FastQCFastQC Report
Fri 10 Feb 2017
ERR1630623.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630623.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1427504
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT86550.6063030296237348No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT70390.49309844315672674No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT63320.44357143657741066No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51350.3597187818738161No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15310.1072501373025925No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG1750.021.14285929
TCTAGCG1950.019.92307728
AAATGCG759.268348E-619.73333434
TGCGTTA801.6171169E-518.537
CGGTCCA2200.017.6590910
ACACCGT752.0677078E-417.2666666
GGTATCA46450.017.1259421
CTTGCGC2350.016.5319143
TTAACGG1352.2204404E-816.44444535
GTCTTGC4550.016.2637351
CCGGTCC2300.016.0869569
TAACGGC1403.4746336E-815.85714236
CGTTTAG1059.3461895E-615.85714226
TACCGTC1659.749783E-1015.6969717
TATTAGA2950.015.6779662
AATGCGT957.061591E-515.57894635
ATGCGTT957.061591E-515.57894636
TCTTGCG2500.015.542
GCAATAC2400.015.41666637
GTTTAAC2055.456968E-1215.34146333
Produced by FastQC (version 0.11.5)