##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630620.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2134973
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.99339148551293	33.0	31.0	34.0	30.0	34.0
2	32.161747244578734	33.0	31.0	34.0	30.0	34.0
3	32.26163609563212	34.0	31.0	34.0	30.0	34.0
4	35.8476069720788	37.0	35.0	37.0	35.0	37.0
5	35.822786049284936	37.0	35.0	37.0	35.0	37.0
6	35.85479722694385	37.0	35.0	37.0	35.0	37.0
7	35.83116414118586	37.0	35.0	37.0	35.0	37.0
8	35.824529865248884	37.0	35.0	37.0	35.0	37.0
9	37.55004723713134	39.0	37.0	39.0	35.0	39.0
10	37.456636219755474	39.0	37.0	39.0	34.0	39.0
11	37.56790928971935	39.0	37.0	39.0	35.0	39.0
12	37.49789997344229	39.0	37.0	39.0	35.0	39.0
13	37.546733377892835	39.0	37.0	39.0	35.0	39.0
14	38.81696396160513	40.0	38.0	41.0	35.0	41.0
15	38.789994533888716	40.0	38.0	41.0	35.0	41.0
16	38.76790900868536	40.0	38.0	41.0	35.0	41.0
17	38.7580123964097	40.0	38.0	41.0	35.0	41.0
18	38.748649280342185	40.0	38.0	41.0	35.0	41.0
19	38.78185906800695	40.0	38.0	41.0	35.0	41.0
20	38.76080259563002	40.0	38.0	41.0	35.0	41.0
21	38.68857311076065	40.0	38.0	41.0	34.0	41.0
22	38.629295077736344	40.0	38.0	41.0	34.0	41.0
23	38.60562498916848	40.0	38.0	41.0	34.0	41.0
24	38.55320559089038	40.0	38.0	41.0	34.0	41.0
25	38.50672865652165	40.0	38.0	41.0	34.0	41.0
26	38.31981388055024	40.0	38.0	41.0	34.0	41.0
27	38.19706947113617	40.0	38.0	41.0	33.0	41.0
28	38.10914751615126	40.0	38.0	41.0	33.0	41.0
29	37.99303644589416	40.0	37.0	41.0	33.0	41.0
30	37.92099712736414	40.0	37.0	41.0	33.0	41.0
31	37.87447569594557	40.0	37.0	41.0	33.0	41.0
32	37.75839788137836	40.0	37.0	41.0	33.0	41.0
33	37.719710272682605	40.0	37.0	41.0	33.0	41.0
34	37.63449889061829	40.0	37.0	41.0	33.0	41.0
35	37.56993976036231	40.0	37.0	41.0	32.0	41.0
36	37.522960711915324	40.0	37.0	41.0	32.0	41.0
37	37.331093648491105	40.0	36.0	41.0	31.0	41.0
38	37.20772768554919	40.0	36.0	41.0	31.0	41.0
39	37.13706730717438	40.0	36.0	41.0	31.0	41.0
40	37.02836101440159	39.0	36.0	41.0	31.0	41.0
41	36.894007558877796	39.0	35.0	41.0	31.0	41.0
42	36.79623817256705	39.0	35.0	41.0	30.0	41.0
43	35.94583678575795	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	2.0
10	2.0
11	8.0
12	6.0
13	1.0
14	0.0
15	5.0
16	13.0
17	29.0
18	88.0
19	162.0
20	328.0
21	667.0
22	1161.0
23	2023.0
24	3395.0
25	5358.0
26	8294.0
27	11806.0
28	16842.0
29	23670.0
30	32315.0
31	41355.0
32	53201.0
33	68745.0
34	90899.0
35	120483.0
36	169377.0
37	260577.0
38	468118.0
39	756042.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.66664121747675	19.085534102773195	12.060480390150133	25.187344289599917
2	19.019537952002203	21.01525405707707	34.451255355454144	25.513952635466588
3	20.905463441458043	23.255984970301732	29.003973352356212	26.834578235884017
4	14.71845311392697	15.547409733050488	33.79977170671479	35.93436544630775
5	14.49526527970143	36.79971596830499	34.02722188992554	14.677796862068046
6	35.11594760214766	33.98534782407084	15.661228502655536	15.237476071125958
7	28.843924489911583	30.32520785977153	21.4191935916754	19.41167405864149
8	27.10437087494783	32.9391050847013	20.193697999927867	19.762826040422993
9	26.534621280924863	13.792914477138588	20.17917790997825	39.493286331958295
10	17.291085179999936	26.606050755677003	32.8238343061013	23.279029758221768
11	35.16241188998643	21.818964455288192	21.423643296659957	21.59498035806542
