##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630608.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1050650
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.19211726074335	31.0	31.0	34.0	28.0	34.0
2	31.374284490553467	31.0	31.0	34.0	28.0	34.0
3	31.46427640032361	31.0	31.0	34.0	28.0	34.0
4	35.224469614048445	37.0	35.0	37.0	32.0	37.0
5	34.95103412173417	35.0	35.0	37.0	32.0	37.0
6	35.00239565982963	36.0	35.0	37.0	32.0	37.0
7	34.99830866606386	36.0	35.0	37.0	32.0	37.0
8	34.973421215438066	36.0	35.0	37.0	32.0	37.0
9	36.561963546376056	38.0	35.0	39.0	32.0	39.0
10	36.32567553419312	38.0	35.0	39.0	32.0	39.0
11	36.51543520677676	38.0	35.0	39.0	32.0	39.0
12	36.407079427021365	38.0	35.0	39.0	32.0	39.0
13	36.50918859753486	38.0	35.0	39.0	32.0	39.0
14	37.55021748441441	39.0	37.0	40.0	32.0	41.0
15	37.50611621377243	39.0	36.0	40.0	32.0	41.0
16	37.47027554371103	39.0	36.0	40.0	32.0	41.0
17	37.4147137486318	39.0	36.0	40.0	32.0	41.0
18	37.374206443630136	39.0	36.0	40.0	32.0	41.0
19	37.404515300052346	39.0	36.0	40.0	32.0	41.0
20	37.37923380764289	39.0	36.0	40.0	32.0	41.0
21	37.28700994622377	39.0	36.0	40.0	32.0	41.0
22	37.22734497691905	39.0	36.0	40.0	32.0	41.0
23	37.18934278779803	39.0	36.0	40.0	32.0	41.0
24	37.11883595869224	39.0	36.0	40.0	31.0	41.0
25	37.05438823585399	39.0	36.0	40.0	31.0	41.0
26	36.785054014181696	39.0	36.0	40.0	31.0	41.0
27	36.65051634702327	38.0	36.0	40.0	30.0	41.0
28	36.520712892019226	38.0	35.0	40.0	30.0	41.0
29	36.38532813020511	38.0	35.0	40.0	30.0	41.0
30	36.29194689002046	38.0	35.0	40.0	30.0	41.0
31	36.24472088706991	38.0	35.0	40.0	30.0	41.0
32	36.13842288107362	38.0	35.0	40.0	30.0	41.0
33	36.10153428829772	38.0	35.0	40.0	30.0	41.0
34	36.01470994146481	38.0	35.0	40.0	29.0	41.0
35	35.945716461238284	38.0	35.0	40.0	29.0	41.0
36	35.90463427402084	38.0	35.0	40.0	29.0	41.0
37	35.67031266358921	38.0	34.0	40.0	28.0	41.0
38	35.52916480274116	38.0	34.0	40.0	27.0	41.0
39	35.47252082044449	38.0	34.0	40.0	27.0	41.0
40	35.35859896254699	38.0	34.0	40.0	27.0	41.0
41	35.20815495169657	38.0	34.0	40.0	26.0	41.0
42	35.12073383143768	38.0	34.0	40.0	26.0	41.0
43	34.18306001046971	37.0	33.0	40.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	0.0
8	0.0
9	0.0
10	1.0
11	2.0
12	1.0
13	8.0
14	6.0
15	18.0
16	39.0
17	59.0
18	116.0
19	212.0
20	438.0
21	782.0
22	1404.0
23	2320.0
24	3529.0
25	5496.0
26	7939.0
27	10847.0
28	15325.0
29	20122.0
30	26673.0
31	33455.0
32	42921.0
33	54676.0
34	71356.0
35	96382.0
36	136703.0
37	195043.0
38	230272.0
39	94504.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.20901346785323	20.594393946604484	12.90001427687622	24.296578308666064
2	18.8701280159901	22.273640127540094	34.91866939513634	23.93756246133346
3	20.706515014514824	24.724408699376575	29.649359920049495	24.919716366059106
4	15.278351496692524	16.258125922048254	34.51825060676724	33.94527197449198
5	13.988578499024412	37.84761814115072	34.37319754437729	13.790605815447579
6	34.18845476609717	35.362204349688284	15.926140960357873	14.52319992385666
7	28.09651168324371	31.222766858611333	22.15162042545091	18.529101032694044
8	26.390424975015463	34.51339646885261	20.440774758482842	18.655403797649075
9	26.364536239470805	14.375862561271594	20.263931851710844	38.99566934754676
10	16.712892019226196	27.35820682434683	33.26750107076572	22.661400085661256
11	34.99633560177033	22.302574596678244	21.985152048731738	20.715937752819684
12	20.961214486270404	26.395755008804073	29.353733403131393	23.289297101794126
13	29.00185599390853	20.499405130157523	26.49674011326322	24.00199876267073
