##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630611.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	512482
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.84836345471646	33.0	31.0	34.0	30.0	34.0
2	31.973860545345982	33.0	31.0	34.0	30.0	34.0
3	31.995726679180926	33.0	31.0	34.0	30.0	34.0
4	35.646204159365595	37.0	35.0	37.0	33.0	37.0
5	35.67338755312382	37.0	35.0	37.0	33.0	37.0
6	35.75500602948006	37.0	35.0	37.0	35.0	37.0
7	35.71240550887641	37.0	35.0	37.0	33.0	37.0
8	35.746467192994096	37.0	35.0	37.0	33.0	37.0
9	37.3746160840771	39.0	37.0	39.0	34.0	39.0
10	37.28789303819451	39.0	37.0	39.0	34.0	39.0
11	37.44859721902428	39.0	37.0	39.0	35.0	39.0
12	37.388690724747406	39.0	37.0	39.0	34.0	39.0
13	37.45001580543317	39.0	37.0	39.0	35.0	39.0
14	38.56537790595572	40.0	38.0	41.0	34.0	41.0
15	38.525390160044644	40.0	38.0	41.0	34.0	41.0
16	38.426695962004516	40.0	38.0	41.0	34.0	41.0
17	38.511957493141225	40.0	38.0	41.0	34.0	41.0
18	38.56047627038608	40.0	38.0	41.0	34.0	41.0
19	38.63274807700563	40.0	38.0	41.0	34.0	41.0
20	38.62609028219528	40.0	38.0	41.0	34.0	41.0
21	38.55543414207718	40.0	38.0	41.0	34.0	41.0
22	38.45829707189716	40.0	38.0	41.0	34.0	41.0
23	38.40733918459575	40.0	38.0	41.0	34.0	41.0
24	38.333256582670224	40.0	38.0	41.0	34.0	41.0
25	38.24805163888683	40.0	37.0	41.0	34.0	41.0
26	37.96751690791091	40.0	37.0	41.0	33.0	41.0
27	37.759704731092995	40.0	37.0	41.0	33.0	41.0
28	37.63012554587283	40.0	36.0	41.0	33.0	41.0
29	37.46211769388974	39.0	36.0	41.0	32.0	41.0
30	37.27319788792582	39.0	35.0	41.0	32.0	41.0
31	37.077893467477885	39.0	35.0	41.0	32.0	41.0
32	36.846316553557	39.0	35.0	41.0	31.0	41.0
33	36.66691122810167	39.0	35.0	41.0	31.0	41.0
34	36.49264364406945	39.0	35.0	41.0	31.0	41.0
35	36.30784300716903	39.0	35.0	41.0	30.0	41.0
36	36.14061176782794	39.0	35.0	41.0	30.0	41.0
37	35.801548151935094	38.0	35.0	40.0	29.0	41.0
38	35.54135950140688	38.0	35.0	40.0	27.0	41.0
39	35.29189317868725	38.0	35.0	40.0	25.0	41.0
40	34.97742749989268	38.0	35.0	40.0	24.0	41.0
41	34.67729403179038	38.0	34.0	40.0	22.0	41.0
42	34.36396010006205	38.0	34.0	40.0	21.0	41.0
43	33.42139040980952	37.0	33.0	40.0	17.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	4.0
11	3.0
12	4.0
13	2.0
14	1.0
15	1.0
16	4.0
17	13.0
18	25.0
19	72.0
20	143.0
21	272.0
22	506.0
23	854.0
24	1323.0
25	2167.0
26	3178.0
27	4674.0
28	6453.0
29	8583.0
30	10899.0
31	13653.0
32	16759.0
33	20949.0
34	26884.0
35	34209.0
36	47089.0
37	77317.0
38	107969.0
39	128469.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.80141351305997	20.32227473355162	12.993236835635201	20.88307491775321
2	19.569467805698544	22.2442934581117	33.44390632256353	24.742332413626233
3	22.816411112975675	25.357963791899035	29.613332760955508	22.212292334169785
4	13.829558891824492	16.889568804367762	32.49148262768253	36.78938967612521
5	12.598686392887945	40.48356820337104	32.160934432819104	14.756810970921904
6	31.995270077778343	32.53870379837731	16.803712130377264	18.662313993467087
7	26.8089415823385	33.684109880932404	20.667847846363387	18.839100690365708
8	30.568098001490785	30.563414910182214	19.618250006829506	19.250237081497495
9	28.071229818803396	11.816219886747243	22.09053196014689	38.02201833430247
10	20.928344800402744	28.208795625992718	29.283174823701124	21.57968474990341
11	32.10063963222123	22.743823197692798	19.68498405797667	25.4705531121093
12	22.020090461713774	30.608879921636273	26.47722261464793	20.89380700200202
13	30.6900535043182	19.56439445678092	27.981665697526935	21.763886341373944
14	23.24159677803318	22.733676499857555	28.206649209142952	25.81807751296631
15	28.395924149531105	24.9848775176494	26.27702046120644	20.34217787161305
16	23.926889139521	24.744869088085046	28.85857454505719	22.469667227336764
17	21.549439785202214	28.18303862379557	27.77307300549092	22.494448585511297
18	20.600528408802653	23.1305684882591	31.789214060201136	24.47968904273711
19	21.60270994883723	25.18605531511351	32.66885471099473	20.542380025054538
20	22.6956263829754	22.452105634929616	34.32062004128926	20.531647940805726
21	23.557705441361843	23.946987406386956	31.97087897721286	20.52442817503834
