FastQCFastQC Report
Fri 10 Feb 2017
ERR1630603.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630603.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences870912
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT108001.2400793650793651No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT102991.1825534611992945No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT82640.9488903586125808No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46400.532774838330394No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA38700.44436177248677255No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC32270.37053112139917693No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC31290.35927854938271603No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA26770.3073789315108759No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC26470.3039342666078777No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC22690.2605314888300999No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG20160.23148148148148145No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC19990.2295295047031158No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC19740.22665895061728397No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC19450.22332910787771898No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC18860.21655460023515577No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT17070.19600143298059966No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG16860.19359016754850086No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG15840.18187830687830686No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT15520.17820399764844208No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC14680.16855893592004703No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAAT14420.1655735596707819No Hit
GTACATGGGCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCAC14310.16431051587301587No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG14000.16075102880658437No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTC13470.15466545414462082No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG13370.15351723251028807No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG13350.15328758818342153No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC13240.1520245443856555No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT12890.1480057686654909No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCT12630.14502039241622575No Hit
TAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGC12410.1424943048206937No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCAT12340.14169054967666078No Hit
GACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTC12240.14054232804232802No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC12160.13962375073486186No Hit
GTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACAC11920.13686801881246324No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11870.1362939079952969No Hit
GAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGG11780.13526050852439742No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTG11660.13388264256319812No Hit
AGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGG11500.13204548794826573No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC11160.1281415343915344No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCT11040.1267636684303351No Hit
TCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGC10680.1226300705467372No Hit
GAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTG10560.12125220458553791No Hit
GCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGC10420.11964469429747207No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGA10390.11930022780717224No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAG10320.11849647266313933No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGT10160.11665931804820695No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG9900.11367394179894179No Hit
GTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGA9820.11275536449147561No Hit
CGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA9750.11195160934744268No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGAC9730.11172196502057612No Hit
GAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGC9540.10954034391534391No Hit
CACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGG9370.10758836713697824No Hit
GCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGC9330.10712907848324515No Hit
GCGTGTGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGA9300.10678461199294532No Hit
ACGCAGAGTACATGGGCAACTTCTTAGAGGGACAAGTGGCGTT9280.10655496766607878No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG9260.10632532333921224No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC9200.1056363903586126No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAAC9120.10471781305114639No Hit
ACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAG9110.1046029908877131No Hit
GGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCC8980.10311030276308054No Hit
TTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTA8910.10230654761904763No Hit
GTCCTAAACTACTAAACCTGCATTAAAAATTTCGGTTGGGGCG8880.10196208112874779No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA8810.10115832598471487No Hit
GGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCGCGGGTAACC8740.10035457084068196No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGGAC303.5987687E-430.8333343
TACGTAC303.5987687E-430.83333410
TTACCGG250.005495620429.631
CTACGTA358.867664E-426.428579
TCTGTCG1000.025.9000028
CTGTCGC1051.8189894E-1224.6666669
GTTACTT701.9218169E-723.78571328
ATGTTAC701.9218169E-723.78571326
TCAAAAG400.001930813223.1250023
GTACATA400.001930813223.12500213
CGTACAT400.001930813223.12500212
ACGTACA400.001930813223.12500211
GGACCCA400.001930813223.1250026
TGTCCAG400.001930813223.1250025
ACAGGCG1300.022.7692325
ACAGTAA502.7013733E-422.219
GCCGTCT502.7013733E-422.236
GAGTTGA753.7386235E-722.1999999
GCAGTCG4450.022.0337099
TAGACTA1107.4578566E-1121.8636365
Produced by FastQC (version 0.11.5)