##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630626.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	269588
Sequences flagged as poor quality	0
Sequence length	43
%GC	33
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.862323248809293	33.0	31.0	34.0	30.0	34.0
2	31.85235618796089	33.0	31.0	34.0	30.0	34.0
3	31.783536359185128	33.0	31.0	34.0	28.0	34.0
4	35.532642402480825	37.0	35.0	37.0	33.0	37.0
5	35.585994183717375	37.0	35.0	37.0	33.0	37.0
6	35.73965087466801	37.0	35.0	37.0	35.0	37.0
7	35.641738504681214	37.0	35.0	37.0	33.0	37.0
8	35.780275828300965	37.0	35.0	37.0	35.0	37.0
9	37.28019422229476	39.0	37.0	39.0	34.0	39.0
10	37.24203970503138	39.0	37.0	39.0	34.0	39.0
11	37.43767526744514	39.0	37.0	39.0	34.0	39.0
12	37.43402154398564	39.0	37.0	39.0	35.0	39.0
13	37.503412614804816	39.0	37.0	39.0	35.0	39.0
14	38.46711648886449	40.0	38.0	41.0	34.0	41.0
15	38.35542754128522	40.0	38.0	41.0	34.0	41.0
16	38.16171713874505	40.0	37.0	41.0	33.0	41.0
17	38.45517975577548	40.0	38.0	41.0	34.0	41.0
18	38.618458536730124	40.0	38.0	41.0	34.0	41.0
19	38.75881715803374	40.0	38.0	41.0	35.0	41.0
20	38.82851610605813	40.0	38.0	41.0	35.0	41.0
21	38.78417066041515	40.0	38.0	41.0	35.0	41.0
22	38.654176001899195	40.0	38.0	41.0	35.0	41.0
23	38.55270635191477	40.0	38.0	41.0	35.0	41.0
24	38.409639894950814	40.0	38.0	41.0	34.0	41.0
25	38.30116696588869	40.0	37.0	41.0	34.0	41.0
26	37.869248631244716	40.0	36.0	41.0	33.0	41.0
27	37.54290621244269	39.0	35.0	41.0	33.0	41.0
28	37.34191432853094	39.0	35.0	41.0	33.0	41.0
29	37.000571242043414	39.0	35.0	41.0	33.0	41.0
30	36.623566330845584	38.0	35.0	40.0	32.0	41.0
31	36.13969093579833	37.0	35.0	40.0	31.0	41.0
32	35.66884653619597	37.0	35.0	40.0	31.0	41.0
33	35.27941896523584	35.0	35.0	40.0	30.0	41.0
34	34.89756962476075	35.0	35.0	40.0	28.0	41.0
35	34.49357167232963	35.0	34.0	40.0	25.0	41.0
36	34.03230485036426	35.0	34.0	40.0	22.0	41.0
37	33.40904268735997	35.0	33.0	40.0	18.0	41.0
38	32.85011944151817	35.0	33.0	40.0	15.0	41.0
39	32.260460406249535	35.0	33.0	39.0	10.0	41.0
40	31.5981979910085	35.0	32.0	39.0	10.0	41.0
41	30.866058578275	35.0	30.0	39.0	7.0	41.0
42	30.150611303173733	35.0	25.0	39.0	7.0	41.0
43	29.195943439618976	35.0	22.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	1.0
11	7.0
12	3.0
13	6.0
14	2.0
15	4.0
16	2.0
17	6.0
18	21.0
19	40.0
20	91.0
21	192.0
22	369.0
23	628.0
24	1053.0
25	1671.0
26	2496.0
27	3708.0
28	5370.0
29	6837.0
30	8489.0
31	9558.0
32	11501.0
33	14130.0
34	17221.0
35	20525.0
36	28086.0
37	51923.0
38	46475.0
39	39171.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	52.82356781459116	19.410730447942786	14.432764069617344	13.332937667848718
2	16.439900885796106	27.412199356054423	32.99034081635681	23.15755894179266
3	25.33198807068564	31.497692775642832	30.565158686588422	12.605160467083104
4	12.770227161446353	18.571672329628917	28.140347493211866	40.51775301571286
5	9.226300873926139	47.75175452913334	28.127364719497898	14.894579877442615
6	28.072837069899254	28.74757036663353	20.198970280576287	22.98062228289093
7	21.52321319940057	40.183168390284436	21.79770612935294	16.49591228096206
8	36.42892116859801	27.52385120999451	19.818389542561242	16.228838078846238
9	30.1363562176358	9.92588690891286	24.752585426651038	35.185171446800304
10	21.64265471756903	34.56311111770554	23.824873510690388	19.969360654035047
11	23.930219445969406	27.691514459100553	18.62916747036218	29.74909862456786
12	20.19266436191522	36.75311957505527	28.697123017344982	14.357093045684527
13	30.904565485110613	20.401872486905944	32.76852085404395	15.92504117393949
