FastQCFastQC Report
Fri 10 Feb 2017
ERR1630610.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630610.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1580354
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT97840.6191017961798433No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT81300.5144417010366031No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT74640.4722992443465198No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60750.38440754413251715No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18480.11693582577068176No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT16560.1047866490672343No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16060.1016228009673782No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT701.2195647E-418.56
ACGGGTA500.00703602318.4999985
AAGACGG2400.016.18755
GGTATCA52850.015.54211
TAGTACT1508.106326E-814.84
CAAGACG2900.014.6724144
GACGGAC2800.014.5357147
ACGGACC2850.014.2807028
TCTAGCG1702.4381734E-814.14705928
CAGTCGG2700.013.70370310
AGCGTCA1102.457274E-413.4545453
CTAGCGG1652.5964619E-713.45454529
ACGTTAA1409.563457E-613.21428520
CTATACT2800.013.2142854
GCGCAAG3100.013.1290321
CGAACGT850.00940676813.0588244
TACTAGG850.00940676813.0588242
CGCAATA1857.386916E-813.00000136
AGCACCG1153.5784885E-412.8695655
GTCTAGT1153.5784885E-412.8695651
Produced by FastQC (version 0.11.5)