##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630616.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1874737
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.987631865162953	33.0	31.0	34.0	30.0	34.0
2	32.15297239026061	33.0	31.0	34.0	30.0	34.0
3	32.260592285744615	34.0	31.0	34.0	30.0	34.0
4	35.8460776098194	37.0	35.0	37.0	35.0	37.0
5	35.82283274934031	37.0	35.0	37.0	35.0	37.0
6	35.85729518327104	37.0	35.0	37.0	35.0	37.0
7	35.82995214795462	37.0	35.0	37.0	35.0	37.0
8	35.8256080719589	37.0	35.0	37.0	35.0	37.0
9	37.55545337826052	39.0	37.0	39.0	35.0	39.0
10	37.45634134281235	39.0	37.0	39.0	34.0	39.0
11	37.56528835778032	39.0	37.0	39.0	35.0	39.0
12	37.49028850446756	39.0	37.0	39.0	35.0	39.0
13	37.545755484635976	39.0	37.0	39.0	35.0	39.0
14	38.813434631097586	40.0	38.0	41.0	35.0	41.0
15	38.78797079270319	40.0	38.0	41.0	35.0	41.0
16	38.7678394356115	40.0	38.0	41.0	35.0	41.0
17	38.75901366431665	40.0	38.0	41.0	35.0	41.0
18	38.75142326630349	40.0	38.0	41.0	35.0	41.0
19	38.78865142150606	40.0	38.0	41.0	35.0	41.0
20	38.76929990713364	40.0	38.0	41.0	35.0	41.0
21	38.697582647592704	40.0	38.0	41.0	34.0	41.0
22	38.63914085015658	40.0	38.0	41.0	34.0	41.0
23	38.61315427177252	40.0	38.0	41.0	34.0	41.0
24	38.56606500005067	40.0	38.0	41.0	34.0	41.0
25	38.523633981726505	40.0	38.0	41.0	34.0	41.0
26	38.33359932619882	40.0	38.0	41.0	34.0	41.0
27	38.21963080688118	40.0	38.0	41.0	34.0	41.0
28	38.127671241352786	40.0	38.0	41.0	33.0	41.0
29	38.02010468668405	40.0	37.0	41.0	33.0	41.0
30	37.94685121166329	40.0	37.0	41.0	33.0	41.0
31	37.8976923163089	40.0	37.0	41.0	33.0	41.0
32	37.79794819219976	40.0	37.0	41.0	33.0	41.0
33	37.760783512567365	40.0	37.0	41.0	33.0	41.0
34	37.67675412604542	40.0	37.0	41.0	33.0	41.0
35	37.62627717914566	40.0	37.0	41.0	33.0	41.0
36	37.58296603736951	40.0	37.0	41.0	33.0	41.0
37	37.395539214300456	40.0	36.0	41.0	32.0	41.0
38	37.28575901579795	40.0	36.0	41.0	31.0	41.0
39	37.22137558494872	40.0	36.0	41.0	31.0	41.0
40	37.11631018110807	40.0	36.0	41.0	31.0	41.0
41	36.993470017394436	39.0	36.0	41.0	31.0	41.0
42	36.903729963189505	39.0	36.0	41.0	31.0	41.0
43	36.084988987788684	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	5.0
11	3.0
12	4.0
13	2.0
14	1.0
15	5.0
16	11.0
17	36.0
18	48.0
19	119.0
20	283.0
21	543.0
22	946.0
23	1652.0
24	2731.0
25	4456.0
26	6716.0
27	10104.0
28	14773.0
29	20481.0
30	27576.0
31	36324.0
32	46319.0
33	60196.0
34	79391.0
35	106682.0
36	147596.0
37	225755.0
38	409714.0
39	672262.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.67839328929871	19.303667661117267	12.162879379881018	24.855059669703003
2	18.60831679323553	20.916587233302593	35.178481034939836	25.296614938522044
3	20.174083084720685	23.629341075574867	29.36278528668288	26.83379055302157
4	14.120007233014551	15.549167696588908	34.63973880069578	35.69108626970076
5	14.14934468141398	37.42914339451347	34.23520205767529	14.18630986639726
6	34.70945524625588	34.81016270548882	15.867985749467792	14.61239629878751
7	28.798759506000042	30.81322873555064	21.67461355912856	18.713398199320757
8	27.188933701100478	33.56310778525201	20.115354847106552	19.13260366654096
9	26.65563223001413	13.601907894280638	20.3505878424547	39.39187203325053
10	16.904717835088334	26.86382143201953	33.297577206829544	22.93388352606259
11	35.26601331280067	21.870321010360385	21.783375481467534	21.080290195371404
12	21.439380563780414	25.930997254548238	29.563026707212796	23.066595474458552