14	24.009970770212323	24.949923587103285	35.29014644568749	15.7499591969969
15	29.0988471296942	22.96244639969138	33.29821802157366	14.64048844904076
16	17.850942920308025	24.80451652150689	38.31772927578379	19.026811282401294
17	14.986201166224017	32.21248720269448	35.352834695906346	17.448476935175155
18	13.668264166060803	22.29772838553645	42.23370476430702	21.800302684095733
19	14.571865216552665	27.53609211092482	46.09144323931332	11.800599433209193
20	16.09641378696381	22.27250471089218	47.30032494027924	14.33075656186477
21	20.072851907354927	22.627119901479293	43.97079988723534	13.329228303930444
22	18.34688487618143	24.606065551879162	42.86874786711575	14.178301704823657
23	16.207323768120244	26.180690535187022	42.560870661898896	15.051115034793833
24	13.7558051545321	28.884445895217887	44.07985518643263	13.27989376381738
25	13.62078430790688	28.210454471267266	43.09724468448151	15.071516536344348
26	14.972476519726396	27.633648381975455	43.23486208584952	14.159013012448627
27	12.635577251212963	30.223897206107097	42.99115687641883	14.149368666261108
28	12.69121770998709	29.647832989598943	44.54908972209445	13.11185957831951
29	12.204549163909373	30.670133685475616	44.939685742688845	12.185631407926168
30	11.659643604314732	32.01663278780955	44.42630977639954	11.897413831476179
31	11.388860038280637	32.85977120643352	42.439574461771294	13.311794293514549
32	10.427763847055507	33.84905856343754	43.8328115494755	11.890366040031456
33	10.894772764366365	33.60201492648041	43.473003249402794	12.030209059750433
34	11.013472409751175	35.36247904209387	40.7473626422541	12.876685905900857
35	11.00234431799635	35.05423090048518	41.130168998620114	12.813255782898347
36	10.512708280784011	35.56983248512545	39.41941035951155	14.498048874578986
37	10.726738578868495	36.27572443877324	39.50917696633381	13.488360016024453
38	10.80278053919314	34.713711292787515	39.94947846343309	14.534029704586256
39	11.631823374927668	35.112838850393935	39.39233200290814	13.863005771770256
40	10.722287342166565	35.32315978456014	39.8552606199089	14.099292253364393
41	10.372123388281377	36.62588839265843	37.97720966808612	15.02477855097408
42	10.698918349481431	36.03721233882814	38.498004362211965	14.765864949478463
43	9.885454842203659	37.23644969360654	37.60033829398935	15.277757170200454
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	16.0
1	95.0
2	174.0
3	783.0
4	1392.0
5	1392.0
6	2264.5
7	3137.0
8	3261.0
9	3385.0
10	4588.0
11	5791.0
12	5791.0
13	10485.0
14	15179.0
15	21549.5
16	27920.0
17	24627.0
18	21334.0
19	21334.0
20	22899.0
21	24464.0
22	17028.5
23	9593.0
24	8655.5
25	7718.0
26	7718.0
27	8101.0
28	8484.0
29	8450.0
30	8416.0
31	8182.0
32	7948.0
33	7948.0
34	7563.5
35	7179.0
36	6893.0
37	6607.0
38	7138.5
39	7670.0
40	7670.0
41	8382.0
42	9094.0
43	9568.5
44	10043.0
45	11909.0
46	13775.0
47	13775.0
48	15249.5
49	16724.0
50	14499.5
51	12275.0
52	10543.0
53	8811.0
54	8811.0
55	7100.5
56	5390.0
57	5977.0
58	6564.0
59	5779.5
60	4995.0
61	4995.0
62	4245.0
63	3495.0
64	3277.5
65	3060.0
66	2873.0
67	2686.0
68	2686.0
69	2447.0
70	2208.0
71	1886.5
72	1565.0
73	1318.0
74	1071.0
75	1071.0
76	873.0
77	675.0
78	545.0
79	415.0
80	317.5
81	220.0
82	220.0
83	149.0
84	78.0
85	50.5
86	23.0
87	17.5
88	12.0
89	12.0
90	6.5
91	1.0
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	269588.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.56839325192516
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.6074007952609	29.47683131296645
2	9.6871703318997	7.084885083905812
3	3.1607563093402584	3.4675133908037448
4	1.6463117747301792	2.408119055744321
5	0.9768319402742839	1.7860587266495542
