FastQCFastQC Report
Fri 10 Feb 2017
ERR1630600.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630600.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1630421
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT128500.7881399957434306No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT110220.6760217146368943No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT101240.6209439157125675No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70710.43369166614021776No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA21200.13002776583471387No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20740.12720640865150779No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT19260.11812899858380135No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18170.11144360873663918No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC17700.1085609177016243No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCGTA555.144363E-420.18181812
TCCGTAT555.144363E-420.18181813
CCGTATT609.239079E-418.514
CCGCTTA953.608262E-617.52631425
CGTAAGA650.001580317617.0769232
AAGTACG2500.017.0235
AATGCGT1801.0913936E-1116.44444535
ACGGACC1951.8189894E-1216.1282068
ATTCCGT1504.6748028E-916.03333329
GGTATCA63650.015.9277291
CATCCGT700.002593117315.85714311
TCTATAC1751.3278623E-1015.8571423
GCCGTCT1557.214112E-915.51612936
GACGGAC2055.456968E-1215.3414647
CGCAAGA2201.8189894E-1215.1363642
CTAATAC4800.015.0312493
AAATGCG1853.0559022E-1015.034
GCGAACT3250.014.828
CGTTTAG2251.8189894E-1214.826
TCCGAAT1752.239176E-914.79999912
Produced by FastQC (version 0.11.5)