FastQCFastQC Report
Fri 10 Feb 2017
ERR1630627.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630627.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1447772
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT69070.4770778824290013No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT61860.4272772232091793No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT56480.3901166758301722No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40560.28015460998002445No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC24960.17240283691078429No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA21090.14567210859168434No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT21080.14560303694228097No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA20660.1427020276673399No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG16830.11624758594585335No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCCCG400.001931428223.1255
CTAGTAC656.9037116E-519.9230773
GCTTTAT7850.018.8535041
GAACCGT500.00703574418.4999986
GGTATCA35600.017.928371
TATTGAG7600.017.7697355
GTATTAC752.0677333E-417.2666661
GGCGATA650.001580201717.07692335
TTTATTG9100.016.670333
GTATAGA1504.6729838E-916.0333331
CGATAGA700.002592929915.85714237
TATACTG1659.749783E-1015.696975
TAGCACC957.061701E-515.5789464
AAGACGG2500.015.545
GTATAGG1353.9739098E-715.0740731
TAGGCCG750.004105089714.85
GTAAGGC750.004105089714.83
TCGGTGA2251.8189894E-1214.813
TCTATCC750.004105089714.83
CTTTATT10150.014.3990142
Produced by FastQC (version 0.11.5)