FastQCFastQC Report
Fri 10 Feb 2017
ERR1630622.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630622.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1240009
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT52920.42677109601623864No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT46360.3738682541820261No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT34420.277578630477682No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24040.19386956062415675No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC21690.17491808527196173No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT19360.1561278990717003No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA18270.14733764029132046No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA15510.12507973732448716No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG13790.11120887025819974No Hit
GGGTACACTGGCTCCAGTGGGGCTCCCTGGGCACCCAGCAGTG12640.1019347440220192No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACTCG358.869792E-426.4285719
CACCGTA603.7265905E-521.58333431
CGTATTA450.00382597620.55555515
AGTCGGT1850.019.011
AAGACGG2850.018.8245625
CGTAGTA500.007035187418.52
CGCAAGA2800.018.52
TACGTGT701.2192958E-418.57
GTCGGTG2050.018.0487812
GCTTTAT5450.017.9908261
GACGGAC2900.017.8620687
TCGGTGA2250.017.26666613
CGGTGAT2150.017.20930314
CGAACGA1401.869921E-917.17857216
GCAGTCG2100.016.7380949
CAGTCGG2100.016.73809410
GCGCAAG3000.016.651
CCTATAC904.446412E-516.4444453
ACGGACC3050.016.377058
TCTAGCG2150.016.34883728
Produced by FastQC (version 0.11.5)