##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630602.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1270864
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.69577940676579	31.0	31.0	34.0	30.0	34.0
2	31.87907439348349	33.0	31.0	34.0	30.0	34.0
3	31.976551385514107	33.0	31.0	34.0	30.0	34.0
4	35.65471364363142	37.0	35.0	37.0	33.0	37.0
5	35.56352292613529	37.0	35.0	37.0	33.0	37.0
6	35.6385931146055	37.0	35.0	37.0	33.0	37.0
7	35.58830134459706	37.0	35.0	37.0	33.0	37.0
8	35.56960461544272	37.0	35.0	37.0	33.0	37.0
9	37.238617979579246	39.0	37.0	39.0	34.0	39.0
10	37.067806626043385	39.0	37.0	39.0	33.0	39.0
11	37.196272771909506	39.0	37.0	39.0	34.0	39.0
12	37.11909850306563	39.0	37.0	39.0	33.0	39.0
13	37.173230180412695	39.0	37.0	39.0	34.0	39.0
14	38.37412657845371	40.0	38.0	41.0	34.0	41.0
15	38.38494677636631	40.0	38.0	41.0	34.0	41.0
16	38.34957949867177	40.0	38.0	41.0	34.0	41.0
17	38.30212123405809	40.0	38.0	41.0	34.0	41.0
18	38.274270102859155	40.0	38.0	41.0	33.0	41.0
19	38.3370329161893	40.0	38.0	41.0	34.0	41.0
20	38.2978430422138	40.0	38.0	41.0	34.0	41.0
21	38.25380764582206	40.0	38.0	41.0	34.0	41.0
22	38.18672021553841	40.0	38.0	41.0	34.0	41.0
23	38.12832608367221	40.0	38.0	41.0	33.0	41.0
24	38.066150272570475	40.0	37.0	41.0	33.0	41.0
25	38.01407703735411	40.0	37.0	41.0	33.0	41.0
26	37.86399724911556	40.0	37.0	41.0	33.0	41.0
27	37.73322794571252	40.0	37.0	41.0	33.0	41.0
28	37.636997349834445	40.0	37.0	41.0	32.0	41.0
29	37.563180639313096	40.0	37.0	41.0	32.0	41.0
30	37.47445438693676	39.0	37.0	41.0	32.0	41.0
31	37.455157278827635	39.0	37.0	41.0	32.0	41.0
32	37.358424662277	39.0	36.0	41.0	32.0	41.0
33	37.29332642989336	39.0	36.0	41.0	31.0	41.0
34	37.24798798297851	39.0	36.0	41.0	31.0	41.0
35	37.20230410177643	39.0	36.0	40.0	31.0	41.0
36	37.187603866346045	39.0	36.0	40.0	31.0	41.0
37	37.07159696080777	39.0	36.0	40.0	31.0	41.0
38	36.92366610431958	39.0	36.0	40.0	31.0	41.0
39	36.8862726460109	39.0	36.0	40.0	31.0	41.0
40	36.72094338969394	39.0	35.0	40.0	30.0	41.0
41	36.691115650455124	39.0	35.0	40.0	30.0	41.0
42	36.57676352465724	39.0	35.0	40.0	30.0	41.0
43	35.71324154276146	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	1.0
10	3.0
11	5.0
12	1.0
13	4.0
14	2.0
15	2.0
16	6.0
17	18.0
18	50.0
19	115.0
20	247.0
21	414.0
22	764.0
23	1360.0
24	2208.0
25	3504.0
26	5457.0
27	7910.0
28	11219.0
29	15645.0
30	21275.0
31	27883.0
32	35926.0
33	46978.0
34	62059.0
35	84056.0
36	120377.0
37	190186.0
38	353832.0
39	279354.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.59058089614624	18.734970854473808	12.140952926512986	25.533495322866962
2	18.604901862040315	20.511872238099436	35.18755744123683	25.695668458623427
3	20.032670687028666	22.58738936660414	29.924523788540707	27.455416157826484
4	14.266986868775888	15.39330723035667	34.860142391318035	35.47956350954941
5	13.981275730526635	37.45444044366667	34.82142857142857	13.742855254378124
6	35.381126540684136	35.38569036498004	15.392913797227713	13.84026929710811
7	30.19016983721311	30.4549503330018	20.661298140477662	18.693581689307432
8	27.866711150839112	34.24111470621561	19.395151644865223	18.497022498080046
9	26.865030404512208	13.564866106837552	18.712938599252162	40.85716488939808
10	16.297495247327802	27.239106623525412	33.14375102292614	23.31964710622065
11	36.56292097344799	21.22736972642234	21.06141963262788	21.148289667501793
12	21.845453172015258	25.32906746906042	29.121920205466516	23.703559153457803
13	30.615234989739264	19.11125029900918	25.328201916176713	24.945312795074848
