FastQCFastQC Report
Fri 10 Feb 2017
ERR1630624.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630624.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1442688
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT158731.1002378892733564No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT130510.9046308002839145No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT120190.8330976621417798No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75770.5252001818827078No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT42030.29133118179398454No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTC35700.2474547511312217No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC35330.24489009404666845No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG34300.2377506432437228No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA32740.2269374944547955No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCAT31440.21792653713068938No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTG30230.20953941531363676No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGA28420.19699339011622746No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC27870.1931810620175672No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC27840.19297311684854937No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAG27350.18957667908792475No Hit
CGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA26810.18583366604560378No Hit
GAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTG24970.17307969567917664No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGAC22950.15907805429864252No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG22820.15817695856623193No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC22650.1569986026084642No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG22520.1560975068760536No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT22210.15394874012953597No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA21270.14743312483364385No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21170.1467399742702511No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20350.1410561396504303No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC20200.14001641380534113No Hit
GTCCTAAACTACTAAACCTGCATTAAAAATTTCGGTTGGGGCG20030.13883805784757342No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC19980.138491482565877No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG19850.13759038683346642No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATG18890.13093614142489576No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGT18830.13052025108686008No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC18710.12968847041078876No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG18560.12864874456569958No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC18430.12774764883328898No Hit
GATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTT18250.12649997781918199No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC18130.12566819714311062No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC18040.12504436163605714No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC18020.12490573152337857No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC17710.12275696477686097No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG17420.12074682814302191No Hit
GCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGAT17350.12026162274864696No Hit
GAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTA17130.11873669150918285No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC16910.11721176026971873No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT16790.11637997959364742No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT16760.11617203442462957No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC16430.1138846375654334No Hit
GTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCG16170.11208244610061219No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGAT16170.11208244610061219No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT16110.11166655576257652No Hit
GGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACAT15880.11007230946677314No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT15650.10847806317096974No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT15510.10750765238221986No Hit
GGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAG15480.10729970721320202No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG15080.1045271049596309No Hit
ATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC14960.10369532428355958No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA14950.10362600922722029No Hit
GTCCTACGTGATCTGAGTTCAGACCGGAGTAATCCAGGTCGGT14810.10265559843847041No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC14760.10230902315677402No Hit
GTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTG14740.10217039304409546No Hit
ATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGAC14610.10126929731168485No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC14580.10106135214266701No Hit
GGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAAC14530.10071477686097063No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT14470.10029888652293495No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT14460.1002295714665957No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAAGGC507.3341653E-933.30000332
TCATGGA451.0802978E-732.888892
GACTAGA250.005496802329.616
TTCAATA358.870498E-426.4285729
GCCGTCT1900.025.3157936
CCGTCTT2050.023.46341537
CATTACA400.001931424823.12524
ATTCAAT400.001931424823.12528
AATACTG5100.022.4901965
AGTCATA502.702565E-422.210
AATCGTC603.727134E-521.58333428
GTAAACA5000.021.4636
TTGGTAA5100.021.4019633
ATACTGG5600.021.1428576
ATGCTAG5450.021.04587415
TCTGTCG450.003826275220.5555558
CGAATTT450.003826275220.55555518
AGCCTTA450.003826275220.5555556
CTCTAAT5850.020.5555551
TGCTAGA5500.020.5181816
Produced by FastQC (version 0.11.5)