##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630606.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1418963
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.986005977604773	33.0	31.0	34.0	30.0	34.0
2	32.15417949587128	33.0	31.0	34.0	30.0	34.0
3	32.25939295104946	34.0	31.0	34.0	30.0	34.0
4	35.84720602299003	37.0	35.0	37.0	35.0	37.0
5	35.81888745513449	37.0	35.0	37.0	35.0	37.0
6	35.84891078907625	37.0	35.0	37.0	35.0	37.0
7	35.82385375799087	37.0	35.0	37.0	35.0	37.0
8	35.80741358301802	37.0	35.0	37.0	35.0	37.0
9	37.54427141511089	39.0	37.0	39.0	35.0	39.0
10	37.441675364332966	39.0	37.0	39.0	34.0	39.0
11	37.549866346056945	39.0	37.0	39.0	35.0	39.0
12	37.47764458974618	39.0	37.0	39.0	35.0	39.0
13	37.52158794838202	39.0	37.0	39.0	35.0	39.0
14	38.79713001677986	40.0	38.0	41.0	35.0	41.0
15	38.77152469796605	40.0	38.0	41.0	35.0	41.0
16	38.747731265720105	40.0	38.0	41.0	35.0	41.0
17	38.731860520676015	40.0	38.0	41.0	35.0	41.0
18	38.70769428096434	40.0	38.0	41.0	35.0	41.0
19	38.74618083769626	40.0	38.0	41.0	34.0	41.0
20	38.72696116812066	40.0	38.0	41.0	34.0	41.0
21	38.64942990056823	40.0	38.0	41.0	34.0	41.0
22	38.59225998140896	40.0	38.0	41.0	34.0	41.0
23	38.56871461764683	40.0	38.0	41.0	34.0	41.0
24	38.51449121647288	40.0	38.0	41.0	34.0	41.0
25	38.47263106930907	40.0	38.0	41.0	34.0	41.0
26	38.28492920534221	40.0	38.0	41.0	34.0	41.0
27	38.17211231018709	40.0	38.0	41.0	33.0	41.0
28	38.07399417743803	40.0	38.0	41.0	33.0	41.0
29	37.97100276751402	40.0	37.0	41.0	33.0	41.0
30	37.903496426615774	40.0	37.0	41.0	33.0	41.0
31	37.866164938761614	40.0	37.0	41.0	33.0	41.0
32	37.76480006878263	40.0	37.0	41.0	33.0	41.0
33	37.729690626182645	40.0	37.0	41.0	33.0	41.0
34	37.65899745095538	40.0	37.0	41.0	32.0	41.0
35	37.603431520060774	40.0	37.0	41.0	32.0	41.0
36	37.56752572124854	40.0	37.0	41.0	32.0	41.0
37	37.39252468175703	40.0	36.0	41.0	32.0	41.0
38	37.27902559827142	40.0	36.0	41.0	31.0	41.0
39	37.22210586181599	40.0	36.0	41.0	31.0	41.0
40	37.123719927862815	39.0	36.0	41.0	31.0	41.0
41	36.99825999691324	39.0	36.0	41.0	31.0	41.0
42	36.91218587094942	39.0	35.0	41.0	31.0	41.0
43	36.03802706624486	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	4.0
11	3.0
12	4.0
13	2.0
14	2.0
15	1.0
16	5.0
17	17.0
18	42.0
19	92.0
20	225.0
21	400.0
22	750.0
23	1349.0
24	2096.0
25	3518.0
26	5238.0
27	8113.0
28	11218.0
29	15333.0
30	20761.0
31	27340.0
32	35640.0
33	45957.0
34	60523.0
35	81026.0
36	112891.0
37	172872.0
38	315485.0
39	498055.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.645676455270504	18.753202162424248	11.918281167303164	25.682840215002084
2	19.24778870203099	20.88024846313822	34.05324874573897	25.81871408909182
3	20.825983482303627	23.004193907804503	28.730488391874907	27.43933421801696
4	14.935977893715341	15.166568825261828	33.530684027701916	36.36676925332091
5	14.802852505667872	36.54337710003714	33.82794336427377	14.825827030021218
6	35.7386344816602	33.652674523578135	15.484688466154509	15.124002528607159
7	29.585619921026833	29.780762430028123	21.17440694366238	19.45921070528266
8	27.395217493338446	32.725518565318474	20.018633325886583	19.860630615456497
9	26.87899543539895	13.766673267731436	19.437786608953157	39.916544687916456
10	17.369797521147486	26.23514496149653	32.42325557466967	23.971801942686312
11	36.04498496437187	21.491539948539884	21.012951007179186	21.450524079909062
12	22.146454840612474	25.12052816035372	28.27896146693043	24.454055532103375
13	29.945248748557923	19.613760189659633	25.26725503061038	25.173736031172062
