FastQCFastQC Report
Fri 10 Feb 2017
ERR1630608.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630608.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1050650
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT31740.302098700804264No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT29410.27992195307666684No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT23830.22681197354018942No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17300.1646599723980393No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT12200.1161185932517965No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG12020.11440536810545852No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGTA250.00549613129.69
GGTATCA14750.017.9355931
TCTAGCG1156.4033884E-817.69565228
AGCACCG904.445372E-516.4444455
TAGACTA803.3822184E-416.18755
CCGATCT803.3822184E-416.18759
AAGACGG2400.016.18755
TTAGAGT1751.3096724E-1015.8571434
TATACTG1059.341491E-615.8571425
ATTAGAG1902.7284841E-1115.5789483
TATTCCG957.058754E-515.5789485
CTAGACA855.363606E-415.2352944
TGAACCG855.363606E-415.23529414
TCTAGAC855.363606E-415.2352943
TCAGTAG1353.9708902E-715.0740733
AGAGTGT2109.094947E-1214.976196
GAGTGTT1752.235538E-914.87
TAGGGTC1001.0932215E-414.84
ATAACTA2006.184564E-1114.85
AGTACTA750.004104094614.7999995
Produced by FastQC (version 0.11.5)