22	24.22075311913394	24.422906560620667	31.222755140668358	20.133585179577036
23	22.251708352683604	24.177629653334165	32.06668722023407	21.50397477374815
24	21.37967772526645	25.968716950058734	31.323246474998147	21.328358849676672
25	21.10103379240637	25.432307866422626	32.384747171607984	21.08191116956303
26	21.811107512068716	26.136722850753785	31.50100881591939	20.55116082125811
27	21.42728915357027	26.315655964502167	31.3310516271791	20.92600325474846
28	20.24578424217826	25.77163685748963	32.763297052384274	21.21928184794783
29	20.32637243844662	26.261605285649058	32.61460890333709	20.79741337256723
30	19.464683637669225	27.09695169781573	33.57191081833118	19.866453846183866
31	20.561892905506927	28.014837594295994	30.9230372969197	20.50023220327738
32	19.4473171740666	27.346131181192707	32.26376731280318	20.94278433193751
33	19.44614640123946	26.861040973146373	32.72797873876546	20.96483388684871
34	20.083827334423454	27.06046261136977	32.28386557966914	20.57184447453764
35	19.278530758153455	27.85619787621809	32.44191991133347	20.42335145429498
36	19.92518761634555	28.58988218122783	30.385847698065493	21.099082504361128
37	19.500587337701617	27.735608275022344	31.422176778891746	21.341627608384293
38	19.69649665744358	26.96328846671688	31.496130595806292	21.84408428003325
39	19.690642793307862	27.879027946347385	31.3400275521872	21.090301708157554
40	18.57372551621325	27.894833379513816	31.721504365031354	21.80993673924157
41	18.97237366385551	27.581456519448487	31.069579029117122	22.37659078757888
42	18.622117459735172	28.346361433182043	31.76462783083113	21.266893276251654
43	18.554602893369914	27.188271978332896	31.283049941266228	22.97407518703096
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	136.0
1	148.5
2	161.0
3	616.5
4	1072.0
5	1072.0
6	1624.5
7	2177.0
8	2270.0
9	2363.0
10	3270.0
11	4177.0
12	4177.0
13	7748.5
14	11320.0
15	15379.0
16	19438.0
17	17328.0
18	15218.0
19	15218.0
20	17298.0
21	19378.0
22	13584.5
23	7791.0
24	7044.0
25	6297.0
26	6297.0
27	6712.5
28	7128.0
29	7931.0
30	8734.0
31	9934.5
32	11135.0
33	11135.0
34	13343.0
35	15551.0
36	17123.0
37	18695.0
38	22048.0
39	25401.0
40	25401.0
41	26854.0
42	28307.0
43	30414.0
44	32521.0
45	32042.5
46	31564.0
47	31564.0
48	33920.0
49	36276.0
50	35484.5
51	34693.0
52	32762.5
53	30832.0
54	30832.0
55	30642.0
56	30452.0
57	27076.0
58	23700.0
59	23472.5
60	23245.0
61	23245.0
62	21132.0
63	19019.0
64	16264.5
65	13510.0
66	12309.5
67	11109.0
68	11109.0
69	9195.5
70	7282.0
71	6485.5
72	5689.0
73	4465.0
74	3241.0
75	3241.0
76	2587.0
77	1933.0
78	1487.5
79	1042.0
80	817.0
81	592.0
82	592.0
83	544.5
84	497.0
85	454.0
86	411.0
87	307.0
88	203.0
89	203.0
90	179.0
91	155.0
92	93.0
93	31.0
94	17.5
95	4.0
96	4.0
97	3.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	512482.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.45362519399273
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.53363503343412	33.798647473797736
2	9.361695969093411	7.761524717658215
3	2.9024413932050885	3.6095015298436146
4	1.398257204861331	2.318513203804861
5	0.8581314531514204	1.7786329813057655
6	0.5781371844204652	1.4379529292205728
7	0.4479721900761747	1.2999049885324871
8	0.334985104230275	1.1109077565065926
9	0.2798358971511985	1.0440191156697431
>10	1.901357307335067	15.6368240271515
>50	0.21194720497373012	6.199411018978415
>100	0.1793035506362193	14.372921918282126
>500	0.008042639474447832	2.22983465520778
>1k	0.0028385786380404118	2.327500236412131
>5k	9.461928793468039E-4	2.9191810767605806
>10k+	4.7309643967340196E-4	2.154722370867887
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	10987	2.143880175303718	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	8424	1.643765049309049	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6461	1.2607272060286996	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4640	0.9053976529907393	No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGT	1897	0.37015934218177415	No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT	1644	0.320791754637236	TruSeq Adapter, Index 10 (95% over 21bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1289	0.25152102903126355	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1282	0.25015512739959644	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1116	0.2177637458486347	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	965	0.1882992963655309	No Hit
