##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630601.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	19770
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.79489124936773	31.0	31.0	34.0	30.0	34.0
2	31.955134041476985	33.0	31.0	34.0	30.0	34.0
3	31.933333333333334	33.0	31.0	34.0	30.0	34.0
4	35.677592311583204	37.0	35.0	37.0	33.0	37.0
5	35.65230146686899	37.0	35.0	37.0	33.0	37.0
6	35.73050075872534	37.0	35.0	37.0	35.0	37.0
7	35.622306525037935	37.0	35.0	37.0	33.0	37.0
8	35.70733434496712	37.0	35.0	37.0	33.0	37.0
9	37.357713707637835	39.0	37.0	39.0	34.0	39.0
10	37.252655538694995	39.0	37.0	39.0	34.0	39.0
11	37.35751138088012	39.0	37.0	39.0	34.0	39.0
12	37.30283257460799	39.0	37.0	39.0	34.0	39.0
13	37.39317147192716	39.0	37.0	39.0	34.0	39.0
14	38.46413758219524	40.0	38.0	41.0	34.0	41.0
15	38.420333839150224	40.0	38.0	41.0	34.0	41.0
16	38.29054122407688	40.0	38.0	41.0	33.0	41.0
17	38.28619119878604	40.0	38.0	41.0	34.0	41.0
18	38.41431461810824	40.0	38.0	41.0	34.0	41.0
19	38.43131006575619	40.0	38.0	41.0	34.0	41.0
20	38.453566009104705	40.0	38.0	41.0	34.0	41.0
21	38.4096105209914	40.0	38.0	41.0	34.0	41.0
22	38.20394537177542	40.0	38.0	41.0	33.0	41.0
23	38.18947900859889	40.0	38.0	41.0	33.0	41.0
24	38.074810318664646	40.0	37.0	41.0	33.0	41.0
25	37.9350531107739	40.0	37.0	41.0	33.0	41.0
26	37.8202832574608	40.0	37.0	41.0	33.0	41.0
27	37.522104198280225	40.0	37.0	41.0	32.0	41.0
28	37.52893272635306	40.0	36.0	41.0	32.0	41.0
29	37.342994436014166	39.0	36.0	41.0	31.0	41.0
30	37.16681841173495	39.0	36.0	41.0	31.0	41.0
31	37.05265553869499	39.0	35.0	41.0	31.0	41.0
32	36.75680323722813	39.0	35.0	40.0	31.0	41.0
33	36.56965098634294	39.0	35.0	40.0	30.0	41.0
34	36.52443095599393	39.0	35.0	40.0	30.0	41.0
35	36.37147192716237	39.0	35.0	40.0	30.0	41.0
36	36.39210925644917	39.0	35.0	40.0	30.0	41.0
37	36.19433485078402	39.0	35.0	40.0	30.0	41.0
38	36.0453717754173	38.0	35.0	40.0	29.0	41.0
39	35.898988366211434	38.0	35.0	40.0	28.0	41.0
40	35.478502781992916	38.0	34.0	40.0	27.0	41.0
41	35.40030349013657	38.0	34.0	40.0	27.0	41.0
42	35.15639858371269	38.0	34.0	40.0	26.0	41.0
43	34.063935255437535	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	2.0
15	0.0
16	0.0
17	2.0
18	0.0
19	1.0
20	3.0
21	8.0
22	18.0
23	37.0
24	47.0
25	64.0
26	120.0
27	167.0
28	248.0
29	331.0
30	436.0
31	498.0
32	659.0
33	758.0
34	998.0
35	1390.0
36	1895.0
37	2886.0
38	4468.0
39	4732.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.448659585230146	18.002023267577137	15.442589782498736	27.106727364693985
2	18.012139605462824	18.10318664643399	36.317653009610524	27.567020738492666
3	18.219524532119372	23.338391502276178	35.39706626201315	23.0450177035913
4	12.038442083965604	19.084471421345473	26.666666666666668	42.21041982802225
5	13.297926150733435	36.14567526555387	33.803743045017704	16.752655538694995
6	31.58320687910976	30.748609003540718	17.116843702579665	20.551340414769854
7	25.639858371269604	32.943854324734446	15.97875569044006	25.43753161355589
8	32.690945877592306	29.276681841173495	19.054122407688418	18.978249873545778
9	23.899848254931715	10.748609003540718	15.366717248356096	49.98482549317147
10	19.21092564491654	26.1608497723824	24.24886191198786	30.3793626707132
11	31.264542235710675	19.853313100657562	19.347496206373293	29.534648457258474
12	20.9357612544259	29.736975214972176	21.224076884167932	28.103186646433993
13	35.30096105209914	14.375316135558927	25.240263024785026	25.083459787556905
14	23.485078401618615	22.175012645422356	21.48204350025291	32.85786545270612
15	31.901871522508852	22.13960546282246	17.880627212948912	28.077895801719777
16	31.52250885179565	20.591805766312596	22.129489124936775	25.756196256954983
17	19.02377339403136	34.59281740010116	20.556398583712696	25.827010622154777
18	28.143651997976733	17.06626201315124	23.540718259989884	31.249367728882145
19	27.13707637835104	26.00910470409712	22.964087000505817	23.889731917046028
