##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630609.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	658917
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.89447836373929	33.0	31.0	34.0	30.0	34.0
2	32.07169188228563	33.0	31.0	34.0	30.0	34.0
3	32.150870291705935	34.0	31.0	34.0	30.0	34.0
4	35.73792905631514	37.0	35.0	37.0	33.0	37.0
5	35.740780705308865	37.0	35.0	37.0	35.0	37.0
6	35.79105107320042	37.0	35.0	37.0	35.0	37.0
7	35.77823003504235	37.0	35.0	37.0	35.0	37.0
8	35.768297069281864	37.0	35.0	37.0	35.0	37.0
9	37.46909398300545	39.0	37.0	39.0	35.0	39.0
10	37.35488081199908	39.0	37.0	39.0	34.0	39.0
11	37.47751841278947	39.0	37.0	39.0	35.0	39.0
12	37.413956537773345	39.0	37.0	39.0	34.0	39.0
13	37.47020034389764	39.0	37.0	39.0	35.0	39.0
14	38.67070359392761	40.0	38.0	41.0	35.0	41.0
15	38.63954944249427	40.0	38.0	41.0	34.0	41.0
16	38.59632396796562	40.0	38.0	41.0	34.0	41.0
17	38.601678208332764	40.0	38.0	41.0	34.0	41.0
18	38.58850659491256	40.0	38.0	41.0	34.0	41.0
19	38.62999133426516	40.0	38.0	41.0	34.0	41.0
20	38.590370259076636	40.0	38.0	41.0	34.0	41.0
21	38.5118322945075	40.0	38.0	41.0	34.0	41.0
22	38.45502392562341	40.0	38.0	41.0	34.0	41.0
23	38.42111828955696	40.0	38.0	41.0	34.0	41.0
24	38.371609777862766	40.0	38.0	41.0	34.0	41.0
25	38.297752220689404	40.0	38.0	41.0	34.0	41.0
26	38.09056527605146	40.0	37.0	41.0	33.0	41.0
27	37.94915596349768	40.0	37.0	41.0	33.0	41.0
28	37.82797226357796	40.0	37.0	41.0	33.0	41.0
29	37.738566465882656	40.0	37.0	41.0	32.0	41.0
30	37.61581504195521	40.0	37.0	41.0	32.0	41.0
31	37.602107700969924	40.0	37.0	41.0	32.0	41.0
32	37.48610067732355	40.0	37.0	41.0	32.0	41.0
33	37.43004961171134	40.0	36.0	41.0	32.0	41.0
34	37.34547598559455	40.0	36.0	41.0	31.0	41.0
35	37.22688138263241	40.0	36.0	41.0	31.0	41.0
36	37.20420933137254	39.0	36.0	41.0	31.0	41.0
37	36.99848691109806	39.0	35.0	41.0	31.0	41.0
38	36.86318914218331	39.0	35.0	41.0	30.0	41.0
39	36.76990576961894	39.0	35.0	41.0	30.0	41.0
40	36.63457613022581	39.0	35.0	41.0	30.0	41.0
41	36.51432426238813	39.0	35.0	41.0	30.0	41.0
42	36.357304485997474	39.0	35.0	41.0	30.0	41.0
43	35.4410828677967	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	1.0
10	2.0
11	0.0
12	1.0
13	2.0
14	2.0
15	1.0
16	3.0
17	11.0
18	24.0
19	47.0
20	112.0
21	234.0
22	447.0
23	769.0
24	1248.0
25	1985.0
26	3016.0
27	4409.0
28	6041.0
29	8355.0
30	10899.0
31	14276.0
32	18028.0
33	23074.0
34	30279.0
35	39871.0
36	55159.0
37	85394.0
38	151373.0
39	203851.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.08554795217	19.882018524336146	12.159346321312093	24.87308720218176
2	21.336981744286458	19.53736206532841	33.396315469171384	25.72934072121375
3	21.243039715168983	23.48307905244515	29.01640115522896	26.25748007715691
4	13.962153048107728	15.808819623715886	34.9948476059959	35.234179722180485
5	14.034696327458542	37.141096678337334	34.230411417522994	14.59379557668113
6	33.56310430600516	34.88770209297984	15.500283040200816	16.048910560814182
7	28.98665537541147	30.174665397918098	21.036033369908502	19.80264585676193
8	28.570366222149374	30.636332041820136	19.045949641608885	21.747352094421604
9	27.36975977247514	12.889028511937012	20.67847695536767	39.06273476022018
10	20.56814439451403	25.171152057087614	31.012403686655528	23.24829986174283
11	35.73424270431633	21.961036063722744	19.898409056072314	22.40631217588862
12	23.75655810974675	26.839951010521812	24.858062548090277	24.545428331641162
13	30.08527629428289	19.237779568595133	25.675919728888463	25.001024408233512
14	23.122032061701244	21.35048875655052	24.321424397913546	31.206054783834684
15	27.76935486563558	26.246553055999467	23.11884501386366	22.865247064501297
16	26.989742258888448	24.635424492007342	23.881460032143654	24.493373216960556
17	25.66772446302038	25.642683372867904	23.70738651453825	24.982205649573466
