##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630619.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1242244
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.34332546585051	31.0	30.0	31.0	27.0	34.0
2	30.53584480987632	31.0	30.0	33.0	27.0	34.0
3	30.62806179784326	31.0	30.0	33.0	27.0	34.0
4	34.42067902924063	35.0	35.0	37.0	30.0	37.0
5	33.45738035361813	35.0	33.0	37.0	28.0	37.0
6	33.65419112509298	35.0	33.0	37.0	29.0	37.0
7	33.69054791168241	35.0	33.0	37.0	29.0	37.0
8	33.659583785472094	35.0	33.0	37.0	29.0	37.0
9	35.10348208564501	37.0	34.0	39.0	30.0	39.0
10	34.65907985870731	37.0	33.0	39.0	28.0	39.0
11	34.99413883262869	37.0	33.0	39.0	29.0	39.0
12	34.817417512179574	37.0	33.0	39.0	29.0	39.0
13	34.99674218591517	37.0	33.0	39.0	29.0	39.0
14	35.81094937870499	38.0	34.0	40.0	29.0	40.0
15	35.76992281709551	38.0	34.0	40.0	29.0	40.0
16	35.724400359349694	38.0	34.0	40.0	29.0	40.0
17	35.63066032116074	38.0	34.0	40.0	29.0	40.0
18	35.55673523075982	38.0	33.0	40.0	28.0	40.0
19	35.57703639542634	38.0	34.0	40.0	27.0	40.0
20	35.53868966161237	38.0	34.0	40.0	27.0	40.0
21	35.44573368838972	38.0	34.0	40.0	27.0	40.0
22	35.37718193849196	38.0	34.0	39.0	27.0	40.0
23	35.3116907789452	37.0	34.0	39.0	27.0	40.0
24	35.22317193723616	37.0	33.0	39.0	27.0	40.0
25	35.12906160142452	37.0	33.0	39.0	27.0	40.0
26	34.81669865179465	37.0	33.0	39.0	27.0	40.0
27	34.66750895959248	37.0	33.0	39.0	26.0	40.0
28	34.51933597586304	37.0	33.0	39.0	25.0	40.0
29	34.34119222954589	37.0	32.0	39.0	25.0	40.0
30	34.226199522799064	37.0	32.0	39.0	25.0	40.0
31	34.16929524312454	37.0	32.0	39.0	25.0	40.0
32	34.05650983220688	37.0	32.0	39.0	24.0	40.0
33	34.00256551852937	37.0	32.0	39.0	24.0	40.0
34	33.91591667981491	36.0	32.0	39.0	24.0	40.0
35	33.84037757477597	36.0	31.0	39.0	24.0	40.0
36	33.77607136762182	36.0	31.0	39.0	24.0	40.0
37	33.547599344412205	36.0	31.0	39.0	24.0	40.0
38	33.389831627280955	36.0	31.0	39.0	23.0	40.0
39	33.32226197107815	36.0	31.0	39.0	23.0	40.0
40	33.20491867942208	36.0	31.0	39.0	23.0	40.0
41	33.03314083223586	36.0	30.0	39.0	22.0	40.0
42	32.95975106339817	36.0	30.0	39.0	22.0	40.0
43	32.00071161543143	35.0	29.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	3.0
11	3.0
12	5.0
13	14.0
14	31.0
15	63.0
16	119.0
17	242.0
18	452.0
19	838.0
20	1446.0
21	2446.0
22	4069.0
23	6142.0
24	9308.0
25	13670.0
26	19160.0
27	26059.0
28	34503.0
29	45305.0
30	58001.0
31	73103.0
32	91543.0
33	112708.0
34	137226.0
35	157643.0
36	163729.0
37	142134.0
38	100729.0
39	41547.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.28118147481493	21.505839432510843	12.834435102926639	24.378543989747588
2	19.533119097375394	22.75575490805349	34.097407594643244	23.613718399927873
3	21.244055113166173	24.81026271811335	29.540814847968676	24.404867320751798
4	16.269428550268707	16.62024529802519	34.29350433570216	32.816821816003944
5	14.06285721645667	38.193382298485645	34.25961405327777	13.484146431779909
6	34.30203728092066	36.02376022745934	15.51402139998261	14.16018109163739
7	28.31834969619495	31.326051886746885	21.975714915910242	18.379883501147923
8	26.38724759387045	34.90948638109743	20.206175276354728	18.497090748677394
9	26.497370886878908	14.276744343301315	20.112956874816863	39.11292789500291
10	16.410785642756174	27.611403234791233	33.613686200134595	22.364124922318
11	35.45414588438342	22.13993386162461	22.005902222107736	20.400018031884233
12	21.029604489939175	26.286623239878796	29.476576260380412	23.207196009801617
13	29.3513995640148	20.512395310422107	26.14421965410982	23.991985471453273
14	22.492843595944116	21.568548529918438	25.601572637903665	30.337035236233785
