FastQCFastQC Report
Fri 10 Feb 2017
ERR1630609.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630609.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences658917
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT33060.5017323881460032No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT26450.401416263353351No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT23290.3534587816067881No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA20370.3091436402460401No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGT17630.2675602541746533No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT17020.2583026390273737RNA PCR Primer, Index 39 (95% over 23bp)
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC16740.25405324191058964No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG16540.2510179582557439No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC16120.2446438625805678No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT15940.24191210729120663No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC14220.21580866785953315No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13130.19926637194062377No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA12820.19456168227561288No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTC12540.1903122851588288No Hit
GAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTG12410.18833935078317907No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC12000.18211701929074525No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC11620.17634998034653834No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC11240.1705829414023314No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCAT11220.17027941303684682No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC11010.1670923651992588No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTG10910.1655747233718359No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC10630.16132532625505186No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC10590.1607182695240827No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG10440.15844180678294836No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG10150.15404064548342206No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGA10100.1532818245697106No Hit
GATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTT10050.15252300365599916No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG9780.14842537072195738No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG9490.14402420942243105No Hit
CGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA9310.14129245413306987No Hit
GTCCTAAACTACTAAACCTGCATTAAAAATTTCGGTTGGGGCG9260.14053363321935844No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC9220.13992657648838927No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAG9210.13977481230564698No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATG9190.13947128394016242No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC9110.1382571704782241No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC8810.13370424499595548No Hit
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC8610.13066896134110972RNA PCR Primer, Index 39 (95% over 21bp)
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT8470.12854426278271772No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT8400.1274819135035217No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG8400.1274819135035217No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGAT8370.12702662095529482No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT8330.12641956422432568No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT8190.12429486566593365No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC8170.12399133730044906No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC8150.12368780893496449No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGAC8140.1235360447522222No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG8070.1224736954730262No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC7870.11943841181818043No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG7820.118679590904469No Hit
GTCCTACGTGATCTGAGTTCAGACCGGAGTAATCCAGGTCGGT7570.11488548633591181No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG7540.11443019378768494No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG7540.11443019378768494No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAAT7530.11427842960494265No Hit
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCC7530.11427842960494265No Hit
GGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAAC7510.11397490123945808No Hit
GTTTACGACCTCGATGTTGGATCAGGACATCCCGATGGTGCAG7220.10957373993993173No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATG7090.107600805564282No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA7020.10653845628508599No Hit
GTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTG6940.10532434282314769No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA6930.1051725786404054No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCT6890.10456552190943624No Hit
CTACTAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCGGA6870.10426199354395167No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT6830.10365493681298252No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA6770.10274435171652879No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT6750.10244082335104422No Hit
GTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACAC6740.10228905916830193No Hit
GTACATGGGCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCAC6700.10168200243733276No Hit
GCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGAT6680.1013784740718482No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCTCG250.005494661629.616
AATGGAG250.005494661629.65
GTACAAG405.936343E-527.751
CCGTCTT1900.027.26315937
GCTAAAG358.865363E-426.4285729
TAAGATA358.865363E-426.428574
CGTCTTC509.0834E-625.90000237
GCCGTCT2100.024.66666636
TTGGCGC400.001930317723.1255
AGTGTTC400.001930317723.1258
TAAAGTA652.6799607E-622.7692326
AGTTCCT1155.456968E-1222.5217416
ATAAAGA502.7004062E-422.217
TATTAGA2700.021.9259262
TTAGCCA1400.021.1428574
CCCAAAT705.096472E-621.14285720
GGTATCA16800.021.1428571
GCCAAAC705.096472E-621.1428577
GCCGAGT1251.8189894E-1120.7212
GAGTCCG450.003824101320.55555521
Produced by FastQC (version 0.11.5)