##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630617.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	429515
Sequences flagged as poor quality	0
Sequence length	43
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.872470111637544	33.0	31.0	34.0	30.0	34.0
2	31.918095991990967	33.0	31.0	34.0	30.0	34.0
3	31.899363235276997	33.0	31.0	34.0	30.0	34.0
4	35.586999289896745	37.0	35.0	37.0	33.0	37.0
5	35.616488364783535	37.0	35.0	37.0	33.0	37.0
6	35.7321746621189	37.0	35.0	37.0	35.0	37.0
7	35.65316927231878	37.0	35.0	37.0	33.0	37.0
8	35.75652771148854	37.0	35.0	37.0	35.0	37.0
9	37.303253669836906	39.0	37.0	39.0	34.0	39.0
10	37.261215557081826	39.0	37.0	39.0	34.0	39.0
11	37.44200784605893	39.0	37.0	39.0	35.0	39.0
12	37.41702152427738	39.0	37.0	39.0	34.0	39.0
13	37.49205033584391	39.0	37.0	39.0	35.0	39.0
14	38.55878374445596	40.0	38.0	41.0	34.0	41.0
15	38.496571714608336	40.0	38.0	41.0	34.0	41.0
16	38.33831880143883	40.0	38.0	41.0	34.0	41.0
17	38.493258675482814	40.0	38.0	41.0	34.0	41.0
18	38.5990803580783	40.0	38.0	41.0	34.0	41.0
19	38.70124442685354	40.0	38.0	41.0	34.0	41.0
20	38.73114326624216	40.0	38.0	41.0	35.0	41.0
21	38.659855883962145	40.0	38.0	41.0	35.0	41.0
22	38.52881040243065	40.0	38.0	41.0	34.0	41.0
23	38.458014271911345	40.0	38.0	41.0	34.0	41.0
24	38.3435828783628	40.0	38.0	41.0	34.0	41.0
25	38.22680465175838	40.0	37.0	41.0	34.0	41.0
26	37.80902180366227	40.0	37.0	41.0	33.0	41.0
27	37.54757109763338	39.0	35.0	41.0	33.0	41.0
28	37.34318941131276	39.0	35.0	41.0	33.0	41.0
29	37.07787155279792	39.0	35.0	41.0	32.0	41.0
30	36.78069450426644	39.0	35.0	41.0	32.0	41.0
31	36.407545720172756	38.0	35.0	40.0	31.0	41.0
32	36.04768867210691	38.0	35.0	40.0	31.0	41.0
33	35.72045213787644	37.0	35.0	40.0	30.0	41.0
34	35.4219689649954	37.0	35.0	40.0	29.0	41.0
35	35.08962667194394	37.0	35.0	40.0	27.0	41.0
36	34.73843288360127	37.0	35.0	40.0	24.0	41.0
37	34.2082209003178	36.0	34.0	40.0	21.0	41.0
38	33.73889386866582	35.0	33.0	40.0	19.0	41.0
39	33.23797306264042	35.0	33.0	40.0	15.0	41.0
40	32.67175535196675	35.0	33.0	40.0	12.0	41.0
41	32.09895114256778	35.0	32.0	40.0	10.0	41.0
42	31.52701069811299	35.0	31.0	40.0	8.0	41.0
43	30.54005564415678	35.0	27.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	6.0
11	12.0
12	8.0
13	4.0
14	8.0
15	4.0
16	7.0
17	13.0
18	34.0
19	69.0
20	140.0
21	336.0
22	530.0
23	955.0
24	1560.0
25	2486.0
26	3754.0
27	5423.0
28	7342.0
29	9725.0
30	12051.0
31	14400.0
32	17179.0
33	21316.0
34	26168.0
35	31114.0
36	42352.0
37	72981.0
38	78209.0
39	81326.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.946369742616675	20.382291654540584	13.774839062663702	16.89649954017904
2	17.09835512147422	25.26105025435666	32.80490786119227	24.835686762976845
3	24.60868654179714	28.596672991630097	29.72399101311945	17.070649453453314
4	13.770881110089286	18.25780240503824	29.16056482311444	38.81075166175803
5	10.845488516117015	44.78656158690616	29.841798307393226	14.5261515895836
6	29.9742733082663	29.63970990535837	19.186989977067157	21.199026809308172
7	24.005680826047985	37.21523113278931	21.573868200179273	17.205219840983435
8	33.83537245497829	29.42784303225731	20.063094420450973	16.67369009231342
9	28.676064863858073	11.453150646659605	23.783802661141053	36.086981828341266
10	21.121031861518226	31.890620816502334	25.954157596358684	21.03418972562076
11	26.84283435968476	25.810972841460718	19.636566825372803	27.709625973481717
12	19.750648987811832	33.878677112557185	28.22881622294914	18.141857676681838
13	30.058088774548036	20.32385364888304	31.101591329755657	18.516466246813266
14	22.93144593320373	24.557465979069416	31.91250596603145	20.5985821216954
15	28.695156164511133	24.29368008102162	29.56846675901889	17.442696995448355
16	20.90986345063618	25.26733641432779	33.76273238420078	20.060067750835245
17	17.097423838515535	32.40003259490356	31.92228443709766	18.580259129483252
18	16.53562739368823	24.146304552809564	36.54610432697344	22.77196372652876
19	17.088111008928674	28.71122079554847	38.7979465210761	15.40272167444676
20	18.716459262191076	24.15352199573938	40.238874078902946	16.891144663166596
21	20.885417272970678	25.33625135327055	37.54839761125921	16.229933762499563
22	19.745061290059716	26.73014912168376	36.86669848550109	16.658091102755435
23	17.861541506117366	27.30568199015168	36.56822229724224	18.264554206488715
24	16.557978184696694	29.544486222832727	37.48879550190331	16.408740090567267