15	25.09305740257148	28.79973660569099	22.920376351183826	23.186829640553707
16	24.87120082689069	27.501038443333194	24.267535202424	23.360225527352114
17	23.37704991933952	27.350343410795304	25.71725039525246	23.55535627461272
18	22.881092603385486	26.004553050769413	27.497174468139914	23.617179877705187
19	23.89361510299104	26.23204459027373	27.27797437540451	22.59636593133072
20	24.36936704866355	25.359430192458166	27.255998016492732	23.015204742385553
21	24.182930245587823	26.443516732622573	26.078129578408106	23.295423443381495
22	24.1362405453357	25.933794005042486	26.518703249925135	23.41126219969668
23	23.470831817259732	26.20757274738296	26.825084282958905	23.4965111523984
24	24.29088005255006	26.426450842185595	26.35021783160152	22.932451273662824
25	24.24185586728533	25.82198022288697	26.68429068685379	23.25187322297391
26	23.878561699633888	26.518300752509166	26.43200530652593	23.171132241331012
27	24.411790276306427	25.790021928059222	26.03071538280724	23.76747241282711
28	23.512933046969838	26.08118855876945	26.98318526795058	23.42269312631013
29	23.349036099188243	26.539874614004976	26.88859837519843	23.222490911608347
30	23.228528372847844	27.030679963034636	26.694836119152114	23.045955544965402
31	23.80047720093637	26.49592189618143	26.48038549592512	23.223215406957088
32	23.37495693277649	26.249834976059454	26.50356934708479	23.871638744079263
33	23.363606505646235	26.055831221563558	26.965636380614438	23.614925892175773
34	23.871638744079263	25.98531367428621	26.68308319460589	23.459964387028634
35	24.024185264730598	25.992397628807222	27.01200408293379	22.971413023528388
36	23.51124255782278	26.456396649933506	26.60854067316888	23.423820119074836
37	24.236623400877765	25.44548413999182	26.69805609847985	23.619836360650563
38	23.550284807171536	25.791148920823932	27.221302739236414	23.437263532768117
39	23.775844359079215	25.756534143050803	26.99896316665647	23.46865833121351
40	23.310315847772255	25.400967925785917	27.752357829862735	23.536358396579093
41	22.764126854305594	25.906987677139114	27.923419231648534	23.405466236906758
42	23.67594450043631	25.372551608218675	27.539597695782792	23.411906195562224
43	23.182402168978076	25.09225240773954	27.57614446115256	24.149200962129825
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	149.0
1	142.5
2	136.0
3	296.5
4	457.0
5	457.0
6	675.0
7	893.0
8	914.0
9	935.0
10	1419.5
11	1904.0
12	1904.0
13	3116.5
14	4329.0
15	6326.0
16	8323.0
17	8615.0
18	8907.0
19	8907.0
20	11043.0
21	13179.0
22	14405.5
23	15632.0
24	17645.5
25	19659.0
26	19659.0
27	22182.0
28	24705.0
29	30821.0
30	36937.0
31	39629.5
32	42322.0
33	42322.0
34	46572.0
35	50822.0
36	54882.5
37	58943.0
38	65894.5
39	72846.0
40	72846.0
41	76491.0
42	80136.0
43	77700.5
44	75265.0
45	77990.0
46	80715.0
47	80715.0
48	82694.0
49	84673.0
50	86077.0
51	87481.0
52	91840.0
53	96199.0
54	96199.0
55	88037.0
56	79875.0
57	74728.0
58	69581.0
59	63615.0
60	57649.0
61	57649.0
62	53114.0
63	48579.0
64	43198.0
65	37817.0
66	32938.0
67	28059.0
68	28059.0
69	23789.5
70	19520.0
71	16981.0
72	14442.0
73	11662.5
74	8883.0
75	8883.0
76	6925.0
77	4967.0
78	4072.0
79	3177.0
80	2479.5
81	1782.0
82	1782.0
83	1359.0
84	936.0
85	777.5
86	619.0
87	548.5
88	478.0
89	478.0
90	373.5
91	269.0
92	157.5
93	46.0
94	29.5
95	13.0
96	13.0
97	8.5
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1242244.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.83536910220017
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.9525599966448	55.622497490803354
2	5.840281052387442	7.222718690699271
3	1.575559368054847	2.922758851983021
4	0.6936221901484515	1.7156153658122384
5	0.3902711700572925	1.2066280925220103
6	0.256784933858197	0.9527034699003398
7	0.17427044872071118	0.7543254264175855
8	0.12144270948070714	0.6007563812408632
9	0.09592635560579858	0.5338477444962116
>10	0.6757899608969165	8.344431882360723
>50	0.11145641439682369	4.870746729462527
>100	0.10678784384764795	12.41291879803396
>500	0.0038044780277943723	1.5231305057984492
>1k	0.0014430778726116584	1.3169205704693239
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2867	0.2307920183152424	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2490	0.20044371315136156	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2038	0.16405794674798188	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1347	0.10843280386139921	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.414984495799537E-4	0.0
3	0.0	0.0	0.0	2.414984495799537E-4	0.0
4	0.0	0.0	0.0	3.219979327732716E-4	0.0
5	0.0	8.04994831933179E-5	0.0	3.219979327732716E-4	0.0
6	0.0	8.04994831933179E-5	0.0	3.219979327732716E-4	0.0
7	0.0	8.04994831933179E-5	0.0	3.219979327732716E-4	0.0
8	0.0	8.04994831933179E-5	0.0	5.634963823532253E-4	0.0
9	8.04994831933179E-5	8.04994831933179E-5	0.0	0.0014489906974797222	0.0
10	8.04994831933179E-5	8.04994831933179E-5	0.0	0.002334485012606219	0.0
11	8.04994831933179E-5	8.04994831933179E-5	0.0	0.0025759834621861726	0.0
12	8.04994831933179E-5	8.04994831933179E-5	0.0	0.0029784808781527622	0.0
13	8.04994831933179E-5	8.04994831933179E-5	0.0	0.003219979327732716	0.0
14	8.04994831933179E-5	8.04994831933179E-5	0.0	0.0035419772605059874	0.0
15	8.04994831933179E-5	8.04994831933179E-5	0.0	0.003944474676472577	0.0
16	8.04994831933179E-5	8.04994831933179E-5	0.0	0.004668970025212438	0.0
17	8.04994831933179E-5	8.04994831933179E-5	0.0	0.005634963823532253	0.0
18	8.04994831933179E-5	8.04994831933179E-5	0.0	0.006439958655465432	0.0
19	8.04994831933179E-5	8.04994831933179E-5	0.0	0.0073254529705919285	0.0
20	8.04994831933179E-5	8.04994831933179E-5	0.0	0.008130447802525108	0.0
21	8.04994831933179E-5	8.04994831933179E-5	0.0	0.009257440567231559	0.0
22	8.04994831933179E-5	8.04994831933179E-5	0.0	0.011189428163871189	0.0
23	8.04994831933179E-5	8.04994831933179E-5	0.0	0.014328908008410586	0.0
24	8.04994831933179E-5	8.04994831933179E-5	0.0	0.018836879067236387	0.0
25	8.04994831933179E-5	8.04994831933179E-5	0.0	0.02060786769748938	0.0
26	8.04994831933179E-5	8.04994831933179E-5	0.0	0.022861853226902283	0.0
27	8.04994831933179E-5	8.04994831933179E-5	0.0	0.028496817050434536	0.0
28	8.04994831933179E-5	8.04994831933179E-5	0.0	0.042664726092458485	0.0
29	8.04994831933179E-5	8.04994831933179E-5	0.0	0.06335309327314119	0.0
30	8.04994831933179E-5	8.04994831933179E-5	0.0	0.09168891135718908	0.0
31	8.04994831933179E-5	8.04994831933179E-5	0.0	0.21066714751691293	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1110	0.0	22.333334	1
ATAATAC	145	1.546141E-10	17.862068	3
TAATACT	170	5.456968E-12	17.411764	4
TACACTC	110	7.808849E-7	16.818182	5
TCACGTA	135	2.2195309E-8	16.444445	25
ATAACGC	140	3.4733603E-8	15.857142	3
TTACACT	140	3.4733603E-8	15.857142	4
TCGCCAT	340	0.0	15.779412	13
CTTATAC	520	0.0	15.653846	37
ATCTCGT	95	7.0604096E-5	15.578948	37
GTATATA	120	1.9357085E-6	15.416667	1
AACCGCG	135	3.9726E-7	15.074075	7
GCTCGTA	100	1.0934763E-4	14.8	26
GTTATAG	75	0.004104655	14.8	1
CGCGGTC	125	2.9590265E-6	14.799999	10
CGCTTCG	125	2.9590265E-6	14.799999	32
GTATCAA	1685	0.0	14.492581	2
GTCGCCA	345	0.0	14.478261	12
CGGACCA	270	0.0	14.388889	9
ATCTCGC	285	0.0	14.280702	11
>>END_MODULE