##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1630604.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 887129 Sequences flagged as poor quality 0 Sequence length 43 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.046092507403095 33.0 31.0 34.0 30.0 34.0 2 32.211279306617186 33.0 31.0 34.0 30.0 34.0 3 32.29841206859431 34.0 31.0 34.0 30.0 34.0 4 35.883277403849945 37.0 35.0 37.0 35.0 37.0 5 35.85371687770324 37.0 35.0 37.0 35.0 37.0 6 35.945726044352064 37.0 35.0 37.0 35.0 37.0 7 35.89110377408472 37.0 35.0 37.0 35.0 37.0 8 35.903588993258026 37.0 35.0 37.0 35.0 37.0 9 37.595807374124846 39.0 37.0 39.0 35.0 39.0 10 37.51909361547193 39.0 37.0 39.0 35.0 39.0 11 37.62681188417919 39.0 37.0 39.0 35.0 39.0 12 37.574317827508736 39.0 37.0 39.0 35.0 39.0 13 37.6144179707799 39.0 37.0 39.0 35.0 39.0 14 38.87959924655828 40.0 38.0 41.0 35.0 41.0 15 38.871161916699826 40.0 38.0 41.0 35.0 41.0 16 38.817326454213536 40.0 38.0 41.0 35.0 41.0 17 38.824094353808746 40.0 38.0 41.0 35.0 41.0 18 38.84251332106154 40.0 38.0 41.0 35.0 41.0 19 38.918276823325584 40.0 38.0 41.0 35.0 41.0 20 38.9058513474365 40.0 38.0 41.0 35.0 41.0 21 38.86152972115668 40.0 38.0 41.0 35.0 41.0 22 38.77242317633625 40.0 38.0 41.0 35.0 41.0 23 38.712691164419155 40.0 38.0 41.0 35.0 41.0 24 38.66401053285373 40.0 38.0 41.0 34.0 41.0 25 38.59820048718957 40.0 38.0 41.0 34.0 41.0 26 38.46874918980216 40.0 38.0 41.0 34.0 41.0 27 38.31688176127711 40.0 38.0 41.0 34.0 41.0 28 38.209716963372856 40.0 38.0 41.0 34.0 41.0 29 38.12193604312338 40.0 38.0 41.0 33.0 41.0 30 38.008889349801436 40.0 37.0 41.0 33.0 41.0 31 37.93863913816367 40.0 37.0 41.0 33.0 41.0 32 37.80570807627752 40.0 37.0 41.0 33.0 41.0 33 37.694221471736356 40.0 37.0 41.0 33.0 41.0 34 37.61629030276318 40.0 37.0 41.0 33.0 41.0 35 37.53609790684331 40.0 37.0 41.0 33.0 41.0 36 37.47474493562943 40.0 36.0 41.0 33.0 41.0 37 37.309732857340926 40.0 36.0 41.0 32.0 41.0 38 37.12656671126747 40.0 36.0 41.0 31.0 41.0 39 37.01287862306384 40.0 36.0 41.0 31.0 41.0 40 36.806289727874976 39.0 35.0 41.0 31.0 41.0 41 36.70439361130118 39.0 35.0 41.0 30.0 41.0 42 36.517864932833895 39.0 35.0 41.0 30.0 41.0 43 35.707412338002705 38.0 35.0 40.0 27.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 4.0 11 3.0 12 2.0 13 2.0 14 1.0 15 2.0 16 5.0 17 12.0 18 24.0 19 49.0 20 121.0 21 223.0 22 414.0 23 734.0 24 1252.0 25 2029.0 26 3198.0 27 4859.0 28 6820.0 29 9757.0 30 13088.0 31 16819.0 32 21614.0 33 28041.0 34 37092.0 35 49092.0 36 69556.0 37 111980.0 38 196867.0 39 313468.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.49082376971106 19.629726905557142 12.462674537750429 23.416774786981374 2 19.538533854715606 21.369270985392202 34.16233715727927 24.929858002612924 3 21.71814922068831 23.882885127191198 29.33665791559063 25.06230773652986 4 14.104713068787063 15.566281792163258 34.60951000361842 35.71949513543126 5 13.772179694272197 38.03956358094482 33.813120752449755 14.375135972333222 6 34.21588066673505 34.038905277586466 15.970168938226573 15.775045117451917 7 27.92446194409156 31.632490877876833 20.997735391357967 19.445311786673642 8 28.814411432835584 31.14439951799569 20.16944548087144 19.87174356829728 9 27.07272561262229 12.977030398059359 21.11271303271565 38.837530956602706 10 18.254165966843605 26.914011378277568 31.86357339237022 22.968249262508607 11 34.329731076314715 22.153035240647075 20.56352571046601 22.953707972572197 12 22.13950845931088 27.325789146786995 27.76112605945697 22.77357633444516 13 30.585292556099507 