##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630625.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	558740
Sequences flagged as poor quality	0
Sequence length	43
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.088932240398037	31.0	31.0	33.0	28.0	34.0
2	31.162746536850772	31.0	31.0	34.0	28.0	34.0
3	31.149203565164477	31.0	31.0	34.0	28.0	34.0
4	34.95560188996671	35.0	35.0	37.0	32.0	37.0
5	34.805739700039375	35.0	35.0	37.0	32.0	37.0
6	34.94256362529978	35.0	35.0	37.0	32.0	37.0
7	34.876346780255574	35.0	35.0	37.0	32.0	37.0
8	34.95834914271396	36.0	35.0	37.0	32.0	37.0
9	36.375362422593696	38.0	35.0	39.0	32.0	39.0
10	36.182236818556035	38.0	35.0	39.0	32.0	39.0
11	36.46724415649497	38.0	35.0	39.0	32.0	39.0
12	36.38356480652897	38.0	35.0	39.0	32.0	39.0
13	36.499176719046424	38.0	35.0	39.0	32.0	39.0
14	37.37328811253893	39.0	36.0	40.0	32.0	41.0
15	37.28364713462433	39.0	36.0	40.0	32.0	41.0
16	37.17736514300032	39.0	36.0	40.0	32.0	41.0
17	37.31117156459176	39.0	36.0	40.0	32.0	41.0
18	37.364693775280095	39.0	36.0	40.0	32.0	41.0
19	37.449810287432435	39.0	36.0	40.0	32.0	41.0
20	37.43768479077925	39.0	36.0	40.0	32.0	41.0
21	37.37199949887246	39.0	36.0	40.0	32.0	41.0
22	37.26530944625407	39.0	36.0	40.0	32.0	41.0
23	37.20488599348534	39.0	36.0	40.0	32.0	41.0
24	37.09055732541074	39.0	36.0	40.0	32.0	41.0
25	36.971206643519345	39.0	35.0	40.0	31.0	41.0
26	36.57588323728389	38.0	35.0	40.0	31.0	41.0
27	36.33124172244693	38.0	35.0	40.0	30.0	41.0
28	36.133009986755916	38.0	35.0	40.0	30.0	41.0
29	35.91881912875398	38.0	35.0	40.0	30.0	41.0
30	35.64755521351613	38.0	34.0	40.0	29.0	41.0
31	35.37884346923435	37.0	34.0	40.0	28.0	41.0
32	35.10820059419408	37.0	34.0	40.0	27.0	41.0
33	34.89183699037119	37.0	33.0	40.0	27.0	41.0
34	34.65677059097254	37.0	33.0	40.0	25.0	41.0
35	34.40786412284783	37.0	33.0	40.0	24.0	41.0
36	34.18421269284462	37.0	33.0	40.0	23.0	41.0
37	33.72364964026202	36.0	33.0	40.0	21.0	41.0
38	33.37114579231843	36.0	33.0	40.0	19.0	41.0
39	33.0424669792748	36.0	33.0	39.0	18.0	41.0
40	32.639308086050754	35.0	32.0	39.0	15.0	41.0
41	32.203892687117445	35.0	31.0	39.0	12.0	40.0
42	31.813072269749796	35.0	30.0	39.0	10.0	40.0
43	30.979824247413823	35.0	29.0	39.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	3.0
11	3.0
12	1.0
13	5.0
14	4.0
15	8.0
16	19.0
17	36.0
18	68.0
19	200.0
20	374.0
21	707.0
22	1193.0
23	1951.0
24	3013.0
25	4641.0
26	6561.0
27	9025.0
28	12036.0
29	15648.0
30	18900.0
31	22877.0
32	28006.0
33	34292.0
34	42997.0
35	55118.0
36	78060.0
37	99873.0
38	91903.0
39	31216.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.55242151984823	22.227690875899345	13.457422056770591	17.762465547481835
2	17.878977699824606	24.878476572287646	33.86601997351183	23.37652575437592
3	23.34341554211261	28.914521960124567	28.996492107241295	18.74557039052153
4	13.925976303826468	18.07799692164513	32.99924830869456	34.99677846583384
5	10.714106740165372	43.92132297669757	32.207824748541356	13.156745534595698
6	30.002863585925475	33.91541683072627	17.738125067115295	18.343594516232955
7	24.82335254322225	35.24286788130436	23.523105558936177	16.410674016537207
8	29.879013494648675	31.9912660629273	20.583455632315566	17.546264810108458
9	27.417045495221394	12.232523177148584	24.378422880051545	35.97200844757848
10	19.68625836704013	30.672226796005297	30.461037334001507	19.18047750295307
11	28.671654078820204	24.87793964992662	21.655331639044995	24.795074632208184
12	20.2833160325017	32.49991051293983	29.497261695958766	17.719511758599708
13	28.185023445609765	21.643877295343096	30.944625407166125	19.226473851881018
14	22.127644342628056	23.773311379174572	32.16666070086266	21.93238357733472
15	26.081182660987224	26.148476930235887	30.095751154383077	17.674589254393815
16	20.398575366002074	26.59734402405412	33.113075849232196	19.8910047607116
17	18.266277696245123	30.036331746429468	31.92898306904822	19.768407488277195
18	16.86007803271647	25.841357339728678	36.0679385760819	21.230626051472957
19	18.691520206178186	27.265812363532234	37.80237677631814	16.24029065397144