12	21.60359873403551	25.68294774688017	28.83099692595644	23.882456593127877
13	29.832180547482334	20.062923512381655	25.566833866283083	24.538062073852927
14	22.997855242197442	21.413479233695227	25.377042238941666	30.21162328516567
15	25.97147598587898	27.439035528786548	22.7047836202144	23.884704865120074
16	25.306783739185462	26.270870872840078	24.420730379260068	24.001615008714396
17	23.43790764567046	26.544644826890085	25.826977671380387	24.190469856059067
18	23.28287055620844	24.958582614393716	27.41065109488504	24.3478957345128
19	23.93177805995673	25.489127965552726	27.24540310345845	23.333690871032093
20	24.503775925971897	24.681483091355254	27.20540259759725	23.609338385075596
21	24.639093796502344	25.255401356363755	26.41663384033428	23.688871006799616
22	24.56204364176971	25.019145441183564	26.532373008932662	23.88643790811406
23	23.857866118213206	25.32491043212256	26.794249857023956	24.02297359264028
24	23.957398992867827	25.79264468449952	26.543145978895282	23.706810343737367
25	24.2580116938247	25.33118685810078	26.498976802048553	23.91182464602597
26	23.886203713114874	26.065341341553268	26.498133700051476	23.550321245280383
27	24.43623408820627	25.238070926423894	26.16042451122333	24.16527047414651
28	23.489290028492164	25.477090342594494	27.00797621328232	24.025643415631016
29	23.184321300550405	25.909086438095468	27.1360808778378	23.770511383516325
30	23.161370190630045	26.42843726829332	27.118890964897446	23.291301576179183
31	23.785687219463668	26.166092030203657	26.348857807569466	23.699362942763212
32	23.025958642099923	25.926088995036473	26.735139039229068	24.31281332363454
33	23.026989100096348	25.638919087032953	27.0953309479792	24.2387608648915
34	23.861472721200688	25.466411050631553	26.823243197923347	23.848873030244413
35	23.760113125552408	25.529081632414087	27.300111055268616	23.410694186764893
36	23.16038657163346	25.926042156036633	26.86230692378779	24.051264348542112
37	23.76503122053534	25.113479186856225	27.03711007118123	24.084379521427206
38	23.055888762996066	25.104813971886298	27.78241223659503	24.056885028522608
39	23.23888873536106	25.065281856023475	27.549247695404112	24.14658171321136
40	22.676633381312083	25.067670645015184	28.197967843153048	24.057728130519685
41	22.103558218300652	25.026592842157726	28.750433846235996	24.11941509330563
42	22.81771244882254	24.9108068345595	28.372349439547946	23.899131277070015
43	22.115876875257907	24.574315459727124	28.480453851172825	24.829353813842143
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	214.0
1	231.0
2	248.0
3	735.5
4	1223.0
5	1223.0
6	1794.5
7	2366.0
8	2368.5
9	2371.0
10	3458.0
11	4545.0
12	4545.0
13	7969.0
14	11393.0
15	15472.0
16	19551.0
17	19344.5
18	19138.0
19	19138.0
20	23046.5
21	26955.0
22	26195.5
23	25436.0
24	28186.0
25	30936.0
26	30936.0
27	35034.0
28	39132.0
29	45854.0
30	52576.0
31	59511.5
32	66447.0
33	66447.0
34	73026.5
35	79606.0
36	87157.0
37	94708.0
38	103508.5
39	112309.0
40	112309.0
41	116351.5
42	120394.0
43	121247.5
44	122101.0
45	125791.0
46	129481.0
47	129481.0
48	133828.0
49	138175.0
50	142159.0
51	146143.0
52	150659.0
53	155175.0
54	155175.0
55	148768.0
56	142361.0
57	136005.5
58	129650.0
59	120550.0
60	111450.0
61	111450.0
62	103977.5
63	96505.0
64	86160.0
65	75815.0
66	66706.0
67	57597.0
68	57597.0
69	49946.0
