
52	73668.5
53	77975.0
54	77975.0
55	77012.0
56	76049.0
57	76701.5
58	77354.0
59	76456.0
60	75558.0
61	75558.0
62	72079.5
63	68601.0
64	62859.0
65	57117.0
66	50568.0
67	44019.0
68	44019.0
69	37426.0
70	30833.0
71	26055.5
72	21278.0
73	16197.0
74	11116.0
75	11116.0
76	8549.0
77	5982.0
78	4823.5
79	3665.0
80	3139.5
81	2614.0
82	2614.0
83	1878.5
84	1143.0
85	964.5
86	786.0
87	602.0
88	418.0
89	418.0
90	345.0
91	272.0
92	153.5
93	35.0
94	25.5
95	16.0
96	16.0
97	11.0
98	6.0
99	5.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1003819.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.30957621405979
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.19539281574824	57.36117912227362
2	7.140191827569661	9.183654210361961
3	1.485106297548286	2.8651966988448496
4	0.57318620767415	1.4744544842907459
5	0.315824661488988	1.0155275069152854
6	0.1905228422998348	0.7351465948440312
7	0.13494526714365326	0.6074791059470999
8	0.08079518958565908	0.41567235219106813
9	0.07861412871727187	0.4550077172020771
>10	0.6074420904734822	8.181424680127144
>50	0.10233689263571448	4.543297023544838
>100	0.09067337526050497	10.800837508666413
>500	0.004502615993231932	1.9716130864439667
>1k	4.657878613688206E-4	0.38950990834690136
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1408	0.14026433052173748	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1351	0.13458601600487735	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	1142	0.11376552944305696	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	9.961955292737037E-4	0.0
3	0.0	0.0	0.0	0.0022912497173295187	0.0
4	0.0	0.0	0.0	0.004184021222949556	0.0
5	0.0	0.0	0.0	0.0046821189875864075	0.0
6	0.0	0.0	0.0	0.0046821189875864075	0.0
7	0.0	0.0	0.0	0.00547907541100537	0.0
8	0.0	0.0	0.0	0.0057779340697874815	0.0
9	0.0	0.0	0.0	0.009364237975172815	0.0
10	0.0	0.0	0.0	0.014843313386178185	0.0
11	0.0	0.0	0.0	0.017034943550580335	0.0
12	0.0	0.0	0.0	0.018031139079854038	0.0
13	0.0	0.0	0.0	0.01902733460912774	0.0
14	0.0	0.0	0.0	0.019923910585474075	0.0
15	0.0	0.0	0.0	0.02141820387938463	0.0
16	0.0	0.0	0.0	0.023510214490859407	0.0
17	0.0	0.0	0.0	0.027096518396244742	0.0
18	0.0	1.9923910585474075E-4	0.0	0.028192333478445814	0.0
19	0.0	1.9923910585474075E-4	0.0	0.029985485431138482	0.0
20	0.0	1.9923910585474075E-4	0.0	0.03187825693675852	9.961955292737038E-5
21	0.0	1.9923910585474075E-4	0.0	0.035265321736289115	9.961955292737038E-5
22	0.0	1.9923910585474075E-4	0.0	0.03994744072387552	9.961955292737038E-5
23	0.0	2.988586587821111E-4	0.0	0.046522331217081964	9.961955292737038E-5
24	0.0	3.984782117094815E-4	0.0	0.05877553622714852	9.961955292737038E-5
25	0.0	3.984782117094815E-4	0.0	0.06295955745009807	9.961955292737038E-5
26	0.0	3.984782117094815E-4	0.0	0.06853825241403082	9.961955292737038E-5
27	0.0	3.984782117094815E-4	0.0	0.07999450100067841	9.961955292737038E-5
28	0.0	3.984782117094815E-4	0.0	0.10460053057373889	9.961955292737038E-5
29	0.0	3.984782117094815E-4	0.0	0.14185824336857542	9.961955292737038E-5
30	0.0	3.984782117094815E-4	0.0	0.19764519300790281	9.961955292737038E-5
31	0.0	3.984782117094815E-4	0.0	0.36361136818490186	9.961955292737038E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCTAAT	45	0.003825476	20.555555	1
ATACCGT	135	1.8189894E-12	20.555555	6
CCTATAG	65	6.901015E-5	19.923077	3
GGTATCA	1110	0.0	19.166666	1
TACCGTC	145	7.2759576E-12	19.13793	7
CTTACAC	120	5.171387E-9	18.5	3
CTGTACG	50	0.007034278	18.499998	4
TAGCGAG	50	0.007034278	18.499998	37
CTAGCGG	180	0.0	17.472221	29
GTATTAA	160	3.45608E-11	17.34375	1
CGTCGTA	150	2.5102054E-10	17.266666	10
GTCCTAT	195	0.0	17.076923	1
ATTCCGC	120	1.04078936E-7	16.958332	28
ACCGTCG	155	4.0017767E-10	16.709677	8
GTATAGA	90	4.4450542E-5	16.444445	1
CTATGCT	80	3.3820313E-4	16.1875	4
TCGATGC	160	6.2937033E-10	16.1875	29
ATTTTCG	80	3.3820313E-4	16.1875	15
CGCAATA	185	1.8189894E-11	16.0	36
CTAGACT	105	9.340658E-6	15.857144	4
>>END_MODULE