##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630627.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1447772
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.94582157964099	33.0	31.0	34.0	30.0	34.0
2	32.10056348651583	33.0	31.0	34.0	30.0	34.0
3	32.21447990429432	34.0	31.0	34.0	30.0	34.0
4	35.80331778760744	37.0	35.0	37.0	35.0	37.0
5	35.78227165603424	37.0	35.0	37.0	35.0	37.0
6	35.81946259493898	37.0	35.0	37.0	35.0	37.0
7	35.79573648336893	37.0	35.0	37.0	35.0	37.0
8	35.7948019439525	37.0	35.0	37.0	35.0	37.0
9	37.50067759288065	39.0	37.0	39.0	35.0	39.0
10	37.415142715841995	39.0	37.0	39.0	34.0	39.0
11	37.51925717585365	39.0	37.0	39.0	35.0	39.0
12	37.45312245298293	39.0	37.0	39.0	35.0	39.0
13	37.50764968517142	39.0	37.0	39.0	35.0	39.0
14	38.75766280878481	40.0	38.0	41.0	35.0	41.0
15	38.73259877936581	40.0	38.0	41.0	35.0	41.0
16	38.70145230050036	40.0	38.0	41.0	35.0	41.0
17	38.706451015767676	40.0	38.0	41.0	35.0	41.0
18	38.70524985978455	40.0	38.0	41.0	35.0	41.0
19	38.7297979239825	40.0	38.0	41.0	34.0	41.0
20	38.71582956432366	40.0	38.0	41.0	34.0	41.0
21	38.63834015300752	40.0	38.0	41.0	34.0	41.0
22	38.579641683911554	40.0	38.0	41.0	34.0	41.0
23	38.55968066795048	40.0	38.0	41.0	34.0	41.0
24	38.507575087790066	40.0	38.0	41.0	34.0	41.0
25	38.46654998162694	40.0	38.0	41.0	34.0	41.0
26	38.26293228491779	40.0	38.0	41.0	34.0	41.0
27	38.14064023893265	40.0	38.0	41.0	33.0	41.0
28	38.02911439094001	40.0	37.0	41.0	33.0	41.0
29	37.92780631204361	40.0	37.0	41.0	33.0	41.0
30	37.84835043086895	40.0	37.0	41.0	33.0	41.0
31	37.79384322945878	40.0	37.0	41.0	33.0	41.0
32	37.69515365679126	40.0	37.0	41.0	33.0	41.0
33	37.647859607728286	40.0	37.0	41.0	33.0	41.0
34	37.56464968240856	40.0	37.0	41.0	33.0	41.0
35	37.494805121248376	40.0	36.0	41.0	32.0	41.0
36	37.441357478940056	40.0	36.0	41.0	32.0	41.0
37	37.24545646690225	40.0	36.0	41.0	31.0	41.0
38	37.12672022942839	40.0	36.0	41.0	31.0	41.0
39	37.04257714612522	39.0	36.0	41.0	31.0	41.0
40	36.911446691882425	39.0	35.0	41.0	31.0	41.0
41	36.774190963770536	39.0	35.0	41.0	30.0	41.0
42	36.66110064291891	39.0	35.0	41.0	30.0	41.0
43	35.831448598259946	38.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	6.0
10	3.0
11	3.0
12	3.0
13	4.0
14	2.0
15	4.0
16	8.0
17	24.0
18	39.0
19	97.0
20	166.0
21	414.0
22	781.0
23	1381.0
24	2202.0
25	3632.0
26	5548.0
27	8246.0
28	11898.0
29	16571.0
30	22356.0
31	29341.0
32	37269.0
33	48339.0
34	64258.0
35	84509.0
36	118209.0
37	181862.0
38	321665.0
39	488932.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.10791201929586	19.574698225963758	12.199227502673073	24.118162252067314
2	18.993184009636877	21.251550658529105	33.636511826447816	26.118753505386206
3	21.307705909494036	23.858729136908295	28.542892112846495	26.290672840751167
4	14.538131694769618	15.078479208052098	34.41253180749455	35.970857289683735
5	14.386795710926858	37.36990354834877	33.75414084538173	14.489159895342638
6	35.085082457735055	34.13168648102049	15.44559502463095	15.3376360366135
7	28.913185225297905	30.561718281607874	21.51733836543323	19.007758127660985
8	28.490812089196364	31.536457397988084	20.16263610568515	19.810094407130403
9	26.870736552440576	13.867100620815984	20.57540828251962	38.68675454422382
10	18.11238233644524	26.722163434573954	31.97713452118151	23.188319707799295
11	35.059042445909995	22.287901686177104	21.314682146083776	21.33837372182913
12	22.919354705022617	26.121861729609357	27.960341821778567	22.99844174358946
13	30.17028924443904	19.966748907977223	25.59968006011996	24.26328178746377
14	22.968671862696613	21.30984713062554	25.724354387293026	29.997126619384822
15	25.967486593192852	27.74221355296276	22.959485333325965	23.330814520518427
16	24.909999640827422	26.304625313930647	24.868902009432425	23.916473035809503