6	0.6319483891909438	1.386560232651305
7	0.4767507912034407	1.2203807291125717
8	0.3671995455652033	1.0742317907325252
9	0.27286375071005436	0.8980370046144487
>10	1.8116530065730747	12.944567265605295
>50	0.18461413616814087	4.671943855067733
>100	0.14606832751764992	9.836120302090597
>500	0.017244177554167003	4.011677077614731
>1k	0.00912927046985312	4.465332284819799
>5k	0.002028726771078471	5.888244283870201
>10k+	0.002028726771078471	9.379497603750908
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	13210	4.900069736041663	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	12076	4.479427867709245	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	9573	3.550974078964939	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6301	2.3372702049052627	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1737	0.6443165126044186	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1605	0.5953529088831847	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1495	0.5545499057821565	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	1429	0.5300681039215396	No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGT	1398	0.5185690757748861	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1191	0.44178524266658753	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	1130	0.4191581227651083	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	1052	0.3902250842025609	No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT	1001	0.37130732821935697	RNA PCR Primer, Index 26 (100% over 22bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	965	0.3579536181135659	No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	808	0.29971660459664373	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	792	0.2937816223274033	No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA	741	0.2748638663441993	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	690	0.2559461103609953	No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	659	0.24444708221434186	No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA	630	0.23368992685134354	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	604	0.22404558066382776	No Hit
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC	598	0.2218199623128626	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	593	0.21996528035372495	No Hit
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCC	588	0.21811059839458732	No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	571	0.2118046797335193	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT	529	0.19622535127676305	No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA	522	0.19362879653397036	No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATG	517	0.1917741145748327	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	506	0.1876938142647299	No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA	502	0.18621006869741977	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	479	0.1776785316853866	No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA	467	0.17322729498345624	No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA	410	0.15208392064928705	No Hit
GAGTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAA	378	0.14021395611080611	No Hit
GTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAA	363	0.13464991023339318	No Hit
GTACATGGGAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	357	0.13242429188242802	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT	355	0.13168241909877293	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	355	0.13168241909877293	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC	351	0.1301986735314628	No Hit
