FastQCFastQC Report
Fri 10 Feb 2017
ERR1630611.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630611.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences512482
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT109872.143880175303718No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT84241.643765049309049No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT64611.2607272060286996No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46400.9053976529907393No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGT18970.37015934218177415No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT16440.320791754637236TruSeq Adapter, Index 10 (95% over 21bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT12890.25152102903126355No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12820.25015512739959644No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11160.2177637458486347No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT9650.1882992963655309No Hit
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC8420.16429845340909535No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG8110.1582494604688555No Hit
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCC7900.1541517555738543No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7840.15298098274671112No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATG7600.14829789143813832No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA6810.13288271588075287No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG6420.1252726925043221No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6230.12156524521836866No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA6150.12000421478217771No Hit
GAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTG5700.11122341857860372No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG5650.1102477745559844No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC5540.10810135770622188No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA5510.10751597129265028No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT5410.10556468324741163No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC5390.10517442563836388No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG5370.10478416802931616No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTAGT250.00549353729.61
CCGTCTT2650.027.92452837
GCCGTCT2800.026.4285736
TAACTTT400.001929734623.12500236
GGTCTAG502.6992717E-422.21
TAACTAG450.003822959320.55555526
CCTTATG1052.2520908E-819.380953
CAGTCGG3750.018.74666610
TGTTTAC902.1474843E-618.514
AAATAAC500.00702969318.533
GGACTGA500.00702969318.56
CGTCTTC902.1474843E-618.537
TTTGCAT609.2272984E-418.531
GCAGTCG4000.018.03759
TGCCGTC4000.018.037535
TATACAC3800.018.0131593
TATGCCG3950.017.79746833
TCTTATA6400.017.63281237
ATTAATA953.598503E-617.52631636
CTTATGA953.598503E-617.5263164
Produced by FastQC (version 0.11.5)