##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630612.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1168933
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.599231093655497	31.0	31.0	34.0	30.0	34.0
2	31.769299010294002	31.0	31.0	34.0	30.0	34.0
3	31.86592131456636	33.0	31.0	34.0	30.0	34.0
4	35.5296419897462	37.0	35.0	37.0	33.0	37.0
5	35.480852195977015	37.0	35.0	37.0	33.0	37.0
6	35.51098908149569	37.0	35.0	37.0	33.0	37.0
7	35.49280069944128	37.0	35.0	37.0	33.0	37.0
8	35.48747190814187	37.0	35.0	37.0	33.0	37.0
9	37.127931198794116	39.0	37.0	39.0	33.0	39.0
10	36.981300040293156	39.0	37.0	39.0	33.0	39.0
11	37.12238425983354	39.0	37.0	39.0	33.0	39.0
12	37.04156269007719	39.0	37.0	39.0	33.0	39.0
13	37.117342054677216	39.0	37.0	39.0	33.0	39.0
14	38.26572181639153	40.0	38.0	41.0	34.0	41.0
15	38.21721775328441	40.0	38.0	41.0	33.0	41.0
16	38.19409153475862	40.0	38.0	41.0	33.0	41.0
17	38.182686261744685	40.0	38.0	41.0	33.0	41.0
18	38.172836253232646	40.0	38.0	41.0	33.0	41.0
19	38.208777577500165	40.0	38.0	41.0	34.0	41.0
20	38.18170245856692	40.0	38.0	41.0	33.0	41.0
21	38.10187837968472	40.0	37.0	41.0	33.0	41.0
22	38.03219431738175	40.0	37.0	41.0	33.0	41.0
23	38.00208480725585	40.0	37.0	41.0	33.0	41.0
24	37.94101201694195	40.0	37.0	41.0	33.0	41.0
25	37.89573739470098	40.0	37.0	41.0	33.0	41.0
26	37.65692901132913	40.0	37.0	41.0	32.0	41.0
27	37.5105006018309	39.0	37.0	41.0	32.0	41.0
28	37.400520816847504	39.0	36.0	41.0	32.0	41.0
29	37.266598684441284	39.0	36.0	41.0	31.0	41.0
30	37.17304670156459	39.0	36.0	41.0	31.0	41.0
31	37.099024494988164	39.0	36.0	40.0	31.0	41.0
32	36.99145631101184	39.0	36.0	40.0	31.0	41.0
33	36.92361666579693	39.0	35.0	40.0	31.0	41.0
34	36.822627130896294	39.0	35.0	40.0	31.0	41.0
35	36.754044072671405	39.0	35.0	40.0	30.0	41.0
36	36.707642781921635	39.0	35.0	40.0	30.0	41.0
37	36.481280791970114	39.0	35.0	40.0	30.0	41.0
38	36.34031890621618	39.0	35.0	40.0	30.0	41.0
39	36.24942746932459	39.0	35.0	40.0	30.0	41.0
40	36.10788642291731	39.0	35.0	40.0	29.0	41.0
41	35.949132242823154	38.0	35.0	40.0	29.0	41.0
42	35.81962353702051	38.0	35.0	40.0	28.0	41.0
43	34.95521471290485	38.0	34.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	9.0
11	5.0
12	0.0
13	2.0
14	5.0
15	4.0
16	14.0
17	35.0
18	62.0
19	121.0
20	316.0
21	589.0
22	963.0
23	1816.0
24	2767.0
25	4298.0
26	6625.0
27	9431.0
28	13041.0
29	17767.0
30	23111.0
31	29631.0
32	37541.0
33	47978.0
34	61908.0
35	81674.0
36	114148.0
37	174978.0
38	297650.0
39	242440.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.12941802481408	20.44146242770116	12.671727122084842	23.757392425399914
2	18.846246961973012	22.02923520851922	34.18938467816376	24.935133151344
3	21.03234317107995	24.608168303914766	29.299540692238136	25.05994783276715
4	14.611701440544497	16.16123421958316	34.76717656187309	34.459887777999256
5	14.026124679515423	37.915090086429245	33.87054690046393	14.188238333591405
6	33.93385249625086	35.07360986472279	15.875332461313011	15.11720517771335
7	28.05866546671195	31.384690140495646	22.121712707229584	18.434931685562816
8	27.79226867579237	32.59536688586942	20.464560415353148	19.147804022985067
9	26.868263621610478	13.897631429688442	21.002230239029952	38.231874709671125
10	17.691604223680912	27.25759303570008	32.51580715062369	22.534995589995322
11	34.521140219328224	22.525414202524864	21.725881637356462	21.227563940790446
12	21.755395732689557	26.78459757744884	29.064454506802356	22.39555218305925
13	29.661751357862254	20.501431647493913	26.10303584551039	23.73378114913344
14	22.55073644083964	21.85437488718344	26.473715773273575	29.121172898703346
15	25.41086614887252	28.12582072710754	23.637197341507168	22.826115782512773