20	19.10691913949243	24.94845545334145	38.89161327272077	17.053012134445357
21	20.51365572538211	26.210401975874287	36.40476787056592	16.871174428177685
22	19.962236460607798	26.670007516913053	36.075455489136274	17.29230053334288
23	19.09725453699395	27.346708665926904	36.46293445967713	17.093102337402012
24	17.716469198553888	29.28768300103805	35.40304971901063	17.59279808139743
25	17.72702867165408	28.195403944589614	36.73748076028207	17.340086623474246
26	17.923542255789812	28.94727422414719	36.307226974979415	16.82195654508358
27	17.3055446182482	29.354082399685005	35.53030031857393	17.81007266349286
28	16.7194043741275	28.893403013924186	37.89454844829437	16.492644163653935
29	17.736156351791532	29.56885134409564	36.80602784837313	15.888964455739702
30	16.40440992232523	30.096467050864444	36.85399291262483	16.645130114185488
31	16.644772165944804	30.858180907040843	35.96610230160719	16.530944625407166
32	15.509002398253212	30.81826967820453	36.52647027239861	17.146257651143646
33	15.619071482263664	31.132906181766117	36.43948884991231	16.808533486057918
34	16.7240577012564	30.509718294734583	35.84457887389483	16.921645130114186
35	15.963417689802053	31.43304578157998	35.11042703225114	17.493109496366827
36	16.50374055911515	31.6528260013602	33.6580520456742	18.18538139385045
37	15.78140100941404	30.77549486344275	34.85753660020761	18.585567526935606
38	16.152414360883416	30.362601567813297	34.940222643805704	18.544761427497583
39	15.891112145183806	31.083151376310987	34.794895658087846	18.230840820417367
40	15.725024161506246	31.08476214339407	34.80241257114221	18.387801123957477
41	15.703905215305866	30.81003686866879	34.02262232881125	19.46343558721409
42	15.313204710598846	31.398861724594624	33.96946701506962	19.318466549736907
43	15.898987006478862	30.500411640476788	33.59380033647135	20.006801016573004
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1289.0
1	880.5
2	472.0
3	1021.5
4	1571.0
5	1571.0
6	2481.5
7	3392.0
8	3663.0
9	3934.0
10	5663.5
11	7393.0
12	7393.0
13	13524.5
14	19656.0
15	25420.5
16	31185.0
17	27961.0
18	24737.0
19	24737.0
20	25936.0
21	27135.0
22	19904.0
23	12673.0
24	11370.5
25	10068.0
26	10068.0
27	10337.0
28	10606.0
29	11543.5
30	12481.0
31	14315.0
32	16149.0
33	16149.0
34	18930.0
35	21711.0
36	23492.5
37	25274.0
38	29285.0
39	33296.0
40	33296.0
41	35168.0
42	37040.0
43	39736.5
44	42433.0
45	41971.0
46	41509.0
47	41509.0
48	41665.5
49	41822.0
50	39176.5
51	36531.0
52	31181.0
53	25831.0
54	25831.0
55	23731.5
56	21632.0
57	17864.5
58	14097.0
59	12413.0
60	10729.0
61	10729.0
62	9531.0
63	8333.0
64	6840.5
65	5348.0
66	4368.0
67	3388.0
68	3388.0
69	2858.5
70	2329.0
71	1973.0
72	1617.0
73	1370.0
74	1123.0
75	1123.0
76	979.5
77	836.0
78	693.5
79	551.0
80	426.5
81	302.0
82	302.0
83	224.0
84	146.0
85	103.5
86	61.0
87	47.5
88	34.0
89	34.0
90	29.0
91	24.0
92	12.5
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	558740.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.72606245337806
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.66949488930074	30.810684647296004
2	8.042446425447862	6.068196722487864
3	2.803182249434226	3.1725908583106923
4	1.5120388507077138	2.281730884549328
5	1.0072258618239263	1.8999332883913496
6	0.6844009340953081	1.5491851429697927
7	0.5394760478874878	1.424661495229346
8	0.41190766681714036	1.2431723490694946
9	0.34566228819242556	1.1736429364912513
>10	2.5136562861669143	18.77700688592415
>50	0.27752836403388437	7.217308061699233
>100	0.17817225436249612	12.859603078220939
>500	0.010508819292157948	2.7101397238419938
>1k	0.002388368020944988	1.5117602329483666
>5k	9.553472083779951E-4	2.9103501921702373
>10k+	9.553472083779951E-4	4.390033500399947
