FastQCFastQC Report
Fri 10 Feb 2017
ERR1630626.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630626.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences269588
Sequences flagged as poor quality0
Sequence length43
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT132104.900069736041663No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT120764.479427867709245No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT95733.550974078964939No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63012.3372702049052627No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT17370.6443165126044186No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16050.5953529088831847No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14950.5545499057821565No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA14290.5300681039215396No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGT13980.5185690757748861No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11910.44178524266658753No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA11300.4191581227651083No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT10520.3902250842025609No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT10010.37130732821935697RNA PCR Primer, Index 26 (100% over 22bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG9650.3579536181135659No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA8080.29971660459664373No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7920.2937816223274033No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA7410.2748638663441993No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6900.2559461103609953No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA6590.24444708221434186No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA6300.23368992685134354No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6040.22404558066382776No Hit
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC5980.2218199623128626No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA5930.21996528035372495No Hit
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCC5880.21811059839458732No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA5710.2118046797335193No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT5290.19622535127676305No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA5220.19362879653397036No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATG5170.1917741145748327No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG5060.1876938142647299No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA5020.18621006869741977No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA4790.1776785316853866No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA4670.17322729498345624No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA4100.15208392064928705No Hit
GAGTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAA3780.14021395611080611No Hit
GTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAA3630.13464991023339318No Hit
GTACATGGGAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAA3570.13242429188242802No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT3550.13168241909877293No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC3550.13168241909877293No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC3510.1301986735314628No Hit
GTACATGGGAACATGGTATCAACGCAAAAAAAAAAAAAAAAAA3370.1250055640458774No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA3240.12018339095211954No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3150.11684496342567177No Hit
ACGCAGAGTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAA3140.11647402703384424No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA3110.11536121785836165No Hit
ACGCAGAGTACATGGGAACAGTGGTATCAACGCAAAAAAAAAA3010.11165185394008635No Hit
GAACAGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAA2950.10942623558912118No Hit
GTACATGGGAAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA2930.10868436280546612No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA2870.10645874445450094No Hit
GAGTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAA2860.10608780806267341No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAA2790.1034912533198807No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC2750.1020075077525706No Hit
GGTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATA2720.100894698577088No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT250.00548897529.5999986
TACACTG250.00548897529.5999985
CCGTCTT1500.028.36666537
CATGGAG358.8517164E-426.4285724
GTTATAC358.8517164E-426.4285723
GCCGTCT1650.025.78787836
CGGAACT400.001927375423.1254
TTTGTAG400.001927375423.1252
GACCGTT400.001927375423.1257
TACGGGT400.001927375423.1254
ATGCCCC400.001927375423.12537
CGTTGAA450.003818328720.5555552
CGTCTTC450.003818328720.55555537
CGACAAG450.003818328720.55555515
GGAATGT1202.3646862E-1020.0416668
AGTGGCT609.211834E-418.527
TTTGGGG902.1398064E-618.537
GGGCTTC500.00702126118.49999837
CCGACAA500.00702126118.49999814
TGCCGTC2400.017.72916635
Produced by FastQC (version 0.11.5)