##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630624.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1442688
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.884103839499602	33.0	31.0	34.0	30.0	34.0
2	32.038227253571115	33.0	31.0	34.0	30.0	34.0
3	32.102087214976486	33.0	31.0	34.0	30.0	34.0
4	35.71505828009937	37.0	35.0	37.0	33.0	37.0
5	35.718826246561974	37.0	35.0	37.0	35.0	37.0
6	35.79003499024044	37.0	35.0	37.0	35.0	37.0
7	35.77332520960873	37.0	35.0	37.0	35.0	37.0
8	35.77915737844912	37.0	35.0	37.0	35.0	37.0
9	37.4559121584154	39.0	37.0	39.0	35.0	39.0
10	37.350592782361815	39.0	37.0	39.0	34.0	39.0
11	37.483871772690975	39.0	37.0	39.0	35.0	39.0
12	37.42620580472008	39.0	37.0	39.0	34.0	39.0
13	37.48583754768876	39.0	37.0	39.0	35.0	39.0
14	38.658970615961316	40.0	38.0	41.0	34.0	41.0
15	38.609878227309025	40.0	38.0	41.0	34.0	41.0
16	38.55381967549463	40.0	38.0	41.0	34.0	41.0
17	38.61025668751664	40.0	38.0	41.0	34.0	41.0
18	38.610332240928045	40.0	38.0	41.0	34.0	41.0
19	38.66808554586993	40.0	38.0	41.0	34.0	41.0
20	38.636711471919085	40.0	38.0	41.0	34.0	41.0
21	38.55659089144708	40.0	38.0	41.0	34.0	41.0
22	38.49569137609795	40.0	38.0	41.0	34.0	41.0
23	38.44982560331825	40.0	38.0	41.0	34.0	41.0
24	38.40095086394286	40.0	38.0	41.0	34.0	41.0
25	38.315682254236535	40.0	38.0	41.0	34.0	41.0
26	38.082639489619375	40.0	37.0	41.0	33.0	41.0
27	37.930033382131136	40.0	37.0	41.0	33.0	41.0
28	37.803576379646884	40.0	37.0	41.0	33.0	41.0
29	37.69225570601544	40.0	37.0	41.0	33.0	41.0
30	37.53784186185786	40.0	36.0	41.0	32.0	41.0
31	37.448623680241326	39.0	36.0	41.0	32.0	41.0
32	37.27919203597729	39.0	36.0	41.0	32.0	41.0
33	37.18238454884216	39.0	35.0	41.0	32.0	41.0
34	37.05866271848106	39.0	35.0	41.0	31.0	41.0
35	36.90725021626297	39.0	35.0	41.0	31.0	41.0
36	36.815986547333864	39.0	35.0	41.0	31.0	41.0
37	36.55485246983409	39.0	35.0	41.0	30.0	41.0
38	36.37519824106113	39.0	35.0	41.0	30.0	41.0
39	36.2202097750865	39.0	35.0	41.0	30.0	41.0
40	36.02182939069293	39.0	35.0	41.0	29.0	41.0
41	35.8189851166711	39.0	35.0	41.0	28.0	41.0
42	35.58782217638186	39.0	35.0	40.0	26.0	41.0
43	34.71917628759649	38.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	6.0
10	13.0
11	12.0
12	3.0
13	4.0
14	1.0
15	5.0
16	11.0
17	28.0
18	59.0
19	127.0
20	337.0
21	619.0
22	1099.0
23	1783.0
24	3091.0
25	4763.0
26	7124.0
27	10589.0
28	14785.0
29	20146.0
30	26367.0
31	33307.0
32	42086.0
33	53611.0
34	69524.0
35	89718.0
36	125163.0
37	199443.0
38	319717.0
39	419145.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.089851721231476	20.460764905509716	12.02595432969568	23.423429043563125
2	20.609861591695502	20.772821289149142	33.580718769408215	25.03659834974714
3	21.473319248513885	25.444864031585485	28.704889761334396	24.37692695856623
4	13.982510424984474	16.13619854050217	34.355175893886965	35.52611514062639
5	13.472559555496408	38.77976388519209	33.0332684544406	14.714408104870907
6	32.1657212092982	34.55584298198918	16.249043452222516	17.02939235649011
7	28.044040014195726	31.738463202022892	21.692840031940378	18.524656751841007
8	28.691165380179225	30.645226133439802	19.399343447786354	21.264265038594623
9	28.415915291455953	12.538123280986602	21.63198141247449	37.413980015082956
10	20.46984517789016	26.749789282228733	30.55130423210008	22.22906130778103
11	33.802388319581226	22.952225290568716	20.178444681039835	23.06694170881022
12	23.57668463312927	28.638139362079674	25.504197719811906	22.28097828497915
13	29.31243623014817	20.059569359417974	26.99738266347263	23.63061174696123
14	23.090578143021915	22.215475556738532	26.346167709165115	28.34777859107444
15	27.169561263419396	26.595632596930173	25.0245375299441	21.210268609706326
16	24.93463590187206	25.147225179664627	26.288705527459854	23.62943339100346
