FastQCFastQC Report
Fri 10 Feb 2017
ERR1630616.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630616.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1874737
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT70330.3751459538057872No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT68680.3663447192859585No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT53380.2847332719202747No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37170.1982678103648672No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC701.21971854E-418.53
TCGTATA500.00703649718.52
GGTATCA34300.018.230321
AGTCGGT3250.017.07692311
GCAGTCG3500.016.9142869
CAGTCGG3500.015.85714310
GACGGAC3500.015.3285727
GCGTTAT2550.015.2352941
AATTTCG2006.184564E-1114.828
TAACGCT750.0041056914.832
CGGTGAT3900.014.70512914
CGTTATT2550.014.5098042
CGCCTTA3200.014.45312525
TCGGTGA3850.014.41558413
CTTATAC13300.014.18796937
GTATTAG4600.014.0760861
CGCATCG2252.7284841E-1113.977777513
CGTATGC1203.3025415E-513.87500131
CTTAACG950.001245586713.63157930
TAGCGCA950.001245586713.6315794
Produced by FastQC (version 0.11.5)