14	22.583257983153285	21.731880264598104	25.61500023794794	30.069861514300673
15	25.153952315233425	28.4696140484462	23.26988055013563	23.106553086184743
16	24.94779422262409	27.246180935611285	24.5220577737591	23.28396706800552
17	23.287393518298195	27.36049112454195	25.924237376861942	23.42787798029791
18	22.669585494693763	25.752914862228142	27.89292342835388	23.684576214724217
19	23.744539094846047	26.126302764955028	27.587874173130917	22.541283967068004
20	24.174748964926472	25.149383714843193	27.644029886260885	23.03183743396945
21	24.128111169276163	26.247085137771855	26.509208585161563	23.115595107790416
22	24.057678579926712	25.73502117736639	26.8038833103317	23.403416932375194
23	23.101318231570932	26.142292866320847	27.16156664921715	23.594822252891067
24	23.899395612240042	26.52938657021844	26.71098843573026	22.86022938181126
25	23.964212630276496	25.696854328272973	26.994336839099603	23.344596202350925
26	23.60995574168372	26.667681911197832	26.737924142197688	22.984438204920764
27	24.277732832056344	25.644315423785276	26.37957454908866	23.69837719506972
28	23.035454242611717	26.103079046304668	27.516584971208303	23.344881739875316
29	22.966163803359823	26.491029362775425	27.289106743444535	23.253700090420214
30	22.746014372055395	27.14281635178223	27.320515871127398	22.790653405034977
31	23.473849521724645	26.55356208061676	26.834150287917	23.13843810974159
32	22.756484081283016	26.246894779422263	26.823870937038976	24.172750202255745
33	22.72536049112454	26.193499262361396	27.34107457288345	23.74006567363061
34	23.51848855470423	26.0193213724837	27.09065816399372	23.371531908818348
35	23.790986532146764	25.93499262361395	27.288726026745348	22.98529481749393
36	22.884404892209584	26.652929139104366	27.038499976205205	23.424165992480845
37	23.915100176081474	25.48584209774901	27.059344215485652	23.539713510683864
38	23.078189692095368	25.657830866606385	27.720649121972112	23.54333031932613
39	23.378479988578498	25.625565126350352	27.44167896064341	23.554275924427735
40	22.82149145766906	25.355636986627328	28.287917003759578	23.534954551944036
41	22.15390472564603	25.84028934469138	28.606767239328036	23.399038690334557
42	23.307285965830676	25.362870603911862	28.05168229191453	23.27816113834293
43	22.312568410031886	25.094084614286395	28.23642507019464	24.35692190548708
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	246.0
1	219.0
2	192.0
3	446.0
4	700.0
5	700.0
6	945.0
7	1190.0
8	1166.0
9	1142.0
10	1696.5
11	2251.0
12	2251.0
13	3696.0
14	5141.0
15	7218.5
16	9296.0
17	9255.5
18	9215.0
19	9215.0
20	10745.0
21	12275.0
22	12657.0
23	13039.0
24	14469.5
25	15900.0
26	15900.0
27	17922.5
28	19945.0
29	24674.5
30	29404.0
31	31822.5
32	34241.0
33	34241.0
34	37906.5
35	41572.0
36	45039.0
37	48506.0
38	54960.5
39	61415.0
40	61415.0
41	64482.0
42	67549.0
43	65345.0
44	63141.0
45	66258.0
46	69375.0
47	69375.0
48	71724.0
49	74073.0
50	75202.5
51	76332.0
52	79730.5
53	83129.0
54	83129.0
55	74267.5
56	65406.0
57	61649.5
58	57893.0
59	52631.5
60	47370.0
61	47370.0
62	43860.0
63	40350.0
64	35848.0
65	31346.0
66	27438.0
67	23530.0
68	23530.0
69	20093.0
70	16656.0
71	14322.5
72	11989.0
73	9474.5
74	6960.0
75	6960.0
76	5381.0
77	3802.0
78	3114.0
79	2426.0
80	1935.0
81	1444.0
82	1444.0
83	1109.0
84	774.0
85	690.5
86	607.0
87	537.0
88	467.0
89	467.0
90	375.0
91	283.0
92	168.5
93	54.0
94	37.5
95	21.0
96	21.0
97	11.0
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1050650.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.793636815815994