25	16.459727832555323	28.8481193904753	37.1279233554125	17.564229421556874
26	17.20894497281818	28.750567500552947	37.492287812998384	16.548199713630492
27	16.494418122766376	30.223391499714797	36.638301339883355	16.643889037635475
28	15.352199573938046	29.644133499412128	38.57350732803278	16.430159598617045
29	15.11542088169214	30.787748972678486	38.1111253390452	15.98570480658417
30	15.1761870947464	30.988906091754654	38.984668754292635	14.850238059206314
31	14.352234497048997	32.6822113313854	36.76914659557873	16.196407575986868
32	14.23396156129588	32.44915777097424	38.17864335354994	15.138237314179948
33	13.833277068321244	31.87013259141124	38.271771649418525	16.02481869084898
34	14.513579269641339	33.66518049427843	35.62879061266778	16.192449623412454
35	14.808330326065446	32.63262051383537	36.48743350057623	16.07161565952295
36	14.423943284867816	32.86590689498621	35.32612365109484	17.38402616905114
37	14.24304157014307	33.2309698147911	35.12450089053933	17.4014877245265
38	14.56968906790217	31.384934169935857	36.10514184603564	17.94023491612633
39	15.365703176838993	32.0370650617557	35.114722419473125	17.48250934193218
40	14.147817887617428	31.848713083361467	36.19896860412325	17.804500424897853
41	14.004400311979792	32.60118971397972	34.42859969966124	18.96581027437924
42	14.068658836129122	32.23868782231121	35.32053595334273	18.372117388216942
43	13.674726144604962	32.72272214008824	34.70728612504802	18.89526559025878
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	78.0
1	144.0
2	210.0
3	877.5
4	1545.0
5	1545.0
6	2442.5
7	3340.0
8	3600.5
9	3861.0
10	5629.5
11	7398.0
12	7398.0
13	13244.5
14	19091.0
15	25196.5
16	31302.0
17	28126.0
18	24950.0
19	24950.0
20	27407.5
21	29865.0
22	21505.0
23	13145.0
24	11703.5
25	10262.0
26	10262.0
27	10728.0
28	11194.0
29	11321.5
30	11449.0
31	11651.5
32	11854.0
33	11854.0
34	11930.5
35	12007.0
36	11902.5
37	11798.0
38	13341.5
39	14885.0
40	14885.0
41	15986.0
42	17087.0
43	18611.0
44	20135.0
45	21669.5
46	23204.0
47	23204.0
48	25988.5
49	28773.0
50	28228.5
51	27684.0
52	21562.5
53	15441.0
54	15441.0
55	16082.0
56	16723.0
57	15171.0
58	13619.0
59	11952.5
60	10286.0
61	10286.0
62	9714.5
63	9143.0
64	8477.5
65	7812.0
66	7086.0
67	6360.0
68	6360.0
69	5769.5
70	5179.0
71	4557.5
72	3936.0
73	3291.5
74	2647.0
75	2647.0
76	2083.5
77	1520.0
78	1220.0
79	920.0
80	686.0
81	452.0
82	452.0
83	334.5
84	217.0
85	149.0
86	81.0
87	58.5
88	36.0
89	36.0
90	28.5
91	21.0
92	12.0
93	3.0
94	2.5
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	429515.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.47457801496139
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.47088206103116	32.96021297958033
2	10.666907524915837	8.848109766410012
3	3.4771617466053337	4.32641448390667
4	1.6669958566581582	2.7655179883034475
5	0.9702438114050425	2.012022632482596
6	0.6525389862112966	1.623826745485454
7	0.47204615917503134	1.3704540678757404
8	0.3269235153164322	1.0847211872733435
9	0.26751818847740555	0.9985683580584572
>10	1.7365199131739153	13.85996439669526
>50	0.16386957798817092	4.720593584517262
>100	0.11093576241810883	8.498096089760791
>500	0.00844688546330778	2.4072411476162388
>1k	0.006757508370646224	4.658234664713873
>5k	0.001126251395107704	3.776101181203003
>10k+	0.001126251395107704	6.089920726117535
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	14436	3.3610001978976287	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	11639	2.7098005890364716	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	8777	2.043467632096667	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7391	1.720778086911982	No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGT	2945	0.6856570783325379	No Hit
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT	2507	0.5836815943564252	TruSeq Adapter, Index 6 (95% over 21bp)
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2064	0.4805420066819552	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1866	0.43444350022700023	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1766	0.4111614262598512	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	1337	0.31128132894078203	No Hit
CATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTC	1325	0.30848748006472415	No Hit
ACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCC	1288	0.299873112696879	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	1264	0.29428541494476324	No Hit