17	24.3874628471298	26.260771559755124	25.6720094712093	23.679756121905776
18	22.130495297666577	23.55859340342472	29.462780587348064	24.848130711560643
19	23.252775374855826	24.786093736136987	29.68403424718304	22.27709664182415
20	23.54784956969213	23.09646992281075	31.14422522402626	22.211455283470855
21	23.50251752284624	25.232552014018278	29.5549696122793	21.70996085085618
22	25.371320756809514	25.122756964776862	27.38124944547955	22.124672832934078
23	22.989655420991927	24.244119310620178	29.49646770472895	23.269757563658946
24	22.45267167953154	26.54822109839411	27.406688071156065	23.592419150918285
25	22.298792254458345	25.16968325791855	29.526481124123862	23.005043363499244
26	22.756826146748292	26.83123447342738	28.377098859905953	22.034840519918376
27	23.421418906929286	26.038062283737023	28.141150408127054	22.399368401206637
28	22.025205727087215	25.773417398633665	29.708155886789108	22.493220987490016
29	22.372474159346996	25.91558257918552	30.08952732676781	21.62241593469967
30	19.96682581403602	26.851751730103807	30.983137033093783	22.198285422766393
31	23.09771759382486	27.0942157971786	27.798110194303966	22.009956414692574
32	20.616307891935055	26.143629114541746	28.705167021559756	24.534895971963447
33	20.261761378759648	26.443971253659836	29.556286598349747	23.737980769230766
34	22.551029744476978	25.49151306450182	30.12910622393754	21.828350967083665
35	21.079886988732145	27.609711871173808	29.5129646881377	21.797436451956347
36	21.81545836660456	27.183701534912608	28.131169040014196	22.869671058468636
37	21.351671324638453	25.557362368024133	29.564881665335818	23.526084642001596
38	21.4392855558513	24.905523578209564	29.207354604737823	24.447836261201314
39	21.39520118001952	26.393094002306803	29.464166888474846	22.74753792919883
40	20.46700326058025	26.813697764173543	29.102550239552833	23.61674873569337
41	21.208605048354183	25.70347850678733	29.348965264838967	23.73895118001952
42	20.05624223671369	27.525563392777926	29.99373391890693	22.424460451601455
43	20.33475013308491	24.673387454529323	29.55677180374412	25.435090608641648
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	176.0
1	223.0
2	270.0
3	1018.0
4	1766.0
5	1766.0
6	2726.5
7	3687.0
8	3734.5
9	3782.0
10	5409.5
11	7037.0
12	7037.0
13	12809.5
14	18582.0
15	25129.5
16	31677.0
17	28646.0
18	25615.0
19	25615.0
20	27423.0
21	29231.0
22	20715.5
23	12200.0
24	11441.5
25	10683.0
26	10683.0
27	11975.0
28	13267.0
29	16574.0
30	19881.0
31	25343.5
32	30806.0
33	30806.0
34	40606.5
35	50407.0
36	56176.5
37	61946.0
38	73344.0
39	84742.0
40	84742.0
41	89612.5
42	94483.0
43	101421.0
44	108359.0
45	103907.0
46	99455.0
47	99455.0
48	104191.0
49	108927.0
50	108831.5
51	108736.0
52	103647.0
53	98558.0
54	98558.0
55	93276.5
56	87995.0
57	80322.5
58	72650.0
59	69876.5
60	67103.0
61	67103.0
62	60842.5
63	54582.0
64	50611.0
65	46640.0
66	42390.5
67	38141.0
68	38141.0
69	28582.5
70	19024.0
71	16738.0
72	14452.0
73	10475.0
74	6498.0
75	6498.0
76	5133.5
77	3769.0
78	3027.5
79	2286.0
80	1964.5
81	1643.0
82	1643.0
83	1529.0
84	1415.0
85	1246.0
86	1077.0
87	871.5
88	666.0
89	666.0
90	522.5
91	379.0
92	225.5
93	72.0
94	42.5
95	13.0
96	13.0
97	11.0
98	9.0
99	5.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1442688.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.419495522882507
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.5121824258671	12.80545132644714
2	10.380114924772856	3.6163273091817336
3	3.78716606475938	1.9791156692846577
4	2.1210821575482957	1.4779272458831403
5	1.384239640569693	1.205637811075013
6	0.9950747956304191	1.0400220568450387
7	0.7676996066512142	0.9361057902685641
8	0.6246816438661601	0.8705311278842769
9	0.49768508708530423	0.7802480831659062
