FastQCFastQC Report
Fri 10 Feb 2017
ERR1630612.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630612.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1168933
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT64590.5525551934969755No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT57330.4904472711438551No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT50750.4341566197549389No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35280.30181370531929547No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACAG1251.8189894E-1120.725
CTAGACA1603.45608E-1117.343754
GGTATCA31900.017.3401241
TCTGTCG1151.2421624E-616.0869568
AAGACGG2750.015.4727285
CGAACGA1201.9354702E-615.41666716
CGCAAGA2650.015.3584912
AAGACCG855.3642463E-415.2352945
ACGGACC2550.015.2352948
CGCAATA1353.972018E-715.07407536
CGAGTTC750.00410446114.814
TGTCGCC1508.100142E-814.810
ATCTGCG1752.237357E-914.836
CGGAACC2059.276846E-1114.43902414
TTTGCCG908.274165E-414.3888889
CATACTC2600.014.2307693
ATAACGA1304.4438293E-614.23076912
ACGAACG1304.4438293E-614.23076915
CCCTAGA1051.6556689E-414.0952381
ATTCCGT2101.364242E-1014.09523829
Produced by FastQC (version 0.11.5)