20	24.69903894790086	18.79615579160344	25.311077389984828	31.193727870510873
21	34.96712190187152	16.777946383409205	23.83409205867476	24.420839656044514
22	24.36519979767324	26.403641881638844	25.03793626707132	24.19322205361659
23	27.59736975214972	22.08396560445119	21.588265048052605	28.730399595346483
24	30.47546788062721	21.664137582195245	27.000505816894282	20.859888720283255
25	20.713201820940817	26.079919069296913	26.226605968639355	26.98027314112291
26	33.034901365705615	22.190187152250886	22.9742033383915	21.800708143652
27	21.289833080424884	26.71724835609509	26.82852807283763	25.164390490642386
28	23.25240263024785	21.583206879109763	27.42539200809307	27.738998482549317
29	26.383409205867476	20.687910976226608	28.376327769347498	24.552352048558422
30	20.18209408194234	22.362164896307537	32.883156297420335	24.572584724329793
31	27.683358624178044	22.81740010116338	23.287809812847748	26.211431461810825
32	22.76176024279211	22.564491654021243	34.14264036418817	20.531107738998482
33	20.480526049570056	26.31259484066768	31.138088012139605	22.06879109762266
34	26.77794638340921	25.97369752149722	23.72281234193222	23.525543753161354
35	20.92058674759737	21.67425392008093	36.393525543753164	21.01163378856854
36	23.464845725847244	26.423874557410215	23.399089529590288	26.712190187152252
37	21.102680829539707	25.776428932726354	27.4759736975215	25.644916540212442
38	21.93727870510875	20.13657056145675	30.69802731411229	27.228123419322202
39	25.91805766312595	21.583206879109763	25.48305513404148	27.015680323722812
40	19.49418310571573	19.438543247344462	35.80677794638341	25.2604957005564
41	21.37076378351037	22.604957005563985	25.230146686899342	30.794132524026303
42	21.340414769853314	19.93930197268589	33.788568538189175	24.931714719271625
43	19.802731411229136	21.45169448659585	32.640364188163886	26.10520991401113
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	9.5
4	18.0
5	18.0
6	32.5
7	47.0
8	49.5
9	52.0
10	60.0
11	68.0
12	68.0
13	114.5
14	161.0
15	276.0
16	391.0
17	332.5
18	274.0
19	274.0
20	327.0
21	380.0
22	254.5
23	129.0
24	116.5
25	104.0
26	104.0
27	131.5
28	159.0
29	169.0
30	179.0
31	190.5
32	202.0
33	202.0
34	227.0
35	252.0
36	292.5
37	333.0
38	378.0
39	423.0
40	423.0
41	489.5
42	556.0
43	627.5
44	699.0
45	881.0
46	1063.0
47	1063.0
48	1394.0
49	1725.0
50	2337.5
51	2950.0
52	2061.0
53	1172.0
54	1172.0
55	1655.0
56	2138.0
57	1836.0
58	1534.0
59	1206.5
60	879.0
61	879.0
62	852.0
63	825.0
64	780.5
65	736.0
66	690.0
67	644.0
68	644.0
69	578.5
70	513.0
71	478.5
72	444.0
73	391.5
74	339.0
75	339.0
76	257.5
77	176.0
78	148.0
79	120.0
80	88.0
81	56.0
82	56.0
83	36.0
84	16.0
85	12.0
86	8.0
87	5.5
88	3.0
89	3.0
90	2.0
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	19770.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.46737481031866
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.903073286052	62.92362164896308
2	5.319148936170213	7.283763277693475
3	1.2263593380614657	2.5189681335356604
4	0.583628841607565	1.5983813859382905
5	0.20685579196217493	0.7081436519979768
6	0.14036643026004728	0.5766312594840668
7	0.08126477541371158	0.38947900859888723
8	0.08126477541371158	0.4451188669701568
9	0.03693853427895981	0.2276176024279211
>10	0.3250591016548463	4.51188669701568
>50	0.022163120567375887	1.0015174506828528
>100	0.0591016548463357	10.136570561456752
>500	0.014775413711583925	7.678300455235205
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGT	820	4.147698533131006	No Hit
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT	698	3.530601922104198	TruSeq Adapter, Index 12 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATG	431	2.1800708143652	No Hit
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCC	382	1.932220536165908	No Hit
CATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC	289	1.4618108244815378	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	233	1.1785533636823469	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	222	1.1229135053110775	No Hit
CTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTC	207	1.0470409711684372	RNA PCR Primer, Index 12 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	130	0.6575619625695498	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	110	0.556398583712696	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	83	0.4198280222559433	No Hit
CACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCG	64	0.32372281234193223	No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC	51	0.25796661608497723	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTC	47	0.23773394031360648	No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT	45	0.2276176024279211	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA	42	0.21244309559939303	No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA	39	0.19726858877086492	No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA	39	0.19726858877086492	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA	35	0.1770359129994942	No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG	33	0.1669195751138088	No Hit
TATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTAT	33	0.1669195751138088	No Hit
GGTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATA	33	0.1669195751138088	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	30	0.15174506828528073	No Hit
TTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTA	29	0.14668689934243803	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC	28	0.14162873039959534	No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA	26	0.13151239251390998	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22	0.1112797167425392	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC	21	0.10622154779969652	No Hit
ACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT	20	0.10116337885685382	No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT	20	0.10116337885685382	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.005058168942842691	0.0
2	0.0	0.0	0.0	0.015174506828528073	0.0
3	0.0	0.0	0.0	0.035407182599898834	0.0
4	0.0	0.0	0.0	0.04046535154274153	0.0
5	0.0	0.0	0.0	0.04552352048558422	0.0
6	0.0	0.0	0.0	0.05058168942842691	0.0
7	0.0	0.0	0.0	0.0556398583712696	0.0
8	0.0	0.0	0.0	0.0556398583712696	0.0
9	0.0	0.0	0.0	0.09104704097116843	0.0
10	0.0	0.0	0.0	0.14162873039959534	0.0
11	0.0	0.0	0.0	0.15680323722812342	0.0
12	0.0	0.0	0.0	0.15680323722812342	0.0
13	0.0	0.0	0.0	0.16186140617096612	0.0
14	0.0	0.0	0.0	0.17703591299949417	0.0
15	0.0	0.0	0.0	0.18715225088517956	0.0
16	0.0	0.0	0.0	0.19726858877086495	0.0
17	0.0	0.0	0.0	0.19726858877086495	0.0
18	0.0	0.0	0.0	0.20232675771370764	0.0
19	0.0	0.0	0.0	0.2175012645422357	0.0
20	0.0	0.0	0.0	0.2276176024279211	0.0
21	0.0	0.0	0.0	0.24279210925644917	0.0
22	0.0	0.0	0.0	0.278199291856348	0.0
23	0.0	0.0	0.0	0.278199291856348	0.0
24	0.0	0.0	0.0	0.343955488113303	0.0
25	0.0	0.0	0.0	0.36418816388467373	0.0
26	0.0	0.0	0.0	0.37936267071320184	0.0
27	0.0	0.0	0.0	0.3844208396560445	0.0
28	0.0	0.0	0.0	0.4400606980273141	0.0
29	0.0	0.0	0.0	0.5209914011127972	0.0
30	0.0	0.0	0.0	0.6170966110268084	0.0
31	0.0	0.0	0.0	1.031866464339909	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTTC	20	0.0017984885	37.0	37
CTTATAC	95	0.0	29.210527	1
CCGTCTT	90	0.0	28.777777	37
GCCGTCT	90	0.0	28.777777	36
TTATACA	100	0.0	27.749998	2
TATACAC	105	0.0	26.42857	3
TGGGCAG	70	1.7321145E-7	23.785715	6
ATACACA	125	0.0	22.199999	4
CGGTGAT	85	4.5636625E-8	21.764706	14
TACACAT	140	0.0	21.142859	5
CAGTCGG	80	6.2768413E-7	20.8125	10
GGGCAGT	80	6.2768413E-7	20.8125	7
GCAGTCG	80	6.2768413E-7	20.8125	9
GTCGGTG	80	6.2768413E-7	20.8125	12
TCGGTGA	90	8.3548E-8	20.555555	13
TGGGAAG	45	0.0036962363	20.555555	6
GGTATCA	190	0.0	20.447369	1
GGTGATT	75	8.534113E-6	19.733332	15
GTGATTC	75	8.534113E-6	19.733332	16
AGTCGGT	85	1.1237098E-6	19.588236	11
>>END_MODULE