GTACATGGGAACATGGTATCAACGCAAAAAAAAAAAAAAAAAA	337	0.1250055640458774	No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA	324	0.12018339095211954	No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	315	0.11684496342567177	No Hit
ACGCAGAGTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAA	314	0.11647402703384424	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	311	0.11536121785836165	No Hit
ACGCAGAGTACATGGGAACAGTGGTATCAACGCAAAAAAAAAA	301	0.11165185394008635	No Hit
GAACAGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAA	295	0.10942623558912118	No Hit
GTACATGGGAAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	293	0.10868436280546612	No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA	287	0.10645874445450094	No Hit
GAGTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAA	286	0.10608780806267341	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAA	279	0.1034912533198807	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC	275	0.1020075077525706	No Hit
GGTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATA	272	0.100894698577088	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	7.418727836550588E-4	0.0
2	0.0	3.709363918275294E-4	0.0	0.0048221730937578824	0.0
3	0.0	3.709363918275294E-4	0.0	0.008160600620205648	0.0
4	0.0	3.709363918275294E-4	0.0	0.011499028146653411	0.0
5	0.0	3.709363918275294E-4	0.0	0.01335371010579106	0.0
6	0.0	3.709363918275294E-4	0.0	0.01335371010579106	0.0
7	0.0	3.709363918275294E-4	0.0	0.01335371010579106	0.0
8	0.0	3.709363918275294E-4	0.0	0.013724646497618589	0.0
9	0.0	3.709363918275294E-4	0.0	0.021514310725996705	0.0
10	0.0	3.709363918275294E-4	0.0	0.029674911346202353	0.0
11	0.0	3.709363918275294E-4	0.0	0.033013338872650115	0.0
12	0.0	3.709363918275294E-4	0.0	0.035609893615442825	0.0
13	0.0	3.709363918275294E-4	0.0	0.03709363918275294	0.0
14	0.0	3.709363918275294E-4	0.0	0.04377049423564847	0.0
15	0.0	3.709363918275294E-4	0.0	0.052672967639509176	0.0
16	0.0	3.709363918275294E-4	0.0	0.08420256094484918	0.0
17	0.0	3.709363918275294E-4	0.0	0.11869964538480941	0.0
18	0.0	3.709363918275294E-4	0.0	0.13835927415166846	0.0
19	0.0	3.709363918275294E-4	0.0	0.1665504399305607	0.0
20	0.0	3.709363918275294E-4	0.0	0.1813878956036619	0.0
21	0.0	3.709363918275294E-4	0.0	0.22998056293306823	0.0
22	0.0	3.709363918275294E-4	0.0	0.3230855972817781	0.0
23	0.0	3.709363918275294E-4	0.0	0.4655251717435494	0.0
24	0.0	3.709363918275294E-4	0.0	0.6784426606525513	0.0
25	0.0	3.709363918275294E-4	0.0	0.7233259640636823	0.0
26	0.0	3.709363918275294E-4	0.0	0.8112378889268068	0.0
27	0.0	3.709363918275294E-4	0.0	0.8650236657417986	0.0
28	0.0	3.709363918275294E-4	0.0	0.9243734884342033	0.0
29	0.0	3.709363918275294E-4	0.0	1.0111726041218452	0.0
30	0.0	3.709363918275294E-4	0.0	1.1777230440524058	0.0
31	0.0	3.709363918275294E-4	0.0	1.6562309895099188	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACCGT	25	0.005488975	29.599998	6
TACACTG	25	0.005488975	29.599998	5
CCGTCTT	150	0.0	28.366665	37
CATGGAG	35	8.8517164E-4	26.428572	4
GTTATAC	35	8.8517164E-4	26.428572	3
GCCGTCT	165	0.0	25.787878	36
CGGAACT	40	0.0019273754	23.125	4
TTTGTAG	40	0.0019273754	23.125	2
GACCGTT	40	0.0019273754	23.125	7
TACGGGT	40	0.0019273754	23.125	4
ATGCCCC	40	0.0019273754	23.125	37
CGTTGAA	45	0.0038183287	20.555555	2
CGTCTTC	45	0.0038183287	20.555555	37
CGACAAG	45	0.0038183287	20.555555	15
GGAATGT	120	2.3646862E-10	20.041666	8
AGTGGCT	60	9.211834E-4	18.5	27
TTTGGGG	90	2.1398064E-6	18.5	37
GGGCTTC	50	0.007021261	18.499998	37
CCGACAA	50	0.007021261	18.499998	14
TGCCGTC	240	0.0	17.729166	35
>>END_MODULE