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATG	1249	0.2907931038496909	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	1182	0.2751941142917011	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1152	0.2682094921015564	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	909	0.21163405236138436	No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	908	0.21140123162171287	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	862	0.20069147759682432	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	814	0.1895160820925928	No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA	728	0.16949349848084466	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	722	0.16809657404281572	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	686	0.15971502741464208	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	673	0.15668835779891274	No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	654	0.15226476374515444	No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	618	0.1438832171169808	No Hit
CTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTC	578	0.13457038753012118	RNA PCR Primer, Index 6 (95% over 22bp)
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA	574	0.1336391045714352	No Hit
GAACAGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAA	536	0.1247919164639186	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT	534	0.12432627498457562	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	511	0.11897139797213137	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	495	0.1152462661373875	No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	492	0.11454780391837303	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC	491	0.11431498317870156	No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA	468	0.1089601061662573	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	467	0.1087272854265858	No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA	454	0.10570061581085644	No Hit
GTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAA	434	0.10104420101742662	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	4.656414793429799E-4	0.0
3	0.0	0.0	0.0	9.312829586859598E-4	0.0
4	0.0	0.0	0.0	0.0013969244380289396	0.0
5	2.3282073967148995E-4	0.0	0.0	0.0016297451777004296	0.0
6	2.3282073967148995E-4	0.0	0.0	0.0016297451777004296	0.0
7	2.3282073967148995E-4	0.0	0.0	0.0016297451777004296	0.0
8	2.3282073967148995E-4	0.0	0.0	0.0016297451777004296	0.0
9	2.3282073967148995E-4	0.0	0.0	0.0027938488760578793	0.0
10	2.3282073967148995E-4	0.0	0.0	0.004190773314086819	0.0
11	4.656414793429799E-4	0.0	0.0	0.0055876977521157585	0.0
12	4.656414793429799E-4	0.0	0.0	0.010011291805874067	0.0
13	4.656414793429799E-4	0.0	0.0	0.010244112545545558	0.0
14	4.656414793429799E-4	0.0	0.0	0.014202065119960887	0.0
15	4.656414793429799E-4	0.0	0.0	0.021419508049777075	0.0
16	4.656414793429799E-4	0.0	0.0	0.03678567686809541	0.0
17	4.656414793429799E-4	0.0	0.0	0.06169749601294483	0.0
18	4.656414793429799E-4	0.0	0.0	0.07007904264111847	0.0
19	4.656414793429799E-4	0.0	0.0	0.0831170040627219	0.0
20	4.656414793429799E-4	0.0	0.0	0.09429239956695343	0.0
21	4.656414793429799E-4	0.0	0.0	0.1206011431498318	0.0
22	4.656414793429799E-4	0.0	0.0	0.16832939478248723	0.0
23	4.656414793429799E-4	0.0	0.0	0.23049253227477504	0.0
24	4.656414793429799E-4	0.0	0.0	0.3427121287964332	0.0
25	4.656414793429799E-4	0.0	0.0	0.3676239479412826	0.0
26	4.656414793429799E-4	0.0	0.0	0.4232681047227687	0.0
27	4.656414793429799E-4	0.0	0.0	0.47402302597115353	0.0
28	4.656414793429799E-4	0.0	0.0	0.544567710091615	0.0
29	4.656414793429799E-4	0.0	0.0	0.6404898548362689	0.0
30	4.656414793429799E-4	0.0	0.0	0.8130100229328429	0.0
31	4.656414793429799E-4	0.0	0.0	1.3140402547058891	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGTAAC	25	0.005492559	29.599998	1
CCGTCTT	375	0.0	27.133331	37
GCCGTCT	400	0.0	25.437498	36
AAACTCT	40	0.0019292285	23.125002	37
CGTCTTC	90	3.810783E-9	22.61111	37
TGCCGTC	475	0.0	21.421053	35
TATGCCG	490	0.0	20.765305	33
AGTGATC	45	0.0038219658	20.555555	8
CACTAAC	45	0.0038219658	20.555555	1
ATACACT	45	0.0038219658	20.555555	4
ATGCCGT	510	0.0	20.313725	34
AAAACTC	60	9.223979E-4	18.5	36
GGGCAGT	530	0.0	18.5	7
ACGGTGG	60	9.223979E-4	18.5	8
ACTGCTC	50	0.007027886	18.499998	8
GTACACG	50	0.007027886	18.499998	4
GCAGTCA	50	0.007027886	18.499998	23
TAAACGA	50	0.007027886	18.499998	29
GAACAGA	50	0.007027886	18.499998	1
TACACGG	50	0.007027886	18.499998	5
>>END_MODULE