14	23.11372420652407	20.029601908622794	24.980485716803685	31.876188168049453
15	25.790328469450703	27.38184416271135	22.029579876367574	24.79824749147037
16	25.75051303680016	26.3815010890229	23.156765790832065	24.711220083344873
17	23.971408427652367	26.582309358043034	24.89747132659356	24.548810887711035
18	23.87533205756084	25.160442029989046	26.505117778141486	24.45910813430863
19	24.810837351597023	25.208440871721915	26.018440997620516	23.962280779060542
20	25.456461116217	24.28426645179972	25.953445844716665	24.305826587266615
21	25.45724798247491	24.962151732994247	24.98748882649914	24.593111458031704
22	25.330247768447293	24.792975487542336	25.17948419343061	24.697292550579764
23	24.599721134598195	24.969548275818656	25.66474461468733	24.76598597489582
24	24.76157952385149	25.376987624167498	25.42593070540986	24.43550214657115
25	25.06649019879389	24.883229047325283	25.435766533633814	24.614514220247013
26	24.67227020357804	25.56316018079039	25.40319027055609	24.361379345075477
27	25.34197207569024	24.677148774377116	25.059880522227395	24.920998627705245
28	24.378533029498044	24.940276851024183	25.922207254277406	24.75898286520037
29	23.979198403605736	25.36180110538972	26.019385237130017	24.63961525387453
30	24.035144594543556	26.059987536038477	25.805593674854272	24.099274194563698
31	24.76102871747095	25.585507182515205	25.12282982286067	24.530634277153183
32	23.969677321884955	25.27603268327689	25.650581022044843	25.103708972793314
33	24.02302685417165	25.008812902088657	26.109874856790345	24.85828538694935
34	24.806903020307445	24.69272872628385	25.928108751211777	24.572259502196932
35	24.693515592541768	24.99244608392401	26.22263279154969	24.091405531984538
36	24.10785103677498	25.639564894434024	25.700153596293546	24.55243047249745
37	25.075145727630964	24.766930214405317	25.654672726586007	24.503251331377708
38	24.312121517329942	24.788175603369048	26.410851200443165	24.48885167885785
39	24.612389681350642	24.78345440582155	25.850838484684434	24.753317428143372
40	23.719689911745082	24.692413979780685	26.825923151493786	24.76197295698045
41	23.28659872338818	24.72302307721361	27.29402988832794	24.696348311070267
42	24.190471993856146	24.34210112175654	27.024606881617547	24.442820002769768
43	23.499288672902843	23.903659242845812	27.195671606088457	25.40138047816289
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	28.0
1	34.5
2	41.0
3	129.0
4	217.0
5	217.0
6	346.0
7	475.0
8	495.5
9	516.0
10	773.5
11	1031.0
12	1031.0
13	1772.0
14	2513.0
15	4274.0
16	6035.0
17	5906.5
18	5778.0
19	5778.0
20	7086.5
21	8395.0
22	8905.0
23	9415.0
24	11414.5
25	13414.0
26	13414.0
27	16096.5
28	18779.0
29	23631.5
30	28484.0
31	32848.0
32	37212.0
33	37212.0
34	41557.0
35	45902.0
36	50852.0
37	55802.0
38	61924.5
39	68047.0
40	68047.0
41	71576.5
42	75106.0
43	74862.5
44	74619.0
45	78500.5
46	82382.0
47	82382.0
48	85179.5
49	87977.0
50	90358.5
51	92740.0
52	98547.0
53	104354.0
54	104354.0
55	97705.5
56	91057.0
57	86977.0
58	82897.0
59	76764.0
60	70631.0
61	70631.0
62	66228.5
63	61826.0
64	54578.0
65	47330.0
66	41251.0
67	35172.0
68	35172.0
69	29453.0
70	23734.0
71	20473.0
72	17212.0
73	13456.0
74	9700.0
75	9700.0
76	7509.0
77	5318.0
78	4234.5
79	3151.0
80	2379.5
81	1608.0
82	1608.0
83	1207.0
84	806.0
85	663.0
86	520.0
87	459.5
88	399.0
89	399.0
90	304.0
91	209.0
92	116.0
93	23.0
94	15.5
95	8.0
96	8.0
97	4.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1270864.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.182041743900164