19.875914325875943 25.748679166164106 23.79011395186044 14 23.324679950717428 21.932435981689245 25.946959235917213 28.795924831676118 15 27.081856190024222 26.335966922510707 24.081841536011108 22.50033535145396 16 25.039424931436127 26.00354627117364 25.17536908386492 23.781659713525315 17 22.41759653894755 26.61608401934781 26.35670798722621 24.609611454478436 18 22.57631077329227 23.97723442701118 28.734603422952016 24.71185137674453 19 23.065867534484838 25.414680390337818 28.57014030653941 22.94931176863793 20 24.6549261719547 23.821112825755893 28.817116789102826 22.706844213186585 21 25.462475017725723 24.63046524237174 27.294677549713743 22.6123821901888 22 24.93391603701378 24.589884898363145 27.69969192755507 22.776507137068002 23 23.93857037702521 24.648726397175608 27.85074098580928 23.5619622399899 24 23.238221273343562 25.53687231507481 27.658773414013073 23.566132997568563 25 23.481026998328318 25.394390218333523 27.690448626975332 23.43413415636283 26 23.519240155603075 25.4950520161104 27.46635494950565 23.51935287878088 27 23.54223568387461 25.090150361446874 27.917585830245656 23.450028124432862 28 22.808745965919275 25.03671957516889 28.409171608638655 23.745362850273185 29 22.255162439735372 25.697277397086555 28.920596666324737 23.126963496853335 30 22.338464868130792 26.53210525188558 28.706197182146003 22.42323269783763 31 23.161907681971844 26.91209508425494 27.15197000661685 22.774027227156367 32 22.09937900801349 26.107589764284562 28.103917243151784 23.68911398455016 33 21.723559933222788 25.780805271837583 28.89669935263079 23.59893544230884 34 22.53539225975027 25.42358552138415 28.609367972414386 23.431654246451192 35 22.03490135031095 25.741577605962608 28.79750295616534 23.42601808756111 36 22.06714017916222 26.971387475778606 27.661816939813715 23.29965540524546 37 22.895542812826545 25.901644518440946 28.042370388072086 23.160442280660423 38 22.16081313991539 25.590753994064 28.506338987903675 23.742093878116936 39 22.326065318572606 25.914833130243743 28.579496330296944 23.179605220886703 40 21.26770740219292 25.314356762094352 29.592877698733783 23.825058136978953 41 21.25620963805715 25.59030310135279 29.271391195643474 23.882096064946587 42 21.42225087895898 25.54803190967717 29.709433464580687 23.32028374678316 43 20.76980912584303 24.797520991873785 29.40102285011537 25.031647032167815 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 26.0 1 67.0 2 108.0 3 432.5 4 757.0 5 757.0 6 1184.0 7 1611.0 8 1651.0 9 1691.0 10 2325.5 11 2960.0 12 2960.0 13 5200.5 14 7441.0 15 10400.0 16 13359.0 17 12636.5 18 11914.0 19 11914.0 20 13183.5 21 14453.0 22 11893.5 23 9334.0 24 10529.0 25 11724.0 26 11724.0 27 13561.0 28 15398.0 29 17563.5 30 19729.0 31 22937.5 32 26146.0 33 26146.0 34 29501.0 35 32856.0 36 35780.0 37 38704.0 38 42185.0 39 45666.0 40 45666.0 41 48238.5 42 50811.0 43 52065.0 44 53319.0 45 53817.5 46 54316.0 47 54316.0 48 56996.0 49 59676.0 50 59180.0 51 58684.0 52 60760.0 53 62836.0 54 62836.0 55 60472.5 56 58109.0 57 56729.0 58 55349.0 59 52155.0 60 48961.0 61 48961.0 62 44825.0 63 40689.0 64 35774.0 65 30859.0 66 26953.0 67 23047.0 68 23047.0 69 19193.5 70 15340.0 71 12682.0 72 10024.0 73 7388.5 74 4753.0 75 4753.0 76 3693.5 77 2634.0 78 2129.5 79 1625.0 80 1272.5 81 920.0 82 920.0 83 725.5 84 531.0 85 449.5 86 368.0 87 295.5 88 223.0 89 223.0 90 183.5 91 144.0 92 83.0 93 22.0 94 15.5 95 9.0 96 9.0 97 6.0 98 3.0 99 1.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 43 887129.