FastQCFastQC Report
Fri 10 Feb 2017
ERR1630602.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630602.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1270864
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT27970.2200864923390701No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT23050.18137267244960908No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT18210.14328834556648076No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG1900.022.3947375
GGTATCA11000.020.8545461
AGTCGGT1251.8189894E-1120.72000111
CGGACTA555.1435054E-420.18181824
TAATACT2550.018.8627454
CCAATAC1205.175025E-918.53
TAGCCTA701.2193261E-418.55
GACGGAC2000.018.57
CTAGGTA500.0070352818.54
TTAATAC2200.017.659093
GCAGTCG1603.45608E-1117.343759
CAGTCGG1502.5102054E-1017.26666610
TCGCCAT1950.017.07692313
GTATAGA1107.8091944E-716.8181821
CGCAAAT1107.8091944E-716.81818223
GAGCGAA2000.016.65000216
CGAACTA1251.6580088E-716.2829
CAAGACG2850.016.228074
CGGTGAT1606.2937033E-1016.187514
CAATACT1151.2423716E-616.0869564
Produced by FastQC (version 0.11.5)