##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630622.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1240009
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.696238495043183	31.0	31.0	34.0	30.0	34.0
2	31.864518725267317	33.0	31.0	34.0	30.0	34.0
3	31.96462122452337	33.0	31.0	34.0	30.0	34.0
4	35.61690761921889	37.0	35.0	37.0	33.0	37.0
5	35.57057408454293	37.0	35.0	37.0	33.0	37.0
6	35.60500770558923	37.0	35.0	37.0	33.0	37.0
7	35.576815168277	37.0	35.0	37.0	33.0	37.0
8	35.57490227893507	37.0	35.0	37.0	33.0	37.0
9	37.24150550520198	39.0	37.0	39.0	34.0	39.0
10	37.109031466707094	39.0	37.0	39.0	33.0	39.0
11	37.23173864060664	39.0	37.0	39.0	34.0	39.0
12	37.1496214946827	39.0	37.0	39.0	33.0	39.0
13	37.21969276029448	39.0	37.0	39.0	34.0	39.0
14	38.39817533582418	40.0	38.0	41.0	34.0	41.0
15	38.36486186793805	40.0	38.0	41.0	34.0	41.0
16	38.33874270267393	40.0	38.0	41.0	34.0	41.0
17	38.3229452366878	40.0	38.0	41.0	34.0	41.0
18	38.31204934802893	40.0	38.0	41.0	34.0	41.0
19	38.31928558583043	40.0	38.0	41.0	34.0	41.0
20	38.300199434036365	40.0	38.0	41.0	34.0	41.0
21	38.21871212225073	40.0	38.0	41.0	34.0	41.0
22	38.15142067517252	40.0	38.0	41.0	33.0	41.0
23	38.1311159838356	40.0	38.0	41.0	33.0	41.0
24	38.07216076657508	40.0	37.0	41.0	33.0	41.0
25	38.0102975059052	40.0	37.0	41.0	33.0	41.0
26	37.785870102555705	40.0	37.0	41.0	33.0	41.0
27	37.65151381965776	40.0	37.0	41.0	32.0	41.0
28	37.534522733302744	40.0	37.0	41.0	32.0	41.0
29	37.42387918152207	39.0	37.0	41.0	32.0	41.0
30	37.33743303476023	39.0	36.0	41.0	31.0	41.0
31	37.280489093224325	39.0	36.0	41.0	31.0	41.0
32	37.186439775840334	39.0	36.0	41.0	31.0	41.0
33	37.125619249537706	39.0	36.0	41.0	31.0	41.0
34	37.04560450770922	39.0	36.0	41.0	31.0	41.0
35	36.9730526149407	39.0	36.0	41.0	31.0	41.0
36	36.923323137170776	39.0	36.0	40.0	31.0	41.0
37	36.71675044294033	39.0	35.0	40.0	30.0	41.0
38	36.5843514039011	39.0	35.0	40.0	30.0	41.0
39	36.50706567452333	39.0	35.0	40.0	30.0	41.0
40	36.377140004629	39.0	35.0	40.0	30.0	41.0
41	36.23944422984027	39.0	35.0	40.0	30.0	41.0
42	36.140924783610444	39.0	35.0	40.0	29.0	41.0
43	35.22736367236044	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	4.0
11	2.0
12	2.0
13	0.0
14	3.0
15	4.0
16	14.0
17	35.0
18	66.0
19	131.0
20	272.0
21	484.0
22	960.0
23	1669.0
24	2670.0
25	4286.0
26	6291.0
27	9118.0
28	12510.0
29	17402.0
30	23019.0
31	29565.0
32	37646.0
33	47886.0
34	62238.0
35	82659.0
36	115296.0
37	177714.0
38	315423.0
39	292637.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.23556603218202	19.201150959388197	11.924913448208843	24.638369560220934
2	19.9825162559304	20.610656858135705	32.78266528710679	26.624161598827108
3	21.65653636384897	22.635238937781903	28.561970114733036	27.146254583636086
4	15.198438075852675	14.874892037073925	33.38056417332455	36.54610571374885
5	15.057310067910798	36.58328286326954	33.64612676198318	14.713280306836483
6	36.698120739446246	33.43411217176649	14.720699607825427	15.147067480961832
7	29.928169876186384	29.644704191663124	20.733962414788927	19.69316351736157
8	28.762371886010506	31.34146607000433	19.384214146832804	20.51194789715236
9	26.679322488788387	13.2315975125987	19.86808160263353	40.220998395979386
10	18.00374029543334	26.270454488636773	31.983961406731726	23.74184380919816
11	36.4401387409285	21.3194420363078	20.71275289130966	21.527666331454046
12	23.180396271317385	25.265623072090605	27.380123853939768	24.17385680265224
13	31.095742047033532	19.109135498210094	24.73635272002058	25.058769734735797
14	23.48950693099808	20.735010794276494	24.70699809436867	31.068484180356755
15	26.728999547583932	27.46165552024219	21.851857526840533	23.95748740533335