16	24.262040681544622	26.826259503324827	25.618491393433157	23.293208421697393
17	22.71250790250596	27.10446193237765	26.65644652003152	23.526583645084877
18	22.57161017782884	25.389906863780904	28.613359362769298	23.425123595620963
19	23.29295177739015	25.99045454273256	28.58547068138208	22.131122998495208
20	23.963648900321918	25.041726086952803	28.254485073139353	22.74013993958593
21	24.32748497989192	25.56519492562876	27.740854266241094	22.36646582823823
22	23.99341963996226	25.66545730165886	27.944202105680993	22.396920952697887
23	23.276184349316857	25.64552459379622	27.890392349262104	23.187898707624814
24	22.969494402159917	26.3376942904341	27.978335798544485	22.7144755088615
25	23.239313117176092	26.05110814734463	27.78516818329194	22.924410552187336
26	22.982326617522133	26.66962092780339	27.780463037659132	22.567589417015345
27	23.294149450823955	25.92980093812049	27.750264557506714	23.025785053548834
28	22.631579397621593	26.258134555188363	28.2576503529287	22.852635694261348
29	22.51309527577714	26.733610908409634	28.37493680133934	22.378357014473885
30	22.615496354367615	26.807610016998407	28.019826628215643	22.55706700041833
31	22.60069653264986	27.076316606683186	27.731443974975466	22.59154288569148
32	22.292038979137384	26.560718193429395	27.710228045576606	23.437014781856615
33	21.823320926006883	26.683137527984925	28.418822977878115	23.07471856813008
34	23.10919445340323	26.15316703352545	27.90998286471509	22.827655648356235
35	22.616694027801422	26.24016945368126	28.234723461481536	22.908413057035776
36	22.155418659581002	26.90624697908263	27.794236282147907	23.144098079188456
37	22.60531613018026	25.803788583263543	28.390592104081243	23.200303182474958
38	22.27099414594335	25.870516103147057	28.4946185966176	23.36387115429199
39	22.31257052371693	25.838178920434274	28.63269323391503	23.216557321933763
40	21.761982936575492	25.773076814496637	29.15496439915718	23.30997584977069
41	21.14466782955054	25.989684609810826	29.421275641974347	23.444371918664288
42	21.58695151903488	26.026983582463664	29.411779802606308	22.974285095895144
43	21.426805471314438	25.45663438366442	29.173528337381182	23.943031807639958
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	626.0
1	522.0
2	418.0
3	849.0
4	1280.0
5	1280.0
6	1743.5
7	2207.0
8	2129.5
9	2052.0
10	3028.0
11	4004.0
12	4004.0
13	6828.0
14	9652.0
15	12805.0
16	15958.0
17	15520.0
18	15082.0
19	15082.0
20	17594.5
21	20107.0
22	18609.0
23	17111.0
24	19042.5
25	20974.0
26	20974.0
27	23296.5
28	25619.0
29	28534.5
30	31450.0
31	34962.0
32	38474.0
33	38474.0
34	42907.5
35	47341.0
36	51417.0
37	55493.0
38	59619.0
39	63745.0
40	63745.0
41	65415.5
42	67086.0
43	68284.0
44	69482.0
45	70528.5
46	71575.0
47	71575.0
48	73793.0
49	76011.0
50	74710.5
51	73410.0
52	73023.5
53	72637.0
54	72637.0
55	70238.0
56	67839.0
57	65680.5
58	63522.0
59	61886.0
60	60250.0
61	60250.0
62	55410.5
63	50571.0
64	45417.5
65	40264.0
66	34861.5
67	29459.0
68	29459.0
69	25641.5
70	21824.0
71	18417.5
72	15011.0
73	11593.0
74	8175.0
75	8175.0
76	5952.5
77	3730.0
78	3072.0
79	2414.0
80	2009.0
81	1604.0
82	1604.0
83	1279.0
84	954.0
85	814.0
86	674.0
87	589.0
88	504.0
89	504.0
90	401.0
91	298.0
92	164.0
93	30.0
94	20.5
95	11.0
96	11.0
97	7.0
98	3.0
99	2.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1168933.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.23692800317
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.33759555928661	57.38772693918213
2	6.684479586777302	8.587808679089461
3	1.5304351433552634	2.949313563516996
4	0.6304982836599857	1.6200509141435504
5	0.3550199635403324	1.1402695918814183
6	0.23687913037574304	0.9129832586040075