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.82340269902055	55.488618954208704
2	7.764859382098519	9.811993392305268
3	1.7730732471592545	3.360791637510259
4	0.7440972170842853	1.88054325725357
5	0.40647372784840835	1.2840920020358426
6	0.2574235828773966	0.975872853553441
7	0.17422470614226596	0.7705510859409572
8	0.12974190443943368	0.6557886737780323
9	0.09602716809321316	0.5460473288712521
>10	0.6463589037720545	8.134535017892777
>50	0.09701971569175048	4.357760422687969
>100	0.08167772984545542	9.85761885627754
>500	0.004745791227662079	1.9565921935107644
>1k	8.742246998324883E-4	0.9191943241735319
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2797	0.2200864923390701	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2305	0.18137267244960908	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1821	0.14328834556648076	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	7.868662579158745E-5	0.0
2	0.0	0.0	0.0	7.081796321242871E-4	0.0
3	0.0	0.0	0.0	0.0011802993868738117	0.0
4	0.0	0.0	0.0	0.002439285399539211	0.0
5	0.0	1.573732515831749E-4	0.0	0.002596658651122386	0.0
6	0.0	1.573732515831749E-4	0.0	0.0026753452769139735	0.0
7	0.0	1.573732515831749E-4	0.0	0.002754031902705561	0.0
8	0.0	1.573732515831749E-4	0.0	0.002911405154288736	0.0
9	0.0	1.573732515831749E-4	0.0	0.004878570799078422	0.0
10	0.0	1.573732515831749E-4	0.0	0.008734215462866207	0.0
11	0.0	2.3605987737476236E-4	0.0	0.009993201475531607	0.0
12	0.0	2.3605987737476236E-4	0.0	0.010622694481864306	0.0
13	0.0	2.3605987737476236E-4	0.0	0.011252187488197006	0.0
14	0.0	2.3605987737476236E-4	0.0	0.011566933991363356	0.0
15	0.0	2.3605987737476236E-4	0.0	0.012432486875070817	0.0
16	0.0	2.3605987737476236E-4	0.0	0.01329803975877828	7.868662579158745E-5
17	0.0	2.3605987737476236E-4	0.0	0.01487177227461003	7.868662579158745E-5
18	0.0	2.3605987737476236E-4	0.0	0.015894698409900668	3.147465031663498E-4
19	0.0	3.147465031663498E-4	0.0	0.016524191416233364	3.147465031663498E-4
20	0.0	3.147465031663498E-4	0.0	0.01794055068048194	3.147465031663498E-4
21	0.0	3.147465031663498E-4	0.0	0.019829029699480038	3.147465031663498E-4
22	0.0	3.147465031663498E-4	0.0	0.02187488197006131	3.147465031663498E-4
23	0.0	3.9343312895793726E-4	0.0	0.02565184000805751	3.147465031663498E-4
24	0.0	3.9343312895793726E-4	0.0	0.031395963690843395	3.147465031663498E-4
25	0.0	4.721197547495247E-4	0.0	0.033520502587216255	3.147465031663498E-4
26	0.0	4.721197547495247E-4	0.0	0.037140087373629276	3.147465031663498E-4
27	0.0	4.721197547495247E-4	0.0	0.04146785179216659	3.147465031663498E-4
28	0.0	4.721197547495247E-4	0.0	0.0557888176862355	3.147465031663498E-4
29	0.0	5.508063805411122E-4	0.0	0.0796308653010865	3.147465031663498E-4
30	0.0	5.508063805411122E-4	0.0	0.11763650555842324	3.147465031663498E-4
31	0.0	5.508063805411122E-4	0.0	0.260924851124904	3.147465031663498E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGACGG	190	0.0	22.394737	5
GGTATCA	1100	0.0	20.854546	1
AGTCGGT	125	1.8189894E-11	20.720001	11
CGGACTA	55	5.1435054E-4	20.181818	24
TAATACT	255	0.0	18.862745	4
CCAATAC	120	5.175025E-9	18.5	3
TAGCCTA	70	1.2193261E-4	18.5	5
GACGGAC	200	0.0	18.5	7
CTAGGTA	50	0.00703528	18.5	4
TTAATAC	220	0.0	17.65909	3
GCAGTCG	160	3.45608E-11	17.34375	9
CAGTCGG	150	2.5102054E-10	17.266666	10
TCGCCAT	195	0.0	17.076923	13
GTATAGA	110	7.8091944E-7	16.818182	1
CGCAAAT	110	7.8091944E-7	16.818182	23
GAGCGAA	200	0.0	16.650002	16
CGAACTA	125	1.6580088E-7	16.28	29
CAAGACG	285	0.0	16.22807	4
CGGTGAT	160	6.2937033E-10	16.1875	14
CAATACT	115	1.2423716E-6	16.086956	4
>>END_MODULE