FastQCFastQC Report
Fri 10 Feb 2017
ERR1630617.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630617.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences429515
Sequences flagged as poor quality0
Sequence length43
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT144363.3610001978976287No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT116392.7098005890364716No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT87772.043467632096667No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73911.720778086911982No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGT29450.6856570783325379No Hit
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT25070.5836815943564252TruSeq Adapter, Index 6 (95% over 21bp)
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20640.4805420066819552No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18660.43444350022700023No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT17660.4111614262598512No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA13370.31128132894078203No Hit
CATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTC13250.30848748006472415No Hit
ACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCC12880.299873112696879No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT12640.29428541494476324No Hit
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATG12490.2907931038496909No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG11820.2751941142917011No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11520.2682094921015564No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA9090.21163405236138436No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA9080.21140123162171287No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8620.20069147759682432No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA8140.1895160820925928No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA7280.16949349848084466No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7220.16809657404281572No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG6860.15971502741464208No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6730.15668835779891274No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA6540.15226476374515444No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA6180.1438832171169808No Hit
CTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTC5780.13457038753012118RNA PCR Primer, Index 6 (95% over 22bp)
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA5740.1336391045714352No Hit
GAACAGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAA5360.1247919164639186No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT5340.12432627498457562No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC5110.11897139797213137No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA4950.1152462661373875No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4920.11454780391837303No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC4910.11431498317870156No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA4680.1089601061662573No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA4670.1087272854265858No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA4540.10570061581085644No Hit
GTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAA4340.10104420101742662No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTAAC250.00549255929.5999981
CCGTCTT3750.027.13333137
GCCGTCT4000.025.43749836
AAACTCT400.001929228523.12500237
CGTCTTC903.810783E-922.6111137
TGCCGTC4750.021.42105335
TATGCCG4900.020.76530533
AGTGATC450.003821965820.5555558
CACTAAC450.003821965820.5555551
ATACACT450.003821965820.5555554
ATGCCGT5100.020.31372534
AAAACTC609.223979E-418.536
GGGCAGT5300.018.57
ACGGTGG609.223979E-418.58
ACTGCTC500.00702788618.4999988
GTACACG500.00702788618.4999984
GCAGTCA500.00702788618.49999823
TAAACGA500.00702788618.49999829
GAACAGA500.00702788618.4999981
TACACGG500.00702788618.4999985
Produced by FastQC (version 0.11.5)