7	0.1581695544366014	0.711222839644621
8	0.12204103632602145	0.6271633011125514
9	0.09240570738306338	0.5342272895023052
>10	0.673326867885745	8.821617086830498
>50	0.09721006226372629	4.4377121676771205
>100	0.07860282387916874	9.25152808355707
>500	0.0025355734311052758	1.0549315128198768
>1k	4.003536996482015E-4	0.483231670548159
>5k	4.003536996482015E-4	1.4802131018902007
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6459	0.5525551934969755	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5733	0.4904472711438551	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5075	0.4341566197549389	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3528	0.30181370531929547	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	8.554810241476629E-5	0.0
2	0.0	0.0	0.0	4.2774051207383144E-4	0.0
3	8.554810241476629E-5	0.0	0.0	9.410291265624292E-4	0.0
4	8.554810241476629E-5	0.0	0.0	0.0013687696386362606	0.0
5	1.7109620482953257E-4	8.554810241476629E-5	0.0	0.0013687696386362606	0.0
6	1.7109620482953257E-4	8.554810241476629E-5	0.0	0.0013687696386362606	0.0
7	1.7109620482953257E-4	8.554810241476629E-5	0.0	0.001796510150710092	0.0
8	1.7109620482953257E-4	8.554810241476629E-5	0.0	0.0019676063555396244	0.0
9	1.7109620482953257E-4	8.554810241476629E-5	0.0	0.0029086354821020537	0.0
10	2.5664430724429884E-4	8.554810241476629E-5	0.0	0.004277405120738314	0.0
11	2.5664430724429884E-4	8.554810241476629E-5	0.0	0.005646174759374575	0.0
12	2.5664430724429884E-4	8.554810241476629E-5	0.0	0.00667275198835177	0.0
13	2.5664430724429884E-4	8.554810241476629E-5	0.0	0.0072715887052551345	0.0
14	2.5664430724429884E-4	8.554810241476629E-5	0.0	0.008383714036647096	0.0
15	2.5664430724429884E-4	8.554810241476629E-5	0.0	0.010864609006675319	0.0
16	2.5664430724429884E-4	8.554810241476629E-5	0.0	0.014714273615339802	0.0
17	2.5664430724429884E-4	8.554810241476629E-5	0.0	0.018478390121589517	0.0
18	3.4219240965906514E-4	8.554810241476629E-5	0.0	0.021044833194032508	0.0
19	3.4219240965906514E-4	8.554810241476629E-5	0.0	0.02326908385681643	0.0
20	3.4219240965906514E-4	8.554810241476629E-5	0.0	0.02626326744133325	0.0
21	3.4219240965906514E-4	8.554810241476629E-5	0.0	0.03139615358621923	0.0
22	3.4219240965906514E-4	8.554810241476629E-5	0.0	0.041319733466332116	0.0
23	3.4219240965906514E-4	8.554810241476629E-5	0.0	0.055777362774427616	0.0
24	3.4219240965906514E-4	8.554810241476629E-5	0.0	0.07861870611917021	0.0
25	3.4219240965906514E-4	8.554810241476629E-5	0.0	0.08494926569786292	0.0
26	3.4219240965906514E-4	8.554810241476629E-5	0.0	0.09735374054800404	0.0
27	4.2774051207383144E-4	8.554810241476629E-5	0.0	0.11685870789857075	0.0
28	4.2774051207383144E-4	1.7109620482953257E-4	0.0	0.15586864259970418	0.0
29	4.2774051207383144E-4	1.7109620482953257E-4	0.0	0.21361361172967142	0.0
30	4.2774051207383144E-4	1.7109620482953257E-4	0.0	0.29445656851162555	0.0
31	4.2774051207383144E-4	1.7109620482953257E-4	0.0	0.5488766250931405	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGACAG	125	1.8189894E-11	20.72	5
CTAGACA	160	3.45608E-11	17.34375	4
GGTATCA	3190	0.0	17.340124	1
TCTGTCG	115	1.2421624E-6	16.086956	8
AAGACGG	275	0.0	15.472728	5
CGAACGA	120	1.9354702E-6	15.416667	16
CGCAAGA	265	0.0	15.358491	2
AAGACCG	85	5.3642463E-4	15.235294	5
ACGGACC	255	0.0	15.235294	8
CGCAATA	135	3.972018E-7	15.074075	36
CGAGTTC	75	0.004104461	14.8	14
TGTCGCC	150	8.100142E-8	14.8	10
ATCTGCG	175	2.237357E-9	14.8	36
CGGAACC	205	9.276846E-11	14.439024	14
TTTGCCG	90	8.274165E-4	14.388888	9
CATACTC	260	0.0	14.230769	3
ATAACGA	130	4.4438293E-6	14.230769	12
ACGAACG	130	4.4438293E-6	14.230769	15
CCCTAGA	105	1.6556689E-4	14.095238	1
ATTCCGT	210	1.364242E-10	14.095238	29
>>END_MODULE