16	26.01400473706239	25.61618504381823	23.787407994619393	24.582402224499983
17	24.560708833564917	25.783199960645447	24.751755834030238	24.904335371759398
18	23.98353560337062	24.07393817302939	26.947223770150053	24.995302453449934
19	25.194655845239833	24.51095113019341	26.718596397284212	23.575796627282543
20	25.754329202449338	23.85982682383757	25.851263982761413	24.53457999095168
21	25.806103020220018	24.208856548621824	26.022149839235038	23.962890591923124
22	25.657797645017094	24.41740342207194	26.05497218165352	23.86982675125745
23	24.717320600092417	24.212324265388396	25.924005390283455	25.146349744235728
24	24.404177711613386	25.139817533582416	26.215777466131296	24.240227288672905
25	24.89320642027598	24.84401322893624	25.594249719155265	24.668530631632514
26	24.765626701096526	25.332638714719007	25.853602675464455	24.048131908720016
27	25.011431368643294	24.158695622370484	25.914811908623243	24.91506110036298
28	24.169824573853898	24.862642125984568	26.218761315442062	24.74877198471947
29	24.298130094217058	25.354574039381973	26.441098411382498	23.90619745501847
30	24.45667733056776	25.1947364898158	25.86626387389124	24.4823223057252
31	23.930471472384475	25.882634722812497	26.062149548914565	24.124744255888466
32	23.693698997345987	25.00893138678832	25.757393696336074	25.539975919529617
33	23.1689447415301	25.456105560524158	26.490614181026107	24.884335516919634
34	25.140059467310316	24.362161887534686	25.979408213972636	24.518370431182355
35	24.275307679218457	24.64498241545021	26.316663830665743	24.76304607466559
36	23.35095954948714	25.582233677336212	26.487549687139367	24.57925708603728
37	24.2822431127516	24.254340089467092	26.94561087863072	24.517805919150586
38	24.09772832293959	24.36691991751673	26.777225004012067	24.758126755531613
39	23.94918101400877	24.089583220766947	27.021416780039498	24.939818985184786
40	23.27216979876759	24.096357365148155	27.52568731355982	25.10578552252443
41	22.25556427413027	24.471354643393717	28.162860108273406	25.11022097420261
42	23.042332757262248	24.257969095385597	28.376326300857496	24.323371846494663
43	23.074026075617194	23.915149002950788	28.060522141371553	24.95030278006047
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	142.0
1	164.5
2	187.0
3	499.5
4	812.0
5	812.0
6	1085.5
7	1359.0
8	1420.5
9	1482.0
10	2043.0
11	2604.0
12	2604.0
13	4435.0
14	6266.0
15	8852.5
16	11439.0
17	10844.5
18	10250.0
19	10250.0
20	12553.5
21	14857.0
22	13580.0
23	12303.0
24	13757.0
25	15211.0
26	15211.0
27	17285.5
28	19360.0
29	22201.0
30	25042.0
31	28588.0
32	32134.0
33	32134.0
34	36242.0
35	40350.0
36	44615.0
37	48880.0
38	53896.5
39	58913.0
40	58913.0
41	61306.0
42	63699.0
43	65414.0
44	67129.0
45	69199.5
46	71270.0
47	71270.0
48	76694.0
49	82118.0
50	82858.0
51	83598.0
52	83674.0
53	83750.0
54	83750.0
55	81657.5
56	79565.0
57	78285.0
58	77005.0
59	78087.5
60	79170.0
61	79170.0
62	73606.5
63	68043.0
64	62725.0
65	57407.0
66	50138.5
67	42870.0
68	42870.0
69	38357.5
70	33845.0
71	28494.0
72	23143.0
73	17228.0
74	11313.0
75	11313.0
76	8357.5
77	5402.0
78	4350.5
79	3299.0
80	2805.5
81	2312.0
82	2312.0
83	1786.0
84	1260.0
85	1136.5
86	1013.0
87	869.0
88	725.0
89	725.0
90	572.5
91	420.0
92	233.5
93	47.0
94	30.0
95	13.0
96	13.0
97	7.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1240009.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.76291238807965
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.92462191999672	50.787822327197986
2	7.622283145699139	8.80570547084311
3	1.7425657663859406	3.0196702108265385
4	0.6967677030767357	1.6098932715065992
5	0.3804120680859324	1.098685448010795
6	0.24633428519198985	0.8537391440235083
7	0.1766317943161882	0.7141936802024698
8	0.12642213520485027	0.584200857580138