13	29.71184758182081	20.138771465010826	25.82836952596551	24.321011427202855
14	22.698917234790798	21.326351376219705	25.967215668117717	30.00751572087178
15	25.59468341426024	27.921356435596035	23.020562350879086	23.463397799264644
16	24.874635748907714	26.68240931928052	24.68660937507501	23.756345556736758
17	23.186772331265665	26.807920257614803	26.109262259186224	23.89604515193331
18	23.059501146027415	25.359823804618998	27.742131296283155	23.838543753070432
19	23.524953100088172	25.848905739845108	27.589469882975585	23.036671277091134
20	24.156134967198064	25.101494236258205	27.50252435408273	23.239846442460994
21	24.586755368886408	25.51344535260146	26.69990510668963	23.199894171822503
22	24.364430850834008	25.40196304868363	26.837311046829505	23.39629505365286
23	23.621019908392483	25.493815932581477	27.14876806720089	23.736396091825146
24	23.59904349250055	26.024183658827877	26.802586176087633	23.57418667258394
25	23.888684119425818	25.557184821124242	26.91316168614584	23.640969373304095
26	23.53306090400947	26.262777125538143	26.931670949045117	23.27249102140727
27	24.01259483330195	25.635862523649983	26.50339754322873	23.848145099819334
28	23.23275211402986	25.78484342070381	27.409444631433637	23.572959833832694
29	22.974475886484345	26.151134799174496	27.574961181221685	23.299428133119473
30	22.91724119169782	26.63168220395714	27.33727450837104	23.113802095973995
31	23.4632911176341	26.42818699369565	26.69046378238654	23.418058106283706
32	22.773220990464264	26.140039909597984	26.963568756577587	24.12317034336016
33	22.841230529935665	25.814874299701774	27.51127224778729	23.832622922575272
34	23.554450570933415	25.53227466039236	27.272465417815937	23.64080935085828
35	23.39005417826607	25.643490260233836	27.71226043973101	23.25419512176908
36	22.813653328440203	26.134332442363917	27.17735874418652	23.874655485009363
37	23.30694918807278	25.244500962001602	27.442249232825723	24.00630061709989
38	22.67923447395555	25.13253859074633	28.18507342629926	24.00315350899886
39	22.908706661254353	25.046073129190923	28.09972812186456	23.94549208769017
40	22.29267358568162	25.08773230591811	28.768888649447895	23.850705458952376
41	21.77030698172597	25.13776599064295	29.182066604542396	23.909860423088677
42	22.21346247500316	25.167743528825643	28.944646635768112	23.674147360403087
43	21.687575377239583	24.64836401052521	29.039913331843348	24.624147280391863
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	405.0
1	364.5
2	324.0
3	869.5
4	1415.0
5	1415.0
6	1924.5
7	2434.0
8	2399.5
9	2365.0
10	3462.0
11	4559.0
12	4559.0
13	7538.0
14	10517.0
15	14204.0
16	17891.0
17	17578.0
18	17265.0
19	17265.0
20	20202.5
21	23140.0
22	22408.0
23	21676.0
24	24678.0
25	27680.0
26	27680.0
27	31682.0
28	35684.0
29	41787.0
30	47890.0
31	53985.5
32	60081.0
33	60081.0
34	66902.5
35	73724.0
36	80297.0
37	86870.0
38	94774.0
39	102678.0
40	102678.0
41	107478.5
42	112279.0
43	113896.5
44	115514.0
45	117531.5
46	119549.0
47	119549.0
48	122624.5
49	125700.0
50	126832.5
51	127965.0
52	129595.5
53	131226.0
54	131226.0
55	126075.5
56	120925.0
57	114998.5
58	109072.0
59	102045.5
60	95019.0
61	95019.0
62	88024.0
63	81029.0
64	71716.0
65	62403.0
66	54414.5
67	46426.0
68	46426.0
69	39702.0
70	32978.0
71	28457.5
72	23937.0
73	18894.0
74	13851.0
75	13851.0
76	10904.5
77	7958.0
78	6475.5
79	4993.0
80	3986.5
81	2980.0
82	2980.0
83	2285.5
84	1591.0
85	1400.5
86	1210.0
87	1076.0
88	942.0
89	942.0
90	726.5
91	511.0
92	283.5
93	56.0
