FastQCFastQC Report
Fri 10 Feb 2017
ERR1630625.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630625.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences558740
Sequences flagged as poor quality0
Sequence length43
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT127882.2887210509360347No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT115732.0712674947202636No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT89261.597522998174464No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72241.2929090453520422No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20420.36546515373876937No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT19730.3531159394351577No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18800.3364713462433332No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT12790.2289078999176719No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12150.2174535562157712No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA9960.1782582238608297No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG9840.17611053441672334No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9430.16877259548269322No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG8330.14908544224505135No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7790.13942083974657266No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA7530.13476751261767547No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7230.12939828900740954No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7040.12599778072090775No Hit
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT6930.12402906539714359TruSeq Adapter, Index 12 (95% over 22bp)
GAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTG6570.11758599706482444No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG6530.11687010058345562No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG6500.11633317822242904No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGT6430.11508035938003365No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG6040.1081003686866879No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC5960.10666857572395033No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG5850.10469986040018613No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA5600.10022550739163118No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGC200.001841126237.06
GCACACT303.5971316E-430.8333346
TAAGTTC250.005493956629.5999983
GGAACGC405.9347483E-527.7500028
GCCGTCT1250.025.1636
GTCATGG601.3353529E-624.6666681
AAGGTCT451.3220233E-424.66666634
ACACTAC451.3220233E-424.6666668
CCGTCTT1400.023.78571337
CAGCGTC551.90039E-523.5454542
AGCGTCA551.90039E-523.5454543
GCAGCGT551.90039E-523.5454541
CTGACTT400.001929951523.12500231
AGGTGTC400.001929951523.12500214
TCGCGCG400.001929951523.1250029
TGGTAAT400.001929951523.12500234
TTGGTAA2050.022.56097633
GCCGAGT753.7336576E-722.19999912
GTAAACA2100.022.0238136
TAAACAG2100.021.14285737
Produced by FastQC (version 0.11.5)