FastQCFastQC Report
Fri 10 Feb 2017
ERR1630607.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630607.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences410096
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT26510.6464340056962272No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT19970.486959151028052No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT16160.39405407514338103No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGT16030.39088408567749994No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT11650.28407982521165775TruSeq Adapter, Index 1 (95% over 21bp)
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG10740.26188989895048964No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9240.22531309742109162No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG8040.19605165619757328No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC7970.194344738792868No Hit
GAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTG7920.1931255120752214No Hit
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC7210.1758124926846397No Hit
GTCCTAAACTACTAAACCTGCATTAAAAATTTCGGTTGGGGCG7100.17313019390581716No Hit
GATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTT7090.17288634856228785No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG7090.17288634856228785No Hit
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC6820.1663025242869962No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATG6640.16191330810346846No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC6540.15947485466817526No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG6410.15630486520229409No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG6400.15606101985876478No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGAT6360.1550856384846475No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC5990.14606336077406265No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATG5900.14386875268229876No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC5850.1426495259646522No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA5460.1331395575670087No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT5400.13167648550583277No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT5360.1307011041317155No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT5310.12948187741406889No Hit
GTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTG5310.12948187741406889No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT5260.12826265069642231No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC5230.12753111466583433No Hit
GGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTC5150.12558035191759978No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT4960.1209472903905427No Hit
CCATCGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAT4920.11997190901642542No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA4910.1197280636728961No Hit
GTTTACGACCTCGATGTTGGATCAGGACATCCCGATGGTGCAG4900.11948421832936677No Hit
ATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCA4900.11948421832936677No Hit
GGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAAC4840.11802114626819087No Hit
TCCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGA4830.11777730092466154No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC4750.11582653817642699No Hit
CTACTAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCGGA4710.1148511568023097No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG4700.11460731145878039No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGAC4640.11314423939760446No Hit
GTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGT4540.11070578596231126No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT4480.10924271390113534No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTC4450.10851117787054737No Hit
GTCCTACGTGATCTGAGTTCAGACCGGAGTAATCCAGGTCGGT4410.10753579649643011No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA4320.10534118840466622No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG4240.10339042565643167No Hit
CTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGG4160.1014396629081971No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTCTA200.001840555837.029
TACATAC200.001840555837.03
GTTACGG200.001840555837.017
GTCTAGA250.00549227229.631
CCTTGAG250.00549227229.62
CCGTCTT1700.029.38235337
GCCGTCT1850.027.036
CGTCTTC451.3211854E-424.66666637
AGTTCCT701.916651E-723.78571516
CTGCGCG400.001929080723.1259
ATATAAT400.001929080723.1251
TATACAC3450.023.057973
ACCTGTG855.160291E-821.7647068
TTTAGGA1450.021.6896552
TTATACA3600.021.5833322
GCCGAGT806.93768E-720.812512
GACGGCA450.003821675420.55555531
ACCGTTA450.003821675420.5555558
GCATCTA450.003821675420.55555535
TCCGACG555.135342E-420.18181828
Produced by FastQC (version 0.11.5)