18	23.961743284814325	22.87951289767907	26.942240069690115	26.216503747816493
19	24.660920874708044	24.160706128389464	26.45932644020415	24.71904655669834
20	24.972644506060703	22.438182654264498	28.139962999892248	24.44920983978255
21	25.07857590561482	24.518566071295776	26.33897744328952	24.063880579799886
22	26.795028812430093	24.26117401736486	25.16083209266114	23.78296507754391
23	24.809498009612742	23.05055113162963	26.802465257384466	25.33748560137316
24	24.052042973545984	25.33217385497718	25.189819051564914	25.425964119911914
25	24.355116046482333	24.16723198824738	26.69926561311971	24.778386352150576
26	24.78946513749076	25.317149200885698	25.67956206927428	24.213823592349264
27	25.583950634146635	24.50156848282864	25.791564036138087	24.122916846886632
28	23.952485669667045	23.870836539351696	27.007043375721068	25.169634415260195
29	24.25874579044098	24.2308211808164	27.439874824902073	24.070558203840545
30	21.854345843254915	25.211369565514318	28.60967314547963	24.324611445751135
31	25.015290241411286	25.281939910489488	25.192550806854282	24.51021904124495
32	23.217643496828888	24.220197688024438	26.47966284069162	26.082495974455057
33	22.455483771097118	24.1432532473741	26.997633996391045	26.403628985137733
34	24.664259686728375	23.512066011348924	27.943276619058246	23.88039768286446
35	23.21506350572227	25.595181183669567	27.376285632333058	23.81346967827511
36	23.977678524002265	25.406690068703647	25.892638981844453	24.72299242544964
37	24.172847263008844	23.5676117022326	27.409218459988132	24.85032257477042
38	23.451360338252012	22.78526734019611	27.4245466424451	26.338825679106776
39	23.626951497684836	24.306247979639316	27.544592110994255	24.522208411681593
40	22.599507980519547	24.209270666866995	27.573275541532542	25.61794581108091
41	23.231909330006662	23.486417864465476	27.585871968700154	25.695800836827704
42	22.61817497499685	24.969457458223115	28.474147730290767	23.938219836489267
43	22.38051226482243	22.2591009186286	28.161817042207137	27.198569774341834
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	20.0
1	31.5
2	43.0
3	165.5
4	288.0
5	288.0
6	456.0
7	624.0
8	660.0
9	696.0
10	942.5
11	1189.0
12	1189.0
13	2188.5
14	3188.0
15	4599.0
16	6010.0
17	5455.0
18	4900.0
19	4900.0
20	5359.5
21	5819.0
22	4183.0
23	2547.0
24	2401.0
25	2255.0
26	2255.0
27	2905.5
28	3556.0
29	4946.5
30	6337.0
31	8621.5
32	10906.0
33	10906.0
34	15383.5
35	19861.0
36	23060.5
37	26260.0
38	32139.0
39	38018.0
40	38018.0
41	40646.0
42	43274.0
43	47280.0
44	51286.0
45	49756.0
46	48226.0
47	48226.0
48	51073.0
49	53920.0
50	54616.0
51	55312.0
52	52859.0
53	50406.0
54	50406.0
55	48554.5
56	46703.0
57	41497.0
58	36291.0
59	34281.0
60	32271.0
61	32271.0
62	30617.0
63	28963.0
64	27031.5
65	25100.0
66	23548.5
67	21997.0
68	21997.0
69	17177.5
70	12358.0
71	10823.5
72	9289.0
73	6862.0
74	4435.0
75	4435.0
76	3457.0
77	2479.0
78	1969.5
79	1460.0
80	1170.0
81	880.0
82	880.0
83	766.0
84	652.0
85	597.5
86	543.0
87	449.5
88	356.0
89	356.0
90	262.0
91	168.0
92	96.0
93	24.0
94	14.0
95	4.0
96	4.0
97	3.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	658917.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.811901108878782
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.57826758989772	19.766306701196974
2	9.772774051241626	5.04507754740131
3	3.333856335620327	2.5815951013872263
4	1.8933634792328857	1.9548524355648764
5	1.2372590905687388	1.5968004645910794
6	0.8609764060953312	1.3334062710726329
7	0.6997798077917737	1.2643853037698025
8	0.5590465650723025	1.1544043722323711
9	0.44144393632884776	1.025505650667096
>10	3.6367965413989283	19.260757739189128
>50	0.5121198698105115	9.146272658185215
>100	0.4105228483486799	21.330884370735646
>500	0.04784510894423464	8.467952181826782