14	23.454240878726225	20.888282499261784	24.495917088747206	31.16155953326479
15	26.246209379666702	27.197467446297047	22.10219716793179	24.454126006104456
16	26.005188295959798	25.888130980159456	23.557485290314123	24.549195433566627
17	24.354969086579427	26.020974472202585	24.90396155502293	24.72009488619506
18	23.94762936031454	24.508813830945556	26.68420529640308	24.85935151233683
19	24.608041224471673	25.03736883907473	26.27587893412302	24.078711002330575
20	25.112987442237745	24.12134777298633	26.277781732152285	24.48788305262364
21	25.354572317953323	24.88098703066958	25.39178259052562	24.37265806085148
22	25.34463548380049	24.460045822195507	25.439634437261578	24.755684256742423
23	24.309020037872727	24.909881371113975	25.986301263669315	24.794797327343986
24	24.712201798073664	25.34886392386553	25.701938669295814	24.236995608764992
25	25.03321087301078	24.586617128142173	25.685659175045437	24.694512823801606
26	24.730595512356558	25.458098625545556	25.66324844270076	24.148057419397123
27	25.393051122545124	24.462019094225855	25.16816858508643	24.97676119814259
28	24.240589782820273	24.87281204654385	26.17319831454379	24.71339985609209
29	23.887374089387812	25.30904611325313	26.181232350667354	24.622347446691702
30	23.72753905492955	26.04218714652884	26.211747593136682	24.01852620540493
31	24.66068530328134	25.503342934241413	25.406582130753236	24.429389631724014
32	23.968278242632117	25.22229261791886	25.67374906886226	25.13568007058676
33	23.827118818461088	25.007910706621665	26.14782767415359	25.017142800763658
34	24.697261309843878	24.853643118249032	25.90758180445861	24.541513767448482
35	24.680699919589163	24.908260469089047	26.41746120230055	23.993578409021236
36	23.867429947081074	25.548798664940524	25.97537779350131	24.608393594477093
37	24.79402211333206	24.561316961753054	25.97439115748614	24.67026976742875
38	23.897310923540644	24.642080166995193	26.799077918169818	24.66153099129435
39	24.29922415172207	24.57280422392973	26.36714276552666	24.760828858821547
40	23.608578941099946	24.50796814293255	27.194859908256948	24.688593007710562
41	23.111102967448762	24.72826987032079	27.618338180770042	24.542288981460405
42	24.09238295854085	24.384991011041162	27.269280453401535	24.253345577016454
43	23.19947736480796	23.969617248652714	27.442716970068986	25.388188416470335
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	92.0
1	107.5
2	123.0
3	336.0
4	549.0
5	549.0
6	809.5
7	1070.0
8	1056.5
9	1043.0
10	1488.0
11	1933.0
12	1933.0
13	3419.0
14	4905.0
15	6811.0
16	8717.0
17	8731.5
18	8746.0
19	8746.0
20	10412.0
21	12078.0
22	12117.5
23	12157.0
24	13873.0
25	15589.0
26	15589.0
27	18114.0
28	20639.0
29	25775.5
30	30912.0
31	34553.5
32	38195.0
33	38195.0
34	43027.5
35	47860.0
36	52903.5
37	57947.0
38	65380.0
39	72813.0
40	72813.0
41	76975.0
42	81137.0
43	80450.5
44	79764.0
45	84215.0
46	88666.0
47	88666.0
48	92839.5
49	97013.0
50	100541.5
51	104070.0
52	110040.0
53	116010.0
54	116010.0
55	108120.0
56	100230.0
57	96252.5
58	92275.0
59	85010.0
60	77745.0
61	77745.0
62	72730.5
63	67716.0
64	60886.0
65	54056.0
66	47823.0
67	41590.0
68	41590.0
69	35561.0
70	29532.0
71	25956.0
72	22380.0
73	17639.5
74	12899.0
75	12899.0
76	10052.0
77	7205.0
78	5897.5
79	4590.0
80	3708.5
81	2827.0
82	2827.0
83	2124.5
84	1422.0
85	1251.5
86	1081.0
87	968.5
88	856.0
89	856.0
90	655.0
91	454.0
92	256.0
93	58.0
94	35.5
95	13.0
96	13.0
97	8.5
98	4.0