70	42295.0
71	36968.0
72	31641.0
73	25458.5
74	19276.0
75	19276.0
76	15152.5
77	11029.0
78	9069.0
79	7109.0
80	5603.5
81	4098.0
82	4098.0
83	3153.0
84	2208.0
85	1844.0
86	1480.0
87	1299.0
88	1118.0
89	1118.0
90	878.0
91	638.0
92	348.0
93	58.0
94	37.5
95	17.0
96	17.0
97	11.0
98	5.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2134973.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.05916875838688
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.67446729774653	50.59862822422511
2	9.063973760456694	10.70621511880805
3	2.271452162486317	4.024502297726467
4	0.8685597385791838	2.051856647099536
5	0.48458584607286037	1.4309618630571375
6	0.2938092087953071	1.04112765870061
7	0.20029178456220806	0.8280346413764541
8	0.14388863293809334	0.6798354444083552
9	0.10647240803116678	0.5659354722622056
>10	0.6888012147552975	8.046576233169683
>50	0.09911596527466653	4.115110438523161
>100	0.09632547712964558	11.296903211800108
>500	0.007145050822033398	2.8665153182069045
>1k	8.732839893596365E-4	0.7507975496090276
>5k	2.3816836073444633E-4	0.996999881027063
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	8490	0.397663108620109	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6802	0.31859887689446187	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5972	0.2797225070293629	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4055	0.18993214434093547	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.002154593992523559	0.0
3	0.0	0.0	0.0	0.0030445349894354635	0.0
4	0.0	0.0	0.0	0.00459022198407193	0.0
5	0.0	0.0	0.0	0.0049649339827716795	0.0
6	0.0	0.0	0.0	0.005152289982121553	0.0
7	0.0	0.0	0.0	0.005808035979846115	0.0
8	0.0	0.0	0.0	0.0060422309790334585	0.0
9	0.0	0.0	0.0	0.010117223964893233	0.0
10	0.0	0.0	0.0	0.014004860951403133	0.0
11	0.0	0.0	0.0	0.015737903945389473	0.0
12	0.0	0.0	0.0	0.016393649943114035	0.0
13	0.0	0.0	0.0	0.01756462493905075	0.0
14	0.0	0.0	0.0	0.018407726936125187	0.0
15	0.0	0.0	0.0	0.019812896931249248	0.0
16	0.0	0.0	0.0	0.02187381292409787	0.0
17	0.0	0.0	0.0	0.024777830914020926	0.0
18	0.0	0.0	0.0	0.026510873908007267	0.0
19	0.0	0.0	0.0	0.028712306900368294	0.0
20	0.0	0.0	0.0	0.03138212989110401	0.0
21	0.0	0.0	0.0	0.034754537879401755	0.0
22	0.0	0.0	0.0	0.04089044685811015	0.0
23	0.0	0.0	0.0	0.048853076830479826	0.0
24	0.0	0.0	0.0	0.05971972479277256	0.0
25	0.0	0.0	0.0	0.06379471777863233	0.0
26	0.0	0.0	0.0	0.07194470375035188	0.0
27	4.683899983746867E-5	0.0	0.0	0.0870268616980168	0.0
28	4.683899983746867E-5	0.0	0.0	0.12135984857888132	0.0
29	4.683899983746867E-5	0.0	0.0	0.16904195041342443	0.0
30	4.683899983746867E-5	0.0	0.0	0.2341481601875059	0.0
31	4.683899983746867E-5	0.0	0.0	0.4503101444374238	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCAATA	280	0.0	17.839285	36
GGTATCA	3985	0.0	17.45546	1
GACGGAC	295	0.0	16.305084	7
ACGGGAC	140	3.477362E-8	15.857142	3
ATTGCCG	475	0.0	15.578947	11
AGTCCGT	85	5.366826E-4	15.235294	6
TTTGCGC	280	0.0	15.196428	17
TTGCGCG	285	0.0	14.929826	18
AGTCGGT	310	0.0	14.919354	11
CAAGACG	350	0.0	14.8	4
GGTCTAG	150	8.110874E-8	14.8	1
ACGGACC	340	0.0	14.691176	8
CGGTCCA	380	0.0	14.118422	10
CGGACCA	370	0.0	14.0	9
TCTTATA	2090	0.0	13.985647	37
ATTTGCG	320	0.0	13.875001	16
TAGACAG	280	0.0	13.875	5
CCGGTCC	390	0.0	13.756411	9
TCTTGCG	365	0.0	13.684932	2
CGTAGAC	95	0.0012456859	13.631579	3
>>END_MODULE