FastQCFastQC Report
Fri 10 Feb 2017
ERR1630620.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630620.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2134973
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT84900.397663108620109No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT68020.31859887689446187No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT59720.2797225070293629No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40550.18993214434093547No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAATA2800.017.83928536
GGTATCA39850.017.455461
GACGGAC2950.016.3050847
ACGGGAC1403.477362E-815.8571423
ATTGCCG4750.015.57894711
AGTCCGT855.366826E-415.2352946
TTTGCGC2800.015.19642817
TTGCGCG2850.014.92982618
AGTCGGT3100.014.91935411
CAAGACG3500.014.84
GGTCTAG1508.110874E-814.81
ACGGACC3400.014.6911768
CGGTCCA3800.014.11842210
CGGACCA3700.014.09
TCTTATA20900.013.98564737
ATTTGCG3200.013.87500116
TAGACAG2800.013.8755
CCGGTCC3900.013.7564119
TCTTGCG3650.013.6849322
CGTAGAC950.001245685913.6315793
Produced by FastQC (version 0.11.5)