99	3.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1418963.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.92966346116691
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.31966221365401	49.710789833660954
2	7.640038490588676	8.698896401991497
3	1.9250772315939242	3.2878199679419096
4	0.8502986994276601	1.9362887519953842
5	0.47422295422707317	1.3498676594853816
6	0.290092579951384	0.9908923769528366
7	0.21102365193095013	0.840945384074279
8	0.15627741688383617	0.7117456599821821
9	0.11841413825637023	0.6067149335981322
>10	0.7559490855735732	8.636961542681835
>50	0.12520029664437116	5.078301850270919
>100	0.12479389953762735	13.98681342969121
>500	0.007954970427160877	2.9921512779586528
>1k	9.943713033951096E-4	1.1718109297149175
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3799	0.26773073011769866	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3156	0.2224159474207573	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2843	0.20035758508149967	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1870	0.13178638202687457	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.4094800216778028E-4	0.0
2	0.0	1.4094800216778028E-4	0.0	0.0011275840173422423	0.0
3	0.0	1.4094800216778028E-4	0.0	0.002184694033600594	0.0
4	0.0	1.4094800216778028E-4	0.0	0.003664648056362287	0.0
5	0.0	2.114220032516704E-4	0.0	0.0039465440606978475	0.0
6	0.0	2.114220032516704E-4	0.0	0.0040170180617817375	0.0
7	0.0	2.114220032516704E-4	0.0	0.004580810070452859	0.0
8	0.0	2.114220032516704E-4	0.0	0.0050036540769562	0.0
9	0.0	2.114220032516704E-4	0.0	0.008245458126815146	0.0
10	0.0	2.8189600433556057E-4	0.0	0.012403424190764664	0.0
11	0.0	2.8189600433556057E-4	0.0	0.013531008208106906	0.0
12	0.0	2.8189600433556057E-4	0.0	0.014517644223281369	0.0
13	0.0	2.8189600433556057E-4	0.0	0.01536333223628805	0.0
14	0.0	2.8189600433556057E-4	0.0	0.016561390254714183	0.0
15	0.0	2.8189600433556057E-4	0.0	0.018534662285063105	0.0
16	0.0	2.8189600433556057E-4	0.0	0.02008509030890869	0.0
17	0.0	2.8189600433556057E-4	0.0	0.023185946356599856	0.0
18	0.0	2.8189600433556057E-4	0.0	0.024384004375025987	0.0
19	0.0	3.5237000541945066E-4	0.0	0.02670964641079436	0.0
20	0.0	3.5237000541945066E-4	0.0	0.029458132453066076	0.0
21	0.0	3.5237000541945066E-4	0.0	0.03269993650292502	0.0
22	0.0	3.5237000541945066E-4	0.0	0.03883117459722347	0.0
23	0.0	4.228440065033408E-4	0.0	0.04594904870669637	0.0
24	0.0	4.228440065033408E-4	0.0	0.05588588285952488	0.0
25	0.0	4.228440065033408E-4	0.0	0.05898673890721604	0.0
26	0.0	4.228440065033408E-4	0.0	0.06469513299501115	0.0
27	0.0	4.228440065033408E-4	0.0	0.07449101914567187	0.0
28	0.0	4.228440065033408E-4	0.0	0.09380089544265777	0.0
29	0.0	4.228440065033408E-4	0.0	0.12480945591956943	0.0
30	0.0	4.228440065033408E-4	0.0	0.17096992662951746	0.0
31	0.0	4.228440065033408E-4	0.0	0.31896532890568674	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTAGGT	45	0.0038262438	20.555555	3
TCTAGCG	225	0.0	18.911112	28
CAAGACG	300	0.0	17.883333	4
CTATACC	125	8.581992E-9	17.760002	4
CTAGCGG	250	0.0	17.760002	29
TCTAATA	130	1.3938916E-8	17.076923	2
TCTATAC	65	0.001580181	17.076923	3
TAATACT	175	7.2759576E-12	16.914286	4
AAGACGG	285	0.0	16.877193	5
GAACCGT	100	5.879745E-6	16.650002	6
CGCAATA	260	0.0	16.365385	36
CTAGTAG	80	3.383265E-4	16.1875	3
CGCAAGA	300	0.0	16.033333	2
TACACGG	95	7.061538E-5	15.578948	5
GACGGAC	285	0.0	15.578947	7
GGTATCA	1880	0.0	15.547873	1
CTTGCGC	310	0.0	15.5161295	3
CGGTCCA	295	0.0	15.050847	10
CGGCGCA	385	0.0	14.896104	33
ATTATAC	125	2.9597431E-6	14.800001	3
>>END_MODULE