>1k	0.015948369648078213	6.071799202179866
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3306	0.5017323881460032	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2645	0.401416263353351	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2329	0.3534587816067881	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	2037	0.3091436402460401	No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGT	1763	0.2675602541746533	No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT	1702	0.2583026390273737	RNA PCR Primer, Index 39 (95% over 23bp)
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	1674	0.25405324191058964	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	1654	0.2510179582557439	No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC	1612	0.2446438625805678	No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT	1594	0.24191210729120663	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	1422	0.21580866785953315	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1313	0.19926637194062377	No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA	1282	0.19456168227561288	No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTC	1254	0.1903122851588288	No Hit
GAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTG	1241	0.18833935078317907	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	1200	0.18211701929074525	No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC	1162	0.17634998034653834	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	1124	0.1705829414023314	No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCAT	1122	0.17027941303684682	No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC	1101	0.1670923651992588	No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTG	1091	0.1655747233718359	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	1063	0.16132532625505186	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1059	0.1607182695240827	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	1044	0.15844180678294836	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	1015	0.15404064548342206	No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGA	1010	0.1532818245697106	No Hit
GATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTT	1005	0.15252300365599916	No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG	978	0.14842537072195738	No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG	949	0.14402420942243105	No Hit
CGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA	931	0.14129245413306987	No Hit
GTCCTAAACTACTAAACCTGCATTAAAAATTTCGGTTGGGGCG	926	0.14053363321935844	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	922	0.13992657648838927	No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAG	921	0.13977481230564698	No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATG	919	0.13947128394016242	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	911	0.1382571704782241	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	881	0.13370424499595548	No Hit
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC	861	0.13066896134110972	RNA PCR Primer, Index 39 (95% over 21bp)
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT	847	0.12854426278271772	No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT	840	0.1274819135035217	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	840	0.1274819135035217	No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGAT	837	0.12702662095529482	No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT	833	0.12641956422432568	No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT	819	0.12429486566593365	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	817	0.12399133730044906	No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC	815	0.12368780893496449	No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGAC	814	0.1235360447522222	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG	807	0.1224736954730262	No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC	787	0.11943841181818043	No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG	782	0.118679590904469	No Hit
