FastQCFastQC Report
Fri 10 Feb 2017
ERR1630619.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630619.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1242244
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT28670.2307920183152424No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT24900.20044371315136156No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT20380.16405794674798188No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13470.10843280386139921No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11100.022.3333341
ATAATAC1451.546141E-1017.8620683
TAATACT1705.456968E-1217.4117644
TACACTC1107.808849E-716.8181825
TCACGTA1352.2195309E-816.44444525
ATAACGC1403.4733603E-815.8571423
TTACACT1403.4733603E-815.8571424
TCGCCAT3400.015.77941213
CTTATAC5200.015.65384637
ATCTCGT957.0604096E-515.57894837
GTATATA1201.9357085E-615.4166671
AACCGCG1353.9726E-715.0740757
GCTCGTA1001.0934763E-414.826
GTTATAG750.00410465514.81
CGCGGTC1252.9590265E-614.79999910
CGCTTCG1252.9590265E-614.79999932
GTATCAA16850.014.4925812
GTCGCCA3450.014.47826112
CGGACCA2700.014.3888899
ATCTCGC2850.014.28070211
Produced by FastQC (version 0.11.5)