FastQCFastQC Report
Fri 10 Feb 2017
ERR1630604.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630604.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences887129
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT65180.734729672911155No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT57270.6455656392700497No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT51560.5812007047453076No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30160.33997310424977656No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGT14550.1640122237014008No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA13220.14902004105378136No Hit
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT11190.1261372359600464RNA PCR Primer, Index 27 (95% over 23bp)
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC10560.11903567575854243No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC9060.10212719908829493No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8930.1006617977768735No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAACG405.938625E-527.7513
GCCGTCT2150.025.81395336
ACGCATT451.3228798E-424.66666617
ATGCCGT2300.023.32608834
CGCATTT502.7014286E-422.218
AACGCAT502.7014286E-422.216
TATGCCG2500.022.19999933
TGCCGTC2550.021.76470635
GCGAACT1201.0913936E-1121.58333228
GGACCGT450.003825130620.5555556
CCGTCTT3000.019.11666737
TACTGGT1551.8189894E-1219.0967737
CGAACTA1306.9485395E-1018.529
TAGACAG1002.8730938E-718.55
CGAATTA609.234552E-418.515
ACGCTTC902.1510896E-618.531
CAGCTTA1205.167749E-918.59
TCTGTCG1451.546141E-1017.8620688
AAGTTGT1156.399932E-817.69565229
CGTATGC3200.017.3437531
Produced by FastQC (version 0.11.5)