94	37.0
95	18.0
96	18.0
97	12.5
98	7.0
99	3.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1874737.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.74778967068768
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.80093027767934	54.4656651628385
2	8.209437002100458	10.302480526451209
3	2.0317667653644245	3.8246662095894104
4	0.87052448194077	2.1849394838401515
5	0.47984005334422125	1.5054451371407378
6	0.2920021545458727	1.0993493866099235
7	0.20175814045467608	0.8861914153119356
8	0.14327365831786548	0.7192084301983518
9	0.10617838200682893	0.5996212903564587
>10	0.6974320458221841	8.664199716381507
>50	0.09262425476350945	4.0345759265720735
>100	0.06954589534615424	8.487202011641633
>500	0.003919942953247873	1.674933475527072
>1k	5.112969069453748E-4	0.5227884238995077
>5k	2.556484534726874E-4	1.0287334036414677
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7033	0.3751459538057872	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6868	0.3663447192859585	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5338	0.2847332719202747	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3717	0.1982678103648672	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	5.334081527168877E-4	0.0
3	0.0	0.0	0.0	6.934305985319541E-4	0.0
4	0.0	0.0	0.0	9.60134674890398E-4	0.0
5	0.0	0.0	0.0	9.60134674890398E-4	0.0
6	0.0	0.0	0.0	0.0010134754901620867	0.0
7	0.0	0.0	0.0	0.0010668163054337754	0.0
8	0.0	0.0	0.0	0.0012268387512488418	0.0
9	0.0	0.0	0.0	0.00218697342613924	0.0
10	0.0	5.3340815271688776E-5	0.0	0.002880404024671194	0.0
11	0.0	1.0668163054337755E-4	0.0	0.003573834623203148	0.0
12	0.0	1.0668163054337755E-4	0.0	0.00394722033010497	0.0
13	0.0	1.0668163054337755E-4	0.0	0.004107242775920035	0.0
14	0.0	1.0668163054337755E-4	0.0	0.004747332559180301	0.0
15	0.0	1.0668163054337755E-4	0.0	0.005974171310429143	0.0
16	0.0	1.0668163054337755E-4	0.0	0.00832116718238345	0.0
17	0.0	1.0668163054337755E-4	0.0	0.01136159365286971	0.0
18	0.0	1.0668163054337755E-4	0.0	0.011841660990314909	0.0
19	0.0	1.0668163054337755E-4	0.0	0.014828746645529479	0.0
20	0.0	1.0668163054337755E-4	0.0	0.016375630288408455	0.0
21	0.0	1.0668163054337755E-4	0.0	0.0204295322490568	0.0
22	0.0	1.0668163054337755E-4	0.0	0.028430654539810116	0.0
23	0.0	1.0668163054337755E-4	0.0	0.03941886248577801	0.0
24	0.0	1.0668163054337755E-4	0.0	0.05835485190722752	0.0
25	0.0	1.0668163054337755E-4	0.0	0.06230207223733249	0.0
26	0.0	1.0668163054337755E-4	0.0	0.06971644556009723	0.0
27	0.0	1.0668163054337755E-4	0.0	0.08331835345437787	0.0
28	0.0	1.0668163054337755E-4	0.0	0.11900335887113765	0.0
29	0.0	1.0668163054337755E-4	0.0	0.17127735783739265	0.0
30	0.0	1.0668163054337755E-4	0.0	0.2537955990626952	0.0
31	0.0	1.0668163054337755E-4	0.0	0.5420493647909014	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATAC	70	1.21971854E-4	18.5	3
TCGTATA	50	0.007036497	18.5	2
GGTATCA	3430	0.0	18.23032	1
AGTCGGT	325	0.0	17.076923	11
GCAGTCG	350	0.0	16.914286	9
CAGTCGG	350	0.0	15.857143	10
GACGGAC	350	0.0	15.328572	7
GCGTTAT	255	0.0	15.235294	1
AATTTCG	200	6.184564E-11	14.8	28
TAACGCT	75	0.00410569	14.8	32
CGGTGAT	390	0.0	14.705129	14
CGTTATT	255	0.0	14.509804	2
CGCCTTA	320	0.0	14.453125	25
TCGGTGA	385	0.0	14.415584	13
CTTATAC	1330	0.0	14.187969	37
GTATTAG	460	0.0	14.076086	1
CGCATCG	225	2.7284841E-11	13.9777775	13
CGTATGC	120	3.3025415E-5	13.875001	31
CTTAACG	95	0.0012455867	13.631579	30
TAGCGCA	95	0.0012455867	13.631579	4
>>END_MODULE