FastQCFastQC Report
Fri 10 Feb 2017
ERR1630605.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630605.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2037607
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT53400.2620721267643859No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT50850.24955744655372702No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT40400.19827179627867395No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24910.12225124864608336No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24100.020.7261411
AAGACGG5100.018.1372555
CGAACGA2350.018.10638216
GATCCGT852.724789E-517.4117646
GACGGAC5300.017.1037737
ACGAACG2500.017.0215
ACGGACC5300.016.7547178
CTTATAC11200.016.187537
CGGACCA5500.016.1454549
TATACAG1851.8189894E-1116.05
ATAACGA2850.015.57894812
CCTATAC1802.0190782E-1015.4166663
CGCAAGA5900.015.3644072
GAGCGAA5800.015.31034516
CAAGACG6350.015.1496074
TCTTATA17500.015.11714337
AGACGGA5900.015.0508476
ATACCGT3450.015.0144926
GCGGGTA4700.014.95744623
TAACGAA2850.014.92982613
Produced by FastQC (version 0.11.5)