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.59596534655249	50.88480716054538
2	5.810993122909524	6.6005486592345575
3	1.5649172983937742	2.6663203407529186
4	0.7324558526064664	1.66395326706202
5	0.4149486165555497	1.178322051294059
6	0.2721352469874898	0.9273330229313929
7	0.20387325547760035	0.8105092539637032
8	0.1522074414739506	0.6915531321388895
9	0.11882747191791382	0.6073779855462814
>10	0.8371916235222308	9.709226046647048
>50	0.15246065003223755	6.118061821536621
>100	0.1367807577093966	14.634782602844613
>500	0.005895722212739519	2.0998263770765426
>1k	0.0013475936486261756	1.4073782784260338
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3174	0.302098700804264	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2941	0.27992195307666684	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2383	0.22681197354018942	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1730	0.1646599723980393	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	1220	0.1161185932517965	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	1202	0.11440536810545852	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	9.517917479655451E-5	0.0	1.9035834959310903E-4	0.0
3	0.0	9.517917479655451E-5	0.0	4.7589587398277254E-4	0.0
4	0.0	9.517917479655451E-5	0.0	7.614333983724361E-4	0.0
5	0.0	9.517917479655451E-5	0.0	8.566125731689907E-4	0.0
6	0.0	9.517917479655451E-5	0.0	8.566125731689907E-4	0.0
7	0.0	9.517917479655451E-5	0.0	8.566125731689907E-4	0.0
8	0.0	9.517917479655451E-5	0.0	0.0010469709227620997	0.0
9	0.0	9.517917479655451E-5	0.0	0.0019987626707276447	0.0
10	0.0	9.517917479655451E-5	0.0	0.0030457335934897444	0.0
11	0.0	9.517917479655451E-5	0.0	0.0038071669918621803	0.0
12	0.0	9.517917479655451E-5	0.0	0.005139675439013944	0.0
13	0.0	9.517917479655451E-5	0.0	0.005615571312996716	0.0
14	0.0	9.517917479655451E-5	0.0	0.006186646361776044	0.0
15	0.0	9.517917479655451E-5	0.0	0.007423975634131252	0.0
16	0.0	9.517917479655451E-5	0.0	0.00904202160567268	0.0
17	0.0	9.517917479655451E-5	0.0	0.011040784276400324	0.0
18	0.0	9.517917479655451E-5	0.0	0.011992576024365868	0.0
19	0.0	9.517917479655451E-5	0.0	0.013991338695093513	0.0
20	0.0	9.517917479655451E-5	0.0	0.015133488792652168	0.0
21	0.0	9.517917479655451E-5	0.0	0.018179222386141912	0.0
22	0.0	9.517917479655451E-5	0.0	0.023128539475562745	0.0
23	0.0	9.517917479655451E-5	0.0	0.030457335934897443	0.0
24	0.0	9.517917479655451E-5	0.0	0.04311616618283919	0.0
25	0.0	9.517917479655451E-5	0.0	0.0474944082234807	0.0
26	0.0	9.517917479655451E-5	0.0	0.05349069623566364	0.0
27	0.0	9.517917479655451E-5	0.0	0.06586398895921572	0.0
28	0.0	9.517917479655451E-5	0.0	0.09365630799980965	0.0
29	0.0	9.517917479655451E-5	0.0	0.13153761956883833	0.0
30	0.0	9.517917479655451E-5	0.0	0.19035834959310902	0.0
31	0.0	9.517917479655451E-5	0.0	0.3845238661780802	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTGTA	25	0.005496131	29.6	9
GGTATCA	1475	0.0	17.935593	1
TCTAGCG	115	6.4033884E-8	17.695652	28
AGCACCG	90	4.445372E-5	16.444445	5
TAGACTA	80	3.3822184E-4	16.1875	5
CCGATCT	80	3.3822184E-4	16.1875	9
AAGACGG	240	0.0	16.1875	5
TTAGAGT	175	1.3096724E-10	15.857143	4
TATACTG	105	9.341491E-6	15.857142	5
ATTAGAG	190	2.7284841E-11	15.578948	3
TATTCCG	95	7.058754E-5	15.578948	5
CTAGACA	85	5.363606E-4	15.235294	4
TGAACCG	85	5.363606E-4	15.235294	14
TCTAGAC	85	5.363606E-4	15.235294	3
TCAGTAG	135	3.9708902E-7	15.074073	3
AGAGTGT	210	9.094947E-12	14.97619	6
GAGTGTT	175	2.235538E-9	14.8	7
TAGGGTC	100	1.0932215E-4	14.8	4
ATAACTA	200	6.184564E-11	14.8	5
AGTACTA	75	0.0041040946	14.799999	5
>>END_MODULE