FastQCFastQC Report
Fri 10 Feb 2017
ERR1630614.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630614.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences345093
Sequences flagged as poor quality0
Sequence length43
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT118673.4387831685951324No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT111043.217683349126178No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT78682.2799651108541754No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52811.5303121187621889No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGT38271.1089764208488726No Hit
TCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT31580.9151156354953591RNA PCR Primer, Index 41 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCC15840.45900670254105413No Hit
CATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTC15570.4511827246568316No Hit
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATG15400.4462565163593582No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14730.42684146012813934No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT14120.40916506564897No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13010.3769998232360552No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA10510.30455558356732826No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG8590.2489184075017459No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8040.23298067477462597No Hit
CTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTC7840.22718513560112782RNA PCR Primer, Index 41 (95% over 23bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT7360.21327584158473226No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6830.1979176627749621No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA6580.19067323880808942No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA6530.18922435401471488No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA6140.17792305262639346No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA5430.157348888560475No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG5410.1567693346431252No Hit
GAACAGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAA5330.15445111897372593No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5290.1532920111390263No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5160.1495249106762525No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA5100.14778624892420306No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA4830.13996227103998052No Hit
CACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCG4810.1393827171226307No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA4710.13648494753588164No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4620.13387695490780746No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA4300.12460409223021042No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC4260.12344498439551077No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA3790.10982546733779011No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC3470.10055260466019306No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTGAG200.001840151737.011
CTCCGGT303.5943047E-430.8333347
AGGTCAT250.00549108129.5999987
ACCGAGC250.00549108129.5999988
TTTCCGC250.00549108129.59999817
TGGATGT250.00549108129.5999984
TAAGGTC250.00549108129.5999985
ACGTAGG250.00549108129.59999827
CACGTAG250.00549108129.59999826
TCAGATC358.856768E-426.42857220
ACTCCGG358.856768E-426.4285726
CTGATCA358.856768E-426.4285724
TATACAC6500.026.1846163
CCGTCTT4950.026.16161737
CGTCTTC1700.025.02941137
GCCGTCT5100.025.02941136
TTGAGCT451.3205921E-424.6666664
TACACAT7050.024.1418465
ATACACA7300.023.315074
TTATACA7350.023.1564642
Produced by FastQC (version 0.11.5)