17	23.761199967950756	26.45022835087293	25.620470626590375	24.168101054585943
18	23.119109915097127	24.76722854151068	28.059183352074772	24.054478191317415
19	24.373589211560937	25.12605576016113	27.839052005426268	22.661303022851666
20	24.960214729943665	24.457856623833035	27.003077832697414	23.578850813525886
21	24.965947676844145	24.858955691918343	26.98594806364538	23.189148567592134
22	24.874082383137676	25.09524980452723	27.06538046045924	22.965287351875848
23	24.07409453974797	24.887413211472527	26.95224109873654	24.086251150042962
24	23.500523563102476	25.7443160939706	27.24234202623065	23.512818316696276
25	23.99984251663936	25.658114675515204	26.64535576043742	23.696687047408016
26	23.734054809735234	26.15619033936283	26.916945485891425	23.192809365010515
27	24.187855546315305	24.962079664477557	26.942156637923652	23.90790815128349
28	23.285848876756837	25.644714775530954	27.273769626709175	23.79566672100303
29	23.4084510544478	26.083665107489303	27.496318481086803	23.0115653569761
30	23.493685469811545	26.035038666309333	26.90796617146899	23.56330969241013
31	22.885026095269147	26.67077412741785	27.108135811439922	23.33606396587308
32	22.803038047427357	25.840463829940074	26.861688166368737	24.494809956263833
33	22.222283619243914	26.26988227428076	27.510547240863893	23.997286865611436
34	24.1528362200678	25.125986688511727	27.018688025462573	23.7024890659579
35	23.196953663974714	25.361797299574796	27.314867258104176	24.126381778346314
36	22.42645941488024	26.302000591253318	27.44803739815385	23.823502595712583
37	23.21208035519405	24.956484860875882	27.91406381667832	23.91737096725175
38	23.13472010786229	25.03004616749046	27.77488444313055	24.0603492815167
39	22.927159801405196	24.900951254755583	28.02734132169983	24.1445476221394
40	22.169858237346766	24.828978596077285	28.53232415048779	24.468839016088168
41	21.308949199183296	25.187184169883103	29.161359661604173	24.342506969329424
42	22.07930530497896	25.055948036016723	29.25591874963737	23.608827909366944
43	22.156043907466092	24.738563807008283	28.73670716107232	24.3686851244533
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	611.0
1	546.0
2	481.0
3	1040.0
4	1599.0
5	1599.0
6	2189.0
7	2779.0
8	2540.0
9	2301.0
10	3378.0
11	4455.0
12	4455.0
13	7541.0
14	10627.0
15	13721.5
16	16816.0
17	16590.0
18	16364.0
19	16364.0
20	18669.0
21	20974.0
22	19665.5
23	18357.0
24	20513.0
25	22669.0
26	22669.0
27	25397.5
28	28126.0
29	31607.0
30	35088.0
31	39060.5
32	43033.0
33	43033.0
34	48033.5
35	53034.0
36	57760.0
37	62486.0
38	67619.0
39	72752.0
40	72752.0
41	75213.0
42	77674.0
43	79008.0
44	80342.0
45	82291.0
46	84240.0
47	84240.0
48	89271.0
49	94302.0
50	93098.5
51	91895.0
52	91700.0
53	91505.0
54	91505.0
55	88906.5
56	86308.0
57	84414.5
58	82521.0
59	84068.5
60	85616.0
61	85616.0
62	79464.5
63	73313.0
64	67095.0
65	60877.0
66	52670.0
67	44463.0
68	44463.0
69	39618.0
70	34773.0
71	29274.0
72	23775.0
73	17364.5
74	10954.0
75	10954.0
76	7812.0
77	4670.0
78	3748.5
79	2827.0
80	2375.5
81	1924.0
82	1924.0
83	1516.0
84	1108.0
85	1008.0
86	908.0
87	820.0
88	732.0
89	732.0
90	570.5
91	409.0
92	235.0
93	61.0
94	40.5
95	20.0
96	20.0
97	11.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1447772.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.92491503813568
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.89032163367571	50.60074006709545
2	6.867210449767834	7.81830742804063
3	1.5803801979715844	2.6988902549245344
4	0.6787887298928766	1.5455996311198414
5	0.36966008651722465	1.0521434508991452
6	0.23668578432128645	0.8083990897934242
7	0.16420208643953438	0.6543032873758575
8	0.13071427400091587	0.5952719153418979
9	0.09665629799438492	0.49519363961079776
>10	0.7456121318849844	8.583095166165327
>50	0.11314920028653863	4.5443467387546175
>100	0.11348287561871413	13.209134397472672
