FastQCFastQC Report
Fri 10 Feb 2017
ERR1630601.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630601.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19770
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGT8204.147698533131006No Hit
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT6983.530601922104198TruSeq Adapter, Index 12 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATG4312.1800708143652No Hit
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCC3821.932220536165908No Hit
CATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC2891.4618108244815378No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2331.1785533636823469No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2221.1229135053110775No Hit
CTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTC2071.0470409711684372RNA PCR Primer, Index 12 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1300.6575619625695498No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA1100.556398583712696No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT830.4198280222559433No Hit
CACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCG640.32372281234193223No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC510.25796661608497723No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTC470.23773394031360648No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT450.2276176024279211No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA420.21244309559939303No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA390.19726858877086492No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA390.19726858877086492No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA350.1770359129994942No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG330.1669195751138088No Hit
TATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTAT330.1669195751138088No Hit
GGTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATA330.1669195751138088No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA300.15174506828528073No Hit
TTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTA290.14668689934243803No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC280.14162873039959534No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA260.13151239251390998No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT220.1112797167425392No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC210.10622154779969652No Hit
ACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT200.10116337885685382No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT200.10116337885685382No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTC200.001798488537.037
CTTATAC950.029.2105271
CCGTCTT900.028.77777737
GCCGTCT900.028.77777736
TTATACA1000.027.7499982
TATACAC1050.026.428573
TGGGCAG701.7321145E-723.7857156
ATACACA1250.022.1999994
CGGTGAT854.5636625E-821.76470614
TACACAT1400.021.1428595
CAGTCGG806.2768413E-720.812510
GGGCAGT806.2768413E-720.81257
GCAGTCG806.2768413E-720.81259
GTCGGTG806.2768413E-720.812512
TCGGTGA908.3548E-820.55555513
TGGGAAG450.003696236320.5555556
GGTATCA1900.020.4473691
GGTGATT758.534113E-619.73333215
GTGATTC758.534113E-619.73333216
AGTCGGT851.1237098E-619.58823611
Produced by FastQC (version 0.11.5)