FastQCFastQC Report
Fri 10 Feb 2017
ERR1630613.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630613.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1968301
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT39450.20042666238547865No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT37300.1895035362985641No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT29530.15002786667283105No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG500.00703662118.4999985
CGTAGGT752.0682093E-417.26666627
CGAATTA1005.8820733E-616.6515
GGACCGT1005.8820733E-616.656
CGAACGA1951.8189894E-1216.12820416
GGTATCA22800.016.0657881
CAGTCGG4500.016.03333310
TACGTCT700.002593373415.8571434
TCGTTTA2400.015.41666630
CGAACTA1201.9371073E-615.41666624
ACGAACG2055.456968E-1215.34146315
AGTCGGT4600.015.28260911
GCAGTCG4900.015.1020419
TCTTATA14100.015.08865337
TCGGTGA5350.014.8691613
ATACCGT2251.8189894E-1214.86
TCGCACG2550.014.50980422
CCGCTTA1152.2113158E-514.47826125
TACACCA6050.014.3719015
TAACGAA2201.8189894E-1114.29545413
Produced by FastQC (version 0.11.5)