>10	4.442455256772665	16.272903357080455
>50	0.7009510496261042	8.623201750591413
>100	0.6480793112039857	22.44634969481828
>500	0.08731847332661857	10.551508438729291
>1k	0.04966738849770965	14.009301393734324
>5k	4.0054345562669073E-4	0.528667363898324
>10k+	0.0012016303668800723	2.8567015811124556
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	15873	1.1002378892733564	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	13051	0.9046308002839145	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	12019	0.8330976621417798	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7577	0.5252001818827078	No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT	4203	0.29133118179398454	No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTC	3570	0.2474547511312217	No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC	3533	0.24489009404666845	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	3430	0.2377506432437228	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	3274	0.2269374944547955	No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCAT	3144	0.21792653713068938	No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTG	3023	0.20953941531363676	No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGA	2842	0.19699339011622746	No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC	2787	0.1931810620175672	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	2784	0.19297311684854937	No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAG	2735	0.18957667908792475	No Hit
CGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA	2681	0.18583366604560378	No Hit
GAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTG	2497	0.17307969567917664	No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGAC	2295	0.15907805429864252	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	2282	0.15817695856623193	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	2265	0.1569986026084642	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	2252	0.1560975068760536	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2221	0.15394874012953597	No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA	2127	0.14743312483364385	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2117	0.1467399742702511	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2035	0.1410561396504303	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	2020	0.14001641380534113	No Hit
GTCCTAAACTACTAAACCTGCATTAAAAATTTCGGTTGGGGCG	2003	0.13883805784757342	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	1998	0.138491482565877	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	1985	0.13759038683346642	No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATG	1889	0.13093614142489576	No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGT	1883	0.13052025108686008	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	1871	0.12968847041078876	No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG	1856	0.12864874456569958	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1843	0.12774764883328898	No Hit
GATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTT	1825	0.12649997781918199	No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC	1813	0.12566819714311062	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	1804	0.12504436163605714	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	1802	0.12490573152337857	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	1771	0.12275696477686097	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	1742	0.12074682814302191	No Hit
GCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGAT	1735	0.12026162274864696	No Hit
GAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTA	1713	0.11873669150918285	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	1691	0.11721176026971873	No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT	1679	0.11637997959364742	No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT	1676	0.11617203442462957	No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC	1643	0.1138846375654334	No Hit
GTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCG	1617	0.11208244610061219	No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGAT	1617	0.11208244610061219	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	1611	0.11166655576257652	No Hit
GGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACAT	1588	0.11007230946677314	No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT	1565	0.10847806317096974	No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT	1551	0.10750765238221986	No Hit
GGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAG	1548	0.10729970721320202	No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG	1508	0.1045271049596309	No Hit
ATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC	1496	0.10369532428355958	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	1495	0.10362600922722029	No Hit
GTCCTACGTGATCTGAGTTCAGACCGGAGTAATCCAGGTCGGT	1481	0.10265559843847041	No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC	1476	0.10230902315677402	No Hit
GTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTG	1474	0.10217039304409546	No Hit
ATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGAC	1461	0.10126929731168485	No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC	1458	0.10106135214266701	No Hit
GGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAAC	1453	0.10071477686097063	No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT	1447	0.10029888652293495	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	1446	0.1002295714665957	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.0794516901783338E-4	0.0
2	0.0	0.0	0.0	0.0018715065211605004	0.0
3	0.0	0.0	0.0	0.0029112323662496675	0.0
4	0.0	0.0	0.0	0.004505478662053057	0.0
5	0.0	0.0	0.0	0.0051986292254458345	0.0
6	0.0	0.0	0.0	0.005545204507142224	0.0
7	0.0	0.0	0.0	0.0064463002395528345	0.0
8	0.0	0.0	0.0	0.006792875521249224	0.0
9	0.0	0.0	0.0	0.010535888563570224	0.0
10	0.0	0.0	0.0	0.01504136722562328	0.0
11	0.0	0.0	0.0	0.016427668352408838	0.0
12	0.0	0.0	0.0	0.01816054476089078	0.0
13	0.0	0.0	0.0	0.019616160944015616	0.0
14	0.0	0.0	0.0	0.021487667465176115	0.0
15	0.0	0.0	0.0	0.02453752994410434	0.0
16	0.0	0.0	0.0	0.02925095377517523	0.0
17	0.0	0.0	0.0	0.035073418507674564	0.0
18	0.0	0.0	0.0	0.03937095200070979	0.0
19	0.0	0.0	0.0	0.044777526395173455	0.0
20	0.0	0.0	0.0	0.04886711471919084	0.0
21	0.0	0.0	0.0	0.0571156064235649	0.0
22	0.0	0.0	0.0	0.06924574128293852	0.0
23	0.0	0.0	0.0	0.08858464200159702	0.0
24	0.0	0.0	0.0	0.12150929376275398	0.0
25	0.0	0.0	0.0	0.13176792210096708	0.0
26	0.0	0.0	0.0	0.15082956259426847	0.0
27	0.0	0.0	0.0	0.1732183257918552	0.0
28	0.0	0.0	0.0	0.21154955194747582	0.0
29	0.0	0.0	0.0	0.2663084464555053	0.0
30	0.0	0.0	0.0	0.36307226510513707	0.0
31	0.0	0.0	0.0	0.6564828985893	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAAGGC	50	7.3341653E-9	33.300003	32
TCATGGA	45	1.0802978E-7	32.88889	2
GACTAGA	25	0.0054968023	29.6	16
TTCAATA	35	8.870498E-4	26.42857	29
GCCGTCT	190	0.0	25.31579	36
CCGTCTT	205	0.0	23.463415	37
CATTACA	40	0.0019314248	23.125	24
ATTCAAT	40	0.0019314248	23.125	28
AATACTG	510	0.0	22.490196	5
AGTCATA	50	2.702565E-4	22.2	10
AATCGTC	60	3.727134E-5	21.583334	28
GTAAACA	500	0.0	21.46	36
TTGGTAA	510	0.0	21.40196	33
ATACTGG	560	0.0	21.142857	6
ATGCTAG	545	0.0	21.045874	15
TCTGTCG	45	0.0038262752	20.555555	8
CGAATTT	45	0.0038262752	20.555555	18
AGCCTTA	45	0.0038262752	20.555555	6
CTCTAAT	585	0.0	20.555555	1
TGCTAGA	550	0.0	20.51818	16
>>END_MODULE