FastQCFastQC Report
Fri 10 Feb 2017
ERR1630621.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630621.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2265447
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT102750.45355287499553065No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT89270.3940502691080392No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT77690.34293452903555016No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64350.28404990273442726No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTAGA555.145214E-420.1818182
CGGTCTA500.007036938318.520
AATTCGC752.0683826E-417.26666629
GGTATCA50600.017.1106721
CGAACTA1606.311893E-1016.187524
ATTCGCG700.002593535515.85714330
GACGGAC3700.015.57
CGAATTA855.3670077E-415.23529315
TCGTCGA750.00410604114.89
CAAGACG4800.014.6458344
CGGACCA3950.014.5189879
CCGGTCT2450.014.346949
TTAACGG1551.2116652E-714.32258135
GCGAACT1956.730261E-1014.2307723
CGTCAGA4600.014.0760871
CTTATAC21250.013.92941137
CGCGCTT1203.30314E-513.87500112
AAGACGG4150.013.8192775
TACTGGT3800.013.6315797
CGTTATT2850.013.6315782
Produced by FastQC (version 0.11.5)