>500	0.010217084599954188	3.8786241186120325
>1k	0.002554271149988547	2.218344784465902
>5k	3.6489587856979245E-4	1.2976060303278965
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6907	0.4770778824290013	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6186	0.4272772232091793	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5648	0.3901166758301722	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4056	0.28015460998002445	No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC	2496	0.17240283691078429	No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA	2109	0.14567210859168434	No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT	2108	0.14560303694228097	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	2066	0.1427020276673399	No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG	1683	0.11624758594585335	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	6.90716494033591E-5	0.0
2	0.0	0.0	0.0	8.979314422436682E-4	0.0
3	0.0	6.90716494033591E-5	0.0	0.0011742180398571045	0.0
4	0.0	1.381432988067182E-4	0.0	0.002210292780907491	0.0
5	6.90716494033591E-5	1.381432988067182E-4	0.0	0.0025556510279242865	0.0
6	6.90716494033591E-5	1.381432988067182E-4	0.0	0.0025556510279242865	0.0
7	6.90716494033591E-5	1.381432988067182E-4	0.0	0.0034535824701679547	0.0
8	6.90716494033591E-5	1.381432988067182E-4	0.0	0.00379894071718475	0.0
9	6.90716494033591E-5	1.381432988067182E-4	0.0	0.0059401618486888816	0.0
10	6.90716494033591E-5	1.381432988067182E-4	0.0	0.0091174577212434	0.0
11	6.90716494033591E-5	1.381432988067182E-4	0.0	0.010913320605730737	0.0
12	6.90716494033591E-5	1.381432988067182E-4	0.0	0.012501968542007996	0.0
13	6.90716494033591E-5	1.381432988067182E-4	0.0	0.013814329880671819	0.0
14	6.90716494033591E-5	1.381432988067182E-4	0.0	0.015057619569932281	0.0
15	1.381432988067182E-4	1.381432988067182E-4	0.0	0.017060697402629697	0.0
16	1.381432988067182E-4	1.381432988067182E-4	0.0	0.02134313966563796	0.0
17	1.381432988067182E-4	1.381432988067182E-4	0.0	0.025763725227452943	0.0
18	1.381432988067182E-4	1.381432988067182E-4	0.0	0.027974018008360434	0.0
19	2.762865976134364E-4	1.381432988067182E-4	0.0	0.03170388707614182	0.0
20	2.762865976134364E-4	1.381432988067182E-4	0.0	0.03432860975346947	0.0
21	4.1442989642015454E-4	1.381432988067182E-4	0.0	0.03992341335514155	0.0
22	4.8350154582351367E-4	1.381432988067182E-4	0.0	0.050560447363258855	0.0
23	4.8350154582351367E-4	1.381432988067182E-4	0.0	0.0653417803355777	0.0
24	4.8350154582351367E-4	1.381432988067182E-4	0.0	0.09013850247138362	0.0
25	4.8350154582351367E-4	1.381432988067182E-4	0.0	0.09628587926828258	0.0
26	4.8350154582351367E-4	1.381432988067182E-4	0.0	0.10906413440790401	0.0
27	4.8350154582351367E-4	1.381432988067182E-4	0.0	0.13158149211339906	0.0
28	6.216448446302318E-4	1.381432988067182E-4	0.0	0.18041514824157395	0.0
29	6.216448446302318E-4	1.381432988067182E-4	0.0	0.24865793785209273	0.0
30	6.216448446302318E-4	1.381432988067182E-4	0.0	0.3429407392876779	0.0
31	6.216448446302318E-4	1.381432988067182E-4	0.0	0.6347684580168701	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCCCG	40	0.0019314282	23.125	5
CTAGTAC	65	6.9037116E-5	19.923077	3
GCTTTAT	785	0.0	18.853504	1
GAACCGT	50	0.007035744	18.499998	6
GGTATCA	3560	0.0	17.92837	1
TATTGAG	760	0.0	17.769735	5
GTATTAC	75	2.0677333E-4	17.266666	1
GGCGATA	65	0.0015802017	17.076923	35
TTTATTG	910	0.0	16.67033	3
GTATAGA	150	4.6729838E-9	16.033333	1
CGATAGA	70	0.0025929299	15.857142	37
TATACTG	165	9.749783E-10	15.69697	5
TAGCACC	95	7.061701E-5	15.578946	4
AAGACGG	250	0.0	15.54	5
GTATAGG	135	3.9739098E-7	15.074073	1
TAGGCCG	75	0.0041050897	14.8	5
GTAAGGC	75	0.0041050897	14.8	3
TCGGTGA	225	1.8189894E-12	14.8	13
TCTATCC	75	0.0041050897	14.8	3
CTTTATT	1015	0.0	14.399014	2
>>END_MODULE