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	12788	2.2887210509360347	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	11573	2.0712674947202636	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	8926	1.597522998174464	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7224	1.2929090453520422	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2042	0.36546515373876937	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1973	0.3531159394351577	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1880	0.3364713462433332	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	1279	0.2289078999176719	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1215	0.2174535562157712	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	996	0.1782582238608297	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	984	0.17611053441672334	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	943	0.16877259548269322	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	833	0.14908544224505135	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	779	0.13942083974657266	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	753	0.13476751261767547	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	723	0.12939828900740954	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	704	0.12599778072090775	No Hit
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT	693	0.12402906539714359	TruSeq Adapter, Index 12 (95% over 22bp)
GAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTG	657	0.11758599706482444	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	653	0.11687010058345562	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	650	0.11633317822242904	No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGT	643	0.11508035938003365	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	604	0.1081003686866879	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	596	0.10666857572395033	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	585	0.10469986040018613	No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	560	0.10022550739163118	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	5.369223610265956E-4	0.0
3	0.0	1.789741203421985E-4	0.0	8.948706017109926E-4	0.0
4	0.0	1.789741203421985E-4	0.0	0.0010738447220531911	0.0
5	0.0	1.789741203421985E-4	0.0	0.0012528188423953897	0.0
6	0.0	1.789741203421985E-4	0.0	0.0012528188423953897	0.0
7	0.0	1.789741203421985E-4	0.0	0.0016107670830797867	0.0
8	0.0	1.789741203421985E-4	0.0	0.0017897412034219853	0.0
9	0.0	1.789741203421985E-4	0.0	0.003400508286501772	0.0
10	0.0	1.789741203421985E-4	0.0	0.005369223610265955	0.0
11	0.0	1.789741203421985E-4	0.0	0.006979990693345742	0.0
12	0.0	1.789741203421985E-4	0.0	0.011454343701900705	0.0
13	0.0	1.789741203421985E-4	0.0	0.013781007266349286	0.0
14	0.0	1.789741203421985E-4	0.0	0.017718437913877654	0.0
15	0.0	1.789741203421985E-4	0.0	0.026130221569960983	0.0
16	0.0	1.789741203421985E-4	0.0	0.04635429716862942	0.0
17	0.0	1.789741203421985E-4	0.0	0.073916311701328	0.0
18	0.0	3.57948240684397E-4	0.0	0.08250706947775352	0.0
19	0.0	5.369223610265956E-4	0.0	0.09915166266957798	0.0
20	0.0	5.369223610265956E-4	0.0	0.11293266993592727	0.0
21	0.0	5.369223610265956E-4	0.0	0.1449690374771808	0.0
22	0.0	5.369223610265956E-4	0.0	0.2076099795969503	0.0
23	0.0	5.369223610265956E-4	0.0	0.2895801267136772	0.0
24	0.0	5.369223610265956E-4	0.0	0.42488456169237926	0.0
25	0.0	5.369223610265956E-4	0.0	0.4596055410387658	0.0
26	0.0	5.369223610265956E-4	0.0	0.5132977771414253	0.0
27	0.0	5.369223610265956E-4	0.0	0.5578623331066328	0.0
28	0.0	5.369223610265956E-4	0.0	0.6156709739771629	0.0
29	0.0	5.369223610265956E-4	0.0	0.7051580341482622	0.0
30	0.0	5.369223610265956E-4	0.0	0.864623975373161	0.0
31	0.0	5.369223610265956E-4	0.0	1.3106274832659197	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCGC	20	0.0018411262	37.0	6
GCACACT	30	3.5971316E-4	30.833334	6
TAAGTTC	25	0.0054939566	29.599998	3
GGAACGC	40	5.9347483E-5	27.750002	8
GCCGTCT	125	0.0	25.16	36
GTCATGG	60	1.3353529E-6	24.666668	1
AAGGTCT	45	1.3220233E-4	24.666666	34
ACACTAC	45	1.3220233E-4	24.666666	8
CCGTCTT	140	0.0	23.785713	37
CAGCGTC	55	1.90039E-5	23.545454	2
AGCGTCA	55	1.90039E-5	23.545454	3
GCAGCGT	55	1.90039E-5	23.545454	1
CTGACTT	40	0.0019299515	23.125002	31
AGGTGTC	40	0.0019299515	23.125002	14
TCGCGCG	40	0.0019299515	23.125002	9
TGGTAAT	40	0.0019299515	23.125002	34
TTGGTAA	205	0.0	22.560976	33
GCCGAGT	75	3.7336576E-7	22.199999	12
GTAAACA	210	0.0	22.02381	36
TAAACAG	210	0.0	21.142857	37
>>END_MODULE