FastQCFastQC Report
Fri 10 Feb 2017
ERR1630615.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630615.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1974980
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT60890.3083069195637424No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT46960.23777455974237716No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT44000.2227870661981387No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27810.14081155252205085No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31600.018.909811
TCTAGCG2750.018.83636328
CACGAGT609.240017E-418.54
TAGTTCG701.2197603E-418.59
CGAACGA1851.8189894E-1217.016
AAGACGG4350.016.5862085
CGGTGAT4150.016.49397714
GACGGAC3950.016.3924057
CGCAATA3050.016.3770536
CTAGCGG2850.016.2280729
CGAATTA700.00259337815.85714215
ACGAACG2003.6379788E-1215.72515
CAAGACG4450.014.9662934
CGGACCA4350.014.8850589
AGACGGA4450.014.5505636
GTAAACG2700.014.38888827
ACGGACC4400.014.2954548
TCGGTGA4850.014.11340213
CAGTCGG4750.014.02105210
GTCGGTG5150.014.00970812
Produced by FastQC (version 0.11.5)