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.01023948859963 #Duplication Level Percentage of deduplicated Percentage of total 1 86.61565151703482 54.57672945549452 2 8.896313920485492 11.21117741391107 3 1.9377528035894396 3.6629480467162794 4 0.6845196439640486 1.725269868033026 5 0.3667007692392785 1.1552951645210325 6 0.22213635586663086 0.8398118989368721 7 0.16023025349425649 0.7067302652194491 8 0.11924143068115688 0.6010744883346356 9 0.10025633303428533 0.5685457999265209 >10 0.7378583682004793 9.3283579274253 >50 0.09481671097537708 4.119146290768468 >100 0.05950352397401926 7.1022724267480895 >500 0.0035845496148282623 1.537677548523368 >1k 8.961374037070656E-4 0.8998395054813599 >5k 5.376824422242393E-4 1.9651238999599827 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 6518 0.734729672911155 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT 5727 0.6455656392700497 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 5156 0.5812007047453076 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3016 0.33997310424977656 No Hit CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGT 1455 0.1640122237014008 No Hit GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA 1322 0.14902004105378136 No Hit TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT 1119 0.1261372359600464 RNA PCR Primer, Index 27 (95% over 23bp) GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC 1056 0.11903567575854243 No Hit TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC 906 0.10212719908829493 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 893 0.1006617977768735 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 2.2544635560330008E-4 0.0 2 0.0 0.0 0.0 0.0034944185118511513 0.0 3 0.0 0.0 0.0 0.006650667490297352 0.0 4 0.0 0.0 0.0 0.010595978713355103 0.0 5 0.0 0.0 0.0 0.011610487313569954 0.0 6 0.0 0.0 0.0 0.011835933669173255 0.0 7 0.0 0.0 0.0 0.013188611802793055 0.0 8 0.0 0.0 0.0 0.013526781336198005 0.0 9 0.0 0.0 0.0 0.020741064715503608 0.0 10 0.0 0.0 0.0 0.02772990173920591 0.0 11 0.0 0.0 0.0 0.030886150717652112 0.0 12 0.0 0.0 0.0 0.03336606062928841 0.0 13 0.0 0.0 0.0 0.03562052418532141 0.0 14 0.0 0.0 0.0 0.03764954138575111 0.0 15 0.0 0.0 0.0 0.041143959897602264 0.0 16 0.0 0.0 0.0 0.04892185916591612 0.0 17 0.0 0.0 0.0 0.05782699021224647 0.0 18 0.0 0.0 0.0 0.06087051601289102 0.0 19 0.0 0.0 0.0 0.06853569210340323 0.0 20 0.0 0.0 0.0 0.07473546688249398 0.0 21 0.0 0.0 0.0 0.08544416877365073 0.0 22 0.0 0.0 0.0 0.1071997420893692 0.0 23 0.0 0.0 0.0 0.1437220516971038 0.0 24 0.0 0.0 0.0 0.18836043010655723 0.0 25 0.0 0.0 0.0 0.20053453330913543 0.0 26 0.0 0.0 0.0 0.21890841129080438 0.0 27 0.0 0.0 0.0 0.23683139656126673 0.0 28 0.0 0.0 0.0 0.2648994678338776 0.0 29 0.0 0.0 0.0 0.3103269084879426 0.0 30 0.0 0.0 0.0 0.38258246545880026 0.0 31 0.0 0.0 0.0 0.6218937719317033 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTAACG 40 5.938625E-5 27.75 13 GCCGTCT 215 0.0 25.813953 36 ACGCATT 45 1.3228798E-4 24.666666 17 ATGCCGT 230 0.0 23.326088 34 CGCATTT 50 2.7014286E-4 22.2 18 AACGCAT 50 2.7014286E-4 22.2 16 TATGCCG 250 0.0 22.199999 33 TGCCGTC 255 0.0 21.764706 35 GCGAACT 120 1.0913936E-11 21.583332 28 GGACCGT 45 0.0038251306 20.555555 6 CCGTCTT 300 0.0 19.116667 37 TACTGGT 155 1.8189894E-12 19.096773 7 CGAACTA 130 6.9485395E-10 18.5 29 TAGACAG 100 2.8730938E-7 18.5 5 CGAATTA 60 9.234552E-4 18.5 15 ACGCTTC 90 2.1510896E-6 18.5 31 CAGCTTA 120 5.167749E-9 18.5 9 TCTGTCG 145 1.546141E-10 17.862068 8 AAGTTGT 115 6.399932E-8 17.695652 29 CGTATGC 320 0.0 17.34375 31 >>END_MODULE