9	0.10122028305881677	0.5262100508000751
>10	0.7496051096083147	8.718070077346095
>50	0.10958447287489756	4.383857778886053
>100	0.11261261076916675	12.976450034441092
>500	0.008694868658274473	3.2798763353735048
>1k	0.0021035972560341466	2.212938371649915
>5k	1.402398170689431E-4	0.428686941312371
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5292	0.42677109601623864	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4636	0.3738682541820261	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3442	0.277578630477682	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2404	0.19386956062415675	No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC	2169	0.17491808527196173	No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT	1936	0.1561278990717003	No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA	1827	0.14733764029132046	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	1551	0.12507973732448716	No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG	1379	0.11120887025819974	No Hit
GGGTACACTGGCTCCAGTGGGGCTCCCTGGGCACCCAGCAGTG	1264	0.1019347440220192	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.41933727900362E-4	0.0
3	0.0	0.0	0.0	4.032228798339367E-4	0.0
4	0.0	0.0	0.0	9.67734911601448E-4	0.0
5	0.0	1.6128915193357467E-4	0.0	0.0012096686395018101	0.0
6	0.0	1.6128915193357467E-4	0.0	0.0012096686395018101	0.0
7	0.0	2.41933727900362E-4	0.0	0.001451602367402172	0.0
8	0.0	2.41933727900362E-4	0.0	0.0015322469433689595	0.0
9	0.0	2.41933727900362E-4	0.0	0.0024193372790036203	0.0
10	0.0	2.41933727900362E-4	0.0	0.00395158422237258	0.0
11	0.0	2.41933727900362E-4	0.0	0.004193517950272942	0.0
12	0.0	2.41933727900362E-4	0.0	0.004274162526239729	0.0
13	0.0	2.41933727900362E-4	0.0	0.004516096254140091	0.0
14	0.0	2.41933727900362E-4	0.0	0.0054031865897747515	0.0
15	0.0	2.41933727900362E-4	0.0	0.0065322106533097744	0.0
16	0.0	2.41933727900362E-4	0.0	0.008628969628446245	0.0
17	8.064457596678733E-5	3.2257830386714934E-4	0.0	0.011128951483416652	0.0
18	8.064457596678733E-5	4.83867455800724E-4	0.0	0.012338620122918463	0.0
19	8.064457596678733E-5	4.83867455800724E-4	0.0	0.014757957401922083	0.0
20	8.064457596678733E-5	4.83867455800724E-4	0.0	0.016451493497224617	0.0
21	8.064457596678733E-5	4.83867455800724E-4	0.0	0.01967727653589611	0.0
22	8.064457596678733E-5	4.83867455800724E-4	0.0	0.024677240245836927	0.0
23	8.064457596678733E-5	4.83867455800724E-4	0.0	0.033225565298316384	0.0
24	8.064457596678733E-5	5.645120317675113E-4	0.0	0.046773854060736655	0.0
25	8.064457596678733E-5	7.25801183701086E-4	0.0	0.051612528618743894	0.0
26	8.064457596678733E-5	7.25801183701086E-4	0.0	0.058789895879787966	0.0
27	8.064457596678733E-5	7.25801183701086E-4	0.0	0.07217689549027467	0.0
28	8.064457596678733E-5	7.25801183701086E-4	0.0	0.10717664145986038	0.0
29	8.064457596678733E-5	7.25801183701086E-4	0.0	0.15588596534379992	0.0
30	8.064457596678733E-5	7.25801183701086E-4	0.0	0.2229016079722002	0.0
31	8.064457596678733E-5	7.25801183701086E-4	0.0	0.4562870108200828	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTACTCG	35	8.869792E-4	26.42857	19
CACCGTA	60	3.7265905E-5	21.583334	31
CGTATTA	45	0.003825976	20.555555	15
AGTCGGT	185	0.0	19.0	11
AAGACGG	285	0.0	18.824562	5
CGTAGTA	50	0.0070351874	18.5	2
CGCAAGA	280	0.0	18.5	2
TACGTGT	70	1.2192958E-4	18.5	7
GTCGGTG	205	0.0	18.04878	12
GCTTTAT	545	0.0	17.990826	1
GACGGAC	290	0.0	17.862068	7
TCGGTGA	225	0.0	17.266666	13
CGGTGAT	215	0.0	17.209303	14
CGAACGA	140	1.869921E-9	17.178572	16
GCAGTCG	210	0.0	16.738094	9
CAGTCGG	210	0.0	16.738094	10
GCGCAAG	300	0.0	16.65	1
CCTATAC	90	4.446412E-5	16.444445	3
ACGGACC	305	0.0	16.37705	8
TCTAGCG	215	0.0	16.348837	28
>>END_MODULE