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC	842	0.16429845340909535	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	811	0.1582494604688555	No Hit
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCC	790	0.1541517555738543	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	784	0.15298098274671112	No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATG	760	0.14829789143813832	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	681	0.13288271588075287	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	642	0.1252726925043221	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	623	0.12156524521836866	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	615	0.12000421478217771	No Hit
GAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTG	570	0.11122341857860372	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	565	0.1102477745559844	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	554	0.10810135770622188	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	551	0.10751597129265028	No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT	541	0.10556468324741163	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	539	0.10517442563836388	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	537	0.10478416802931616	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.951288045238662E-4	0.0	5.853864135715986E-4	0.0
3	0.0	1.951288045238662E-4	0.0	9.75644022619331E-4	0.0
4	0.0	1.951288045238662E-4	0.0	0.0013659016316670634	0.0
5	0.0	3.902576090477324E-4	0.0	0.0015610304361909297	0.0
6	0.0	3.902576090477324E-4	0.0	0.0015610304361909297	0.0
7	0.0	3.902576090477324E-4	0.0	0.001756159240714796	0.0
8	0.0	3.902576090477324E-4	0.0	0.001756159240714796	0.0
9	0.0	3.902576090477324E-4	0.0	0.0027318032633341267	0.0
10	0.0	3.902576090477324E-4	0.0	0.003902576090477324	0.0
11	0.0	3.902576090477324E-4	0.0	0.004683091308572789	0.0
12	0.0	3.902576090477324E-4	0.0	0.006439250549287585	0.0
13	0.0	3.902576090477324E-4	0.0	0.007024636962859184	0.0
14	0.0	3.902576090477324E-4	0.0	0.008000280985478515	0.0
15	0.0	3.902576090477324E-4	0.0	0.010146697835241043	0.0
16	0.0	3.902576090477324E-4	0.0	0.020488524475005952	0.0
17	0.0	5.853864135715986E-4	0.0	0.033757283182628856	0.0
18	0.0	5.853864135715986E-4	0.0	0.03726960166405845	0.0
19	1.951288045238662E-4	5.853864135715986E-4	0.0	0.046050397867632424	0.0
20	1.951288045238662E-4	5.853864135715986E-4	0.0	0.05131887558977681	0.0
21	1.951288045238662E-4	5.853864135715986E-4	0.0	0.06731943756073384	0.0
22	1.951288045238662E-4	5.853864135715986E-4	0.0	0.09444234138955124	0.0
23	1.951288045238662E-4	5.853864135715986E-4	0.0	0.12722398054956077	0.0
24	1.951288045238662E-4	5.853864135715986E-4	0.0	0.18166491701171944	0.0
25	1.951288045238662E-4	5.853864135715986E-4	0.0	0.20000702463696285	0.0
26	1.951288045238662E-4	5.853864135715986E-4	0.0	0.22030042030744496	0.0
27	1.951288045238662E-4	5.853864135715986E-4	0.0	0.24566716489554755	0.0
28	1.951288045238662E-4	5.853864135715986E-4	0.0	0.2841075393867492	0.0
29	1.951288045238662E-4	5.853864135715986E-4	0.0	0.34264618074390907	0.0
30	1.951288045238662E-4	5.853864135715986E-4	0.0	0.44957676562298776	0.0
31	1.951288045238662E-4	5.853864135715986E-4	0.0	0.7370014946866427	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCTAGT	25	0.005493537	29.6	1
CCGTCTT	265	0.0	27.924528	37
GCCGTCT	280	0.0	26.42857	36
TAACTTT	40	0.0019297346	23.125002	36
GGTCTAG	50	2.6992717E-4	22.2	1
TAACTAG	45	0.0038229593	20.555555	26
CCTTATG	105	2.2520908E-8	19.38095	3
CAGTCGG	375	0.0	18.746666	10
TGTTTAC	90	2.1474843E-6	18.5	14
AAATAAC	50	0.007029693	18.5	33
GGACTGA	50	0.007029693	18.5	6
CGTCTTC	90	2.1474843E-6	18.5	37
TTTGCAT	60	9.2272984E-4	18.5	31
GCAGTCG	400	0.0	18.0375	9
TGCCGTC	400	0.0	18.0375	35
TATACAC	380	0.0	18.013159	3
TATGCCG	395	0.0	17.797468	33
TCTTATA	640	0.0	17.632812	37
ATTAATA	95	3.598503E-6	17.526316	36
CTTATGA	95	3.598503E-6	17.526316	4
>>END_MODULE