GTCCTACGTGATCTGAGTTCAGACCGGAGTAATCCAGGTCGGT	757	0.11488548633591181	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	754	0.11443019378768494	No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG	754	0.11443019378768494	No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAAT	753	0.11427842960494265	No Hit
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCC	753	0.11427842960494265	No Hit
GGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAAC	751	0.11397490123945808	No Hit
GTTTACGACCTCGATGTTGGATCAGGACATCCCGATGGTGCAG	722	0.10957373993993173	No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATG	709	0.107600805564282	No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA	702	0.10653845628508599	No Hit
GTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTG	694	0.10532434282314769	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	693	0.1051725786404054	No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCT	689	0.10456552190943624	No Hit
CTACTAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCGGA	687	0.10426199354395167	No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT	683	0.10365493681298252	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	677	0.10274435171652879	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	675	0.10244082335104422	No Hit
GTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACAC	674	0.10228905916830193	No Hit
GTACATGGGCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCAC	670	0.10168200243733276	No Hit
GCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGAT	668	0.1013784740718482	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0028835194721034667	0.0
3	0.0	0.0	0.0	0.005463510578722358	0.0
4	0.0	0.0	0.0	0.008195265868083538	0.0
5	0.0	0.0	0.0	0.0086505584163104	0.0
6	0.0	0.0	0.0	0.008954086781794976	0.0
7	0.0	0.0	0.0	0.01001643606099099	0.0
8	0.0	0.0	0.0	0.010471728609217853	0.0
9	0.0	0.0	0.0	0.015783475005197924	0.0
10	0.0	0.0	0.0	0.02139874976666257	0.0
11	0.0	0.0	0.0	0.02397874087328146	0.0
12	0.0	0.0	0.0	0.026406967797158065	0.0
13	0.0	0.0	0.0	0.02746931707635408	0.0
14	0.0	0.0	0.0	0.02913872308651924	0.0
15	0.0	0.0	0.0	0.031870478375880425	0.0
16	0.0	0.0	0.0	0.036575168040891344	0.0
17	0.0	0.0	0.0	0.04067280097493311	0.0
18	0.0	0.0	0.0	0.043101027898809714	0.0
19	0.0	0.0	0.0	0.0494751235739858	0.0
20	0.0	0.0	0.0	0.054331577421739004	0.0
21	0.0	0.0	0.0	0.06252684328982254	0.0
22	0.0	0.0	0.0	0.07406092117823641	0.0
23	0.0	0.0	0.0	0.09181733055908407	0.0
24	0.0	0.0	0.0	0.1160995997978501	0.0
25	0.0	0.0	0.0	0.12414310148319135	0.0
26	0.0	0.0	0.0	0.13795364211273953	0.0
27	0.0	0.0	0.0	0.15783475005197922	0.0
28	0.0	0.0	0.0	0.1868217089557562	0.0
29	0.0	0.0	0.0	0.22977097267182361	0.0
30	0.0	0.0	0.0	0.3115718671699167	0.0
31	0.0	0.0	0.0	0.5449851802275553	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGCTCG	25	0.0054946616	29.6	16
AATGGAG	25	0.0054946616	29.6	5
GTACAAG	40	5.936343E-5	27.75	1
CCGTCTT	190	0.0	27.263159	37
GCTAAAG	35	8.865363E-4	26.42857	29
TAAGATA	35	8.865363E-4	26.42857	4
CGTCTTC	50	9.0834E-6	25.900002	37
GCCGTCT	210	0.0	24.666666	36
TTGGCGC	40	0.0019303177	23.125	5
AGTGTTC	40	0.0019303177	23.125	8
TAAAGTA	65	2.6799607E-6	22.76923	26
AGTTCCT	115	5.456968E-12	22.52174	16
ATAAAGA	50	2.7004062E-4	22.2	17
TATTAGA	270	0.0	21.925926	2
TTAGCCA	140	0.0	21.142857	4
CCCAAAT	70	5.096472E-6	21.142857	20
GGTATCA	1680	0.0	21.142857	1
GCCAAAC	70	5.096472E-6	21.142857	7
GCCGAGT	125	1.8189894E-11	20.72	12
GAGTCCG	45	0.0038241013	20.555555	21
>>END_MODULE