FastQCFastQC Report
Fri 10 Feb 2017
ERR1630606.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630606.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1418963
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT37990.26773073011769866No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT31560.2224159474207573No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT28430.20035758508149967No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18700.13178638202687457No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGGT450.003826243820.5555553
TCTAGCG2250.018.91111228
CAAGACG3000.017.8833334
CTATACC1258.581992E-917.7600024
CTAGCGG2500.017.76000229
TCTAATA1301.3938916E-817.0769232
TCTATAC650.00158018117.0769233
TAATACT1757.2759576E-1216.9142864
AAGACGG2850.016.8771935
GAACCGT1005.879745E-616.6500026
CGCAATA2600.016.36538536
CTAGTAG803.383265E-416.18753
CGCAAGA3000.016.0333332
TACACGG957.061538E-515.5789485
GACGGAC2850.015.5789477
GGTATCA18800.015.5478731
CTTGCGC3100.015.51612953
CGGTCCA2950.015.05084710
CGGCGCA3850.014.89610433
ATTATAC1252.9597431E-614.8000013
Produced by FastQC (version 0.11.5)