FastQCFastQC Report
Fri 10 Feb 2017
ERR1630618.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630618.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2470035
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT52510.2125880807356981No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT52340.21189983137890758No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC46790.18943051414251216No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT41330.16732556421265285No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT35750.14473479120741203No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA33820.13692113674502587No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA32970.13347988996107343No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG28480.1153020098905481No Hit
GGGTACACTGGCTCCAGTGGGGCTCCCTGGGCACCCAGCAGTG27040.10947213298596982No Hit
CCTCTGCCTCTCCCTGCTGCTCCTGTCCACCTGCGTGGCTCTG25440.10299449198088285No Hit
AGTGTACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAG24990.10117265544820216No Hit
GCTGCATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTG24790.10036295032256627No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGA2350.018.89361816
CGTATGC3250.018.21538431
GGTATCA27850.017.6696591
GCCGTCT3400.017.41176436
ACGAACG2550.017.41176415
CTTATAC15700.016.96815337
TCTAGCG3100.016.11290228
TGCCGTC3600.015.93055535
ATGCCGT3650.015.71232934
TAACGAA2850.015.57894713
CAAGACG5550.015.3333334
AAGACGG5500.015.1363645
CTAGCGG3300.015.13636429
GCTTTAT11200.015.0312491
GTTCAAA6800.014.9632361
CTCGTAT4000.014.829
CCGATAA3000.014.89
CCAATAC2006.184564E-1114.83
GACGGAC5500.014.87
TCGTATG4000.